Effective control of biofilms by photothermal therapy using a gold nanorod hydrogel
Biofilms are matrices synthesized by bacteria containing polysaccharides, DNA, and proteins. The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2020-02, Vol.108 (2), p.333-342 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Bermúdez‐Jiménez, Carlos Niño‐Martínez, Nereyda Patiño‐Marín, Nuria Martínez‐Gutiérrez, Fidel Ruiz, Facundo Bach, Horacio Martínez‐Castañón, Gabriel |
| description | Biofilms are matrices synthesized by bacteria containing polysaccharides, DNA, and proteins. The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nanorods (AuNRs) is an attractive treatment because of its anti‐biofilm activity. The purpose of this work was to generate a novel chitosan‐based hydrogel embedded with AuNRs to evaluate its anti‐biofilm activity. AuNRs were synthesized by the seed‐mediated growth method and mixed with the chitosan‐based hydrogel. Hydrogels were characterized and tested against two bacterial strains by irradiating the produced biofilm in the presence of the nanoformulation with a laser adjusted at the near infrared spectrum. In addition, the safety of the nanoformulation was assessed with normal human gingival fibroblasts. Results showed that a significant bacterial killing was measured when biofilms were exposed to an increase of 10°C for a short time of 2 min. Moreover, no cytotoxicity was measured when normal gingival fibroblasts were exposed to the nanoformulation using the bactericidal conditions. The development of the reported formulation can be used as a direct application to treat periodontal diseases or biofilm‐produced bacteria that colonize the oral cavity. |
| doi_str_mv | 10.1002/jbm.b.34392 |
| format | Article |
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The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nanorods (AuNRs) is an attractive treatment because of its anti‐biofilm activity. The purpose of this work was to generate a novel chitosan‐based hydrogel embedded with AuNRs to evaluate its anti‐biofilm activity. AuNRs were synthesized by the seed‐mediated growth method and mixed with the chitosan‐based hydrogel. Hydrogels were characterized and tested against two bacterial strains by irradiating the produced biofilm in the presence of the nanoformulation with a laser adjusted at the near infrared spectrum. In addition, the safety of the nanoformulation was assessed with normal human gingival fibroblasts. Results showed that a significant bacterial killing was measured when biofilms were exposed to an increase of 10°C for a short time of 2 min. Moreover, no cytotoxicity was measured when normal gingival fibroblasts were exposed to the nanoformulation using the bactericidal conditions. The development of the reported formulation can be used as a direct application to treat periodontal diseases or biofilm‐produced bacteria that colonize the oral cavity.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.34392</identifier><identifier>PMID: 31041849</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Antibiotics ; Bacteria ; biofilm ; Biofilms ; Biomedical materials ; Chitosan ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; E faecalis ; Fibroblasts ; gingival fibroblasts ; Gold ; gold nanorods ; Hydrogels ; Inflammation ; Inflammatory response ; Materials research ; Materials science ; Nanorods ; Near infrared radiation ; Oral cavity ; Periodontal diseases ; photothermal therapy ; Polysaccharides ; S oralis ; Saccharides ; Synthesis ; Therapy ; Toxicity</subject><ispartof>Journal of biomedical materials research. 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Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>Biofilms are matrices synthesized by bacteria containing polysaccharides, DNA, and proteins. The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nanorods (AuNRs) is an attractive treatment because of its anti‐biofilm activity. The purpose of this work was to generate a novel chitosan‐based hydrogel embedded with AuNRs to evaluate its anti‐biofilm activity. AuNRs were synthesized by the seed‐mediated growth method and mixed with the chitosan‐based hydrogel. Hydrogels were characterized and tested against two bacterial strains by irradiating the produced biofilm in the presence of the nanoformulation with a laser adjusted at the near infrared spectrum. In addition, the safety of the nanoformulation was assessed with normal human gingival fibroblasts. Results showed that a significant bacterial killing was measured when biofilms were exposed to an increase of 10°C for a short time of 2 min. Moreover, no cytotoxicity was measured when normal gingival fibroblasts were exposed to the nanoformulation using the bactericidal conditions. The development of the reported formulation can be used as a direct application to treat periodontal diseases or biofilm‐produced bacteria that colonize the oral cavity.