Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO 2 , and CH 4 fluxes in temperate rainforest soil
The Pacific coastal temperate rainforest (PCTR) is a global hot-spot for carbon cycling and export. Yet the influence of microorganisms on carbon cycling processes in PCTR soil is poorly characterized. We developed and tested a conceptual model of seasonal microbial carbon cycling in PCTR soil throu...
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| Veröffentlicht in: | The ISME Journal 2019-04, Vol.13 (4), p.950 |
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| creator | Levy-Booth, David J Giesbrecht, Ian J W Kellogg, Colleen T E Heger, Thierry J D'Amore, David V Keeling, Patrick J Hallam, Steven J Mohn, William W |
| description | The Pacific coastal temperate rainforest (PCTR) is a global hot-spot for carbon cycling and export. Yet the influence of microorganisms on carbon cycling processes in PCTR soil is poorly characterized. We developed and tested a conceptual model of seasonal microbial carbon cycling in PCTR soil through integration of geochemistry, micro-meteorology, and eukaryotic and prokaryotic ribosomal amplicon (rRNA) sequencing from 216 soil DNA and RNA libraries. Soil moisture and pH increased during the wet season, with significant correlation to net CO
flux in peat bog and net CH
flux in bog forest soil. Fungal succession in these sites was characterized by the apparent turnover of Archaeorhizomycetes phylotypes accounting for 41% of ITS libraries. Anaerobic prokaryotes, including Syntrophobacteraceae and Methanomicrobia increased in rRNA libraries during the wet season. Putatively active populations of these phylotypes and their biogeochemical marker genes for sulfate and CH
cycling, respectively, were positively correlated following rRNA and metatranscriptomic network analysis. The latter phylotype was positively correlated to CH
fluxes (r = 0.46, p |
| doi_str_mv | 10.1038/s41396-018-0334-3 |
| format | Article |
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flux in peat bog and net CH
flux in bog forest soil. Fungal succession in these sites was characterized by the apparent turnover of Archaeorhizomycetes phylotypes accounting for 41% of ITS libraries. Anaerobic prokaryotes, including Syntrophobacteraceae and Methanomicrobia increased in rRNA libraries during the wet season. Putatively active populations of these phylotypes and their biogeochemical marker genes for sulfate and CH
cycling, respectively, were positively correlated following rRNA and metatranscriptomic network analysis. The latter phylotype was positively correlated to CH
fluxes (r = 0.46, p < 0.0001). Phylotype functional assignments were supported by metatranscriptomic analysis. We propose that active microbial populations respond primarily to changes in hydrology, pH, and nutrient availability. The increased microbial carbon export observed over winter may have ramifications for climate-soil feedbacks in the PCTR.</description><identifier>EISSN: 1751-7370</identifier><identifier>DOI: 10.1038/s41396-018-0334-3</identifier><identifier>PMID: 30538276</identifier><language>eng</language><publisher>England</publisher><subject>Carbon Cycle ; Carbon Dioxide - analysis ; Climate ; Gene Expression Profiling ; Methane - analysis ; Northwestern United States ; Rainforest ; Seasons ; Soil - chemistry ; Soil Microbiology</subject><ispartof>The ISME Journal, 2019-04, Vol.13 (4), p.950</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-7013-4766 ; 0000-0003-3614-0964 ; 0000-0002-7644-0745 ; 0000-0002-4889-6876</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30538276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levy-Booth, David J</creatorcontrib><creatorcontrib>Giesbrecht, Ian J W</creatorcontrib><creatorcontrib>Kellogg, Colleen T E</creatorcontrib><creatorcontrib>Heger, Thierry J</creatorcontrib><creatorcontrib>D'Amore, David V</creatorcontrib><creatorcontrib>Keeling, Patrick J</creatorcontrib><creatorcontrib>Hallam, Steven J</creatorcontrib><creatorcontrib>Mohn, William W</creatorcontrib><title>Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO 2 , and CH 4 fluxes in temperate rainforest soil</title><title>The ISME Journal</title><addtitle>ISME J</addtitle><description>The Pacific coastal temperate rainforest (PCTR) is a global hot-spot for carbon cycling and export. Yet the influence of microorganisms on carbon cycling processes in PCTR soil is poorly characterized. We developed and tested a conceptual model of seasonal microbial carbon cycling in PCTR soil through integration of geochemistry, micro-meteorology, and eukaryotic and prokaryotic ribosomal amplicon (rRNA) sequencing from 216 soil DNA and RNA libraries. Soil moisture and pH increased during the wet season, with significant correlation to net CO
flux in peat bog and net CH
flux in bog forest soil. Fungal succession in these sites was characterized by the apparent turnover of Archaeorhizomycetes phylotypes accounting for 41% of ITS libraries. Anaerobic prokaryotes, including Syntrophobacteraceae and Methanomicrobia increased in rRNA libraries during the wet season. Putatively active populations of these phylotypes and their biogeochemical marker genes for sulfate and CH
cycling, respectively, were positively correlated following rRNA and metatranscriptomic network analysis. The latter phylotype was positively correlated to CH
