Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs

Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to d...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2018-11, Vol.54 (9), p.12734-12737
Hauptverfasser:	Fukunaga, Keisuke, Watanabe, Takayoshi, Novitasari, Dian, Ohashi, Hiroyuki, Abe, Ryoji, Hohsaka, Takahiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Fluorescence Monoclonal antibodies Peptides Reductive alkylation
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creator	Fukunaga, Keisuke Watanabe, Takayoshi Novitasari, Dian Ohashi, Hiroyuki Abe, Ryoji Hohsaka, Takahiro
description	Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to detect antigens in a rapid and quantitative manner. Fluorescent antibody probes showing antigen-dependent fluorescence responses were developed by N-terminal-selective reductive alkylation of IgGs.
doi_str_mv	10.1039/c8cc07827k
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source	Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects	Antigens Fluorescence Monoclonal antibodies Peptides Reductive alkylation
title	Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs
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