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biomedical materials</subject><subject>Chitosan</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E faecalis</subject><subject>Fibroblasts</subject><subject>gingival fibroblasts</subject><subject>Gold</subject><subject>gold nanorods</subject><subject>Hydrogels</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Nanorods</subject><subject>Near infrared radiation</subject><subject>Oral cavity</subject><subject>Periodontal diseases</subject><subject>photothermal therapy</subject><subject>Polysaccharides</subject><subject>S oralis</subject><subject>Saccharides</subject><subject>Synthesis</subject><subject>Therapy</subject><subject>Toxicity</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp90DtPwzAUBWALgWgpTOzIEgsSavEjL4-0Ki8VMQCzZTt2m8qJi52A8u9JSenAwHTu8Ono6gBwjtEEI0Ru1rKcyAmNKCMHYIjjmIwjluHD_Z3SATgJYd3hBMX0GAwoRhHOIjYEr3NjtKqLTw2Vq2rvLHQGysKZwpYByhZuVq529Ur7Uli4TbFpYROKagkFXDqbw0pUzrscrtrcu6W2p-DICBv02S5H4P1u_jZ7GC9e7h9nt4uxoiwlY8kUFgwxwVhupMQpQxQRFQmWpSbOKKYZM2mikEQ4yZSOcS7ShCW5yJQRKqcjcNX3brz7aHSoeVkEpa0VlXZN4ITgjBKcRKijl3_o2jW-6r7jhFIaR0mMk05d90p5F4LXhm98UQrfcoz4dmvebc0l_9m60xe7zkaWOt_b33E7QHrwVVjd_tfFn6bP0771G-NCiZs</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Bermúdez‐Jiménez, Carlos</creator><creator>Niño‐Martínez, Nereyda</creator><creator>Patiño‐Marín, Nuria</creator><creator>Martínez‐Gutiérrez, Fidel</creator><creator>Ruiz, Facundo</creator><creator>Bach, Horacio</creator><creator>Martínez‐Castañón, Gabriel</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7503-350X</orcidid></search><sort><creationdate>202002</creationdate><title>Effective control of biofilms by photothermal therapy using a gold nanorod hydrogel</title><author>Bermúdez‐Jiménez, Carlos ; Niño‐Martínez, Nereyda ; Patiño‐Marín, Nuria ; Martínez‐Gutiérrez, Fidel ; Ruiz, Facundo ; Bach, Horacio ; Martínez‐Castañón, Gabriel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3972-b9c1a909a99dfbb1790302c4a987f5831389f76c0b0168ce51da7696da8cfacd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biomedical materials</topic><topic>Chitosan</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E faecalis</topic><topic>Fibroblasts</topic><topic>gingival fibroblasts</topic><topic>Gold</topic><topic>gold nanorods</topic><topic>Hydrogels</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Nanorods</topic><topic>Near infrared radiation</topic><topic>Oral cavity</topic><topic>Periodontal diseases</topic><topic>photothermal therapy</topic><topic>Polysaccharides</topic><topic>S oralis</topic><topic>Saccharides</topic><topic>Synthesis</topic><topic>Therapy</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bermúdez‐Jiménez, Carlos</creatorcontrib><creatorcontrib>Niño‐Martínez, Nereyda</creatorcontrib><creatorcontrib>Patiño‐Marín, Nuria</creatorcontrib><creatorcontrib>Martínez‐Gutiérrez, Fidel</creatorcontrib><creatorcontrib>Ruiz, Facundo</creatorcontrib><creatorcontrib>Bach, Horacio</creatorcontrib><creatorcontrib>Martínez‐Castañón, Gabriel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. 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The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nanorods (AuNRs) is an attractive treatment because of its anti‐biofilm activity. The purpose of this work was to generate a novel chitosan‐based hydrogel embedded with AuNRs to evaluate its anti‐biofilm activity. AuNRs were synthesized by the seed‐mediated growth method and mixed with the chitosan‐based hydrogel. Hydrogels were characterized and tested against two bacterial strains by irradiating the produced biofilm in the presence of the nanoformulation with a laser adjusted at the near infrared spectrum. In addition, the safety of the nanoformulation was assessed with normal human gingival fibroblasts. Results showed that a significant bacterial killing was measured when biofilms were exposed to an increase of 10°C for a short time of 2 min. Moreover, no cytotoxicity was measured when normal gingival fibroblasts were exposed to the nanoformulation using the bactericidal conditions. The development of the reported formulation can be used as a direct application to treat periodontal diseases or biofilm‐produced bacteria that colonize the oral cavity.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>31041849</pmid><doi>10.1002/jbm.b.34392</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7503-350X</orcidid></addata></record> |
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| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2020-02, Vol.108 (2), p.333-342 |
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| source | Wiley Online Library All Journals |
| subjects | Antibiotics Bacteria biofilm Biofilms Biomedical materials Chitosan Cytotoxicity Deoxyribonucleic acid DNA E faecalis Fibroblasts gingival fibroblasts Gold gold nanorods Hydrogels Inflammation Inflammatory response Materials research Materials science Nanorods Near infrared radiation Oral cavity Periodontal diseases photothermal therapy Polysaccharides S oralis Saccharides Synthesis Therapy Toxicity |
| title | Effective control of biofilms by photothermal therapy using a gold nanorod hydrogel |
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