fluxes (r = 0.46, p < 0.0001). Phylotype functional assignments were supported by metatranscriptomic analysis. We propose that active microbial populations respond primarily to changes in hydrology, pH, and nutrient availability. The increased microbial carbon export observed over winter may have ramifications for climate-soil feedbacks in the PCTR.</description><subject>Carbon Cycle</subject><subject>Carbon Dioxide - analysis</subject><subject>Climate</subject><subject>Gene Expression Profiling</subject><subject>Methane - analysis</subject><subject>Northwestern United States</subject><subject>Rainforest</subject><subject>Seasons</subject><subject>Soil - chemistry</subject><subject>Soil Microbiology</subject><issn>1751-7370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1OwzAUhC0kREvhAGzQO0ADdu3EyRKFnyJV6oLuq-f4pRglcWSnFb0DhyZAWc1ovtEshrEbwe8El_l9VEIWWcJFnnApVSLP2FToVCRaaj5hlzF-cJ7qLNMXbCJ5KvOFzqbs640w-g4bwM4CVf79aINv_M5VYxZot29wcL4DXwNWgzsQtK4K3rgR974_4QiuO_jmQHY08Lgu51CuYQHz39lyCQrqZv9JPz0YqO0p4EAQ0HW1DxQHiN41V-y8xibS9UlnbPP8tCmXyWr98lo-rJK-kENSq0JZmRaGNJdmkauCOFWobIWUoTFGcFRUkEgtoc3TKrO14pgaozI9Ejljt3-z_d60ZLd9cC2G4_b_FfkNxGxlgw</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Levy-Booth, David J</creator><creator>Giesbrecht, Ian J W</creator><creator>Kellogg, Colleen T E</creator><creator>Heger, Thierry J</creator><creator>D'Amore, David V</creator><creator>Keeling, Patrick J</creator><creator>Hallam, Steven J</creator><creator>Mohn, William W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0001-7013-4766</orcidid><orcidid>https://orcid.org/0000-0003-3614-0964</orcidid><orcidid>https://orcid.org/0000-0002-7644-0745</orcidid><orcidid>https://orcid.org/0000-0002-4889-6876</orcidid></search><sort><creationdate>201904</creationdate><title>Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO 2 , and CH 4 fluxes in temperate rainforest soil</title><author>Levy-Booth, David J ; Giesbrecht, Ian J W ; Kellogg, Colleen T E ; Heger, Thierry J ; D'Amore, David V ; Keeling, Patrick J ; Hallam, Steven J ; Mohn, William W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p93t-f494d359be703b2849e0eca4dcae6abbb10a4e9e15dead85c6df40a5bb467a4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Carbon Cycle</topic><topic>Carbon Dioxide - analysis</topic><topic>Climate</topic><topic>Gene Expression Profiling</topic><topic>Methane - analysis</topic><topic>Northwestern United States</topic><topic>Rainforest</topic><topic>Seasons</topic><topic>Soil - chemistry</topic><topic>Soil Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levy-Booth, David J</creatorcontrib><creatorcontrib>Giesbrecht, Ian J W</creatorcontrib><creatorcontrib>Kellogg, Colleen T E</creatorcontrib><creatorcontrib>Heger, Thierry J</creatorcontrib><creatorcontrib>D'Amore, David V</creatorcontrib><creatorcontrib>Keeling, Patrick J</creatorcontrib><creatorcontrib>Hallam, Steven J</creatorcontrib><creatorcontrib>Mohn, William W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>The ISME Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levy-Booth, David J</au><au>Giesbrecht, Ian J W</au><au>Kellogg, Colleen T E</au><au>Heger, Thierry J</au><au>D'Amore, David V</au><au>Keeling, Patrick J</au><au>Hallam, Steven J</au><au>Mohn, William W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO 2 , and CH 4 fluxes in temperate rainforest soil</atitle><jtitle>The ISME Journal</jtitle><addtitle>ISME J</addtitle><date>2019-04</date><risdate>2019</risdate><volume>13</volume><issue>4</issue><spage>950</spage><pages>950-</pages><eissn>1751-7370</eissn><abstract>The Pacific coastal temperate rainforest (PCTR) is a global hot-spot for carbon cycling and export. Yet the influence of microorganisms on carbon cycling processes in PCTR soil is poorly characterized. We developed and tested a conceptual model of seasonal microbial carbon cycling in PCTR soil through integration of geochemistry, micro-meteorology, and eukaryotic and prokaryotic ribosomal amplicon (rRNA) sequencing from 216 soil DNA and RNA libraries. Soil moisture and pH increased during the wet season, with significant correlation to net CO
flux in peat bog and net CH
flux in bog forest soil. Fungal succession in these sites was characterized by the apparent turnover of Archaeorhizomycetes phylotypes accounting for 41% of ITS libraries. Anaerobic prokaryotes, including Syntrophobacteraceae and Methanomicrobia increased in rRNA libraries during the wet season. Putatively active populations of these phylotypes and their biogeochemical marker genes for sulfate and CH
cycling, respectively, were positively correlated following rRNA and metatranscriptomic network analysis. The latter phylotype was positively correlated to CH
fluxes (r = 0.46, p < 0.0001). Phylotype functional assignments were supported by metatranscriptomic analysis. We propose that active microbial populations respond primarily to changes in hydrology, pH, and nutrient availability. The increased microbial carbon export observed over winter may have ramifications for climate-soil feedbacks in the PCTR.</abstract><cop>England</cop><pmid>30538276</pmid><doi>10.1038/s41396-018-0334-3</doi><orcidid>https://orcid.org/0000-0001-7013-4766</orcidid><orcidid>https://orcid.org/0000-0003-3614-0964</orcidid><orcidid>https://orcid.org/0000-0002-7644-0745</orcidid><orcidid>https://orcid.org/0000-0002-4889-6876</orcidid></addata></record> |
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| source | Oxford Journals Open Access Collection; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Carbon Cycle Carbon Dioxide - analysis Climate Gene Expression Profiling Methane - analysis Northwestern United States Rainforest Seasons Soil - chemistry Soil Microbiology |
| title | Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO 2 , and CH 4 fluxes in temperate rainforest soil |
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