CD8 + XCR1 neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors
Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8 and CD8 subsets. It is well-established that CD8 dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8 DCs a...
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| Veröffentlicht in: | Frontiers in immunology 2018, Vol.9, p.2990 |
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| container_title | Frontiers in immunology |
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| creator | Wylie, Ben Read, James Buzzai, Anthony C Wagner, Teagan Troy, Niamh Syn, Genevieve Stone, Shane R Foley, Bree Bosco, Anthony Cruickshank, Mark N Waithman, Jason |
| description | Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8
and CD8
subsets. It is well-established that CD8
dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8
DCs are grouped together in the heterogeneous cDC2 subset. CD8
DCs are relatively poor cross-presenters and drive more prominent CD4
T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8
DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8
XCR1
DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8
XCR1
DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network. |
| doi_str_mv | 10.3389/fimmu.2018.02990 |
| format | Article |
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and CD8
subsets. It is well-established that CD8
dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8
DCs are grouped together in the heterogeneous cDC2 subset. CD8
DCs are relatively poor cross-presenters and drive more prominent CD4
T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8
DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8
XCR1
DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8
XCR1
DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network.</description><identifier>EISSN: 1664-3224</identifier><identifier>DOI: 10.3389/fimmu.2018.02990</identifier><identifier>PMID: 30700986</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Animals ; CD8 Antigens - metabolism ; Cell Separation ; Cross-Priming ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; Endocytosis - immunology ; Flow Cytometry ; Gene Expression Profiling ; Mice ; Mice, Inbred C57BL ; Oligonucleotide Array Sequence Analysis ; Receptors, Chemokine - metabolism ; Receptors, Pattern Recognition - immunology ; Receptors, Pattern Recognition - metabolism ; Toll-Like Receptor 5 - immunology ; Toll-Like Receptor 5 - metabolism</subject><ispartof>Frontiers in immunology, 2018, Vol.9, p.2990</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30700986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wylie, Ben</creatorcontrib><creatorcontrib>Read, James</creatorcontrib><creatorcontrib>Buzzai, Anthony C</creatorcontrib><creatorcontrib>Wagner, Teagan</creatorcontrib><creatorcontrib>Troy, Niamh</creatorcontrib><creatorcontrib>Syn, Genevieve</creatorcontrib><creatorcontrib>Stone, Shane R</creatorcontrib><creatorcontrib>Foley, Bree</creatorcontrib><creatorcontrib>Bosco, Anthony</creatorcontrib><creatorcontrib>Cruickshank, Mark N</creatorcontrib><creatorcontrib>Waithman, Jason</creatorcontrib><title>CD8 + XCR1 neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors</title><title>Frontiers in immunology</title><addtitle>Front Immunol</addtitle><description>Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8
and CD8
subsets. It is well-established that CD8
dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8
DCs are grouped together in the heterogeneous cDC2 subset. CD8
DCs are relatively poor cross-presenters and drive more prominent CD4
T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8
DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8
XCR1
DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8
XCR1
DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network.</description><subject>Animals</subject><subject>CD8 Antigens - metabolism</subject><subject>Cell Separation</subject><subject>Cross-Priming</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - metabolism</subject><subject>Endocytosis - immunology</subject><subject>Flow Cytometry</subject><subject>Gene Expression Profiling</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Receptors, Chemokine - metabolism</subject><subject>Receptors, Pattern Recognition - immunology</subject><subject>Receptors, Pattern Recognition - metabolism</subject><subject>Toll-Like Receptor 5 - immunology</subject><subject>Toll-Like Receptor 5 - metabolism</subject><issn>1664-3224</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjk1rwkAURYdCUWnduypvX5JOMjHNrMeIC1eSgjuJyYuddr46k5Tm36ug697NgcvhcglZJDRmrOBvndR6iFOaFDFNOacPZJbkeRaxNM2mZB7CF70k44yx5YRMGX2nlBf5jIxiVcAr7MUuAYMnWKFpvexlAwKVClD-OY8hwEaePmGLv3jpbAeVVSraym-EHTboeuthCbVpoYYPI38GBGG1U6jR9Fe_NK1txuvs3Q_P5LGrVcD5jU_kZV1WYhO54aixPTgvde3Hw_0r-1c4AzdWTyQ</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Wylie, Ben</creator><creator>Read, James</creator><creator>Buzzai, Anthony C</creator><creator>Wagner, Teagan</creator><creator>Troy, Niamh</creator><creator>Syn, Genevieve</creator><creator>Stone, Shane R</creator><creator>Foley, Bree</creator><creator>Bosco, Anthony</creator><creator>Cruickshank, Mark N</creator><creator>Waithman, Jason</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>2018</creationdate><title>CD8 + XCR1 neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors</title><author>Wylie, Ben ; Read, James ; Buzzai, Anthony C ; Wagner, Teagan ; Troy, Niamh ; Syn, Genevieve ; Stone, Shane R ; Foley, Bree ; Bosco, Anthony ; Cruickshank, Mark N ; Waithman, Jason</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_307009863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>CD8 Antigens - metabolism</topic><topic>Cell Separation</topic><topic>Cross-Priming</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>Endocytosis - immunology</topic><topic>Flow Cytometry</topic><topic>Gene Expression Profiling</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Receptors, Chemokine - metabolism</topic><topic>Receptors, Pattern Recognition - immunology</topic><topic>Receptors, Pattern Recognition - metabolism</topic><topic>Toll-Like Receptor 5 - immunology</topic><topic>Toll-Like Receptor 5 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wylie, Ben</creatorcontrib><creatorcontrib>Read, James</creatorcontrib><creatorcontrib>Buzzai, Anthony C</creatorcontrib><creatorcontrib>Wagner, Teagan</creatorcontrib><creatorcontrib>Troy, Niamh</creatorcontrib><creatorcontrib>Syn, Genevieve</creatorcontrib><creatorcontrib>Stone, Shane R</creatorcontrib><creatorcontrib>Foley, Bree</creatorcontrib><creatorcontrib>Bosco, Anthony</creatorcontrib><creatorcontrib>Cruickshank, Mark N</creatorcontrib><creatorcontrib>Waithman, Jason</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Frontiers in immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wylie, Ben</au><au>Read, James</au><au>Buzzai, Anthony C</au><au>Wagner, Teagan</au><au>Troy, Niamh</au><au>Syn, Genevieve</au><au>Stone, Shane R</au><au>Foley, Bree</au><au>Bosco, Anthony</au><au>Cruickshank, Mark N</au><au>Waithman, Jason</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD8 + XCR1 neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors</atitle><jtitle>Frontiers in immunology</jtitle><addtitle>Front Immunol</addtitle><date>2018</date><risdate>2018</risdate><volume>9</volume><spage>2990</spage><pages>2990-</pages><eissn>1664-3224</eissn><abstract>Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8
and CD8
subsets. It is well-established that CD8
dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8
DCs are grouped together in the heterogeneous cDC2 subset. CD8
DCs are relatively poor cross-presenters and drive more prominent CD4
T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8
DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8
XCR1
DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8
XCR1
DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network.</abstract><cop>Switzerland</cop><pmid>30700986</pmid><doi>10.3389/fimmu.2018.02990</doi></addata></record> |
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| ispartof | Frontiers in immunology, 2018, Vol.9, p.2990 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Animals CD8 Antigens - metabolism Cell Separation Cross-Priming Dendritic Cells - immunology Dendritic Cells - metabolism Endocytosis - immunology Flow Cytometry Gene Expression Profiling Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Receptors, Chemokine - metabolism Receptors, Pattern Recognition - immunology Receptors, Pattern Recognition - metabolism Toll-Like Receptor 5 - immunology Toll-Like Receptor 5 - metabolism |
| title | CD8 + XCR1 neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors |
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