Recent progress in enzymatic protein labelling techniques and their applications

Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly...

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Veröffentlicht in:Chemical Society reviews 2018-12, Vol.47 (24), p.916-9136
Hauptverfasser: Zhang, Yi, Park, Keun-Young, Suazo, Kiall F, Distefano, Mark D
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Park, Keun-Young
Suazo, Kiall F
Distefano, Mark D
description Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly enhances their pharmacokinetics or potency. To label a protein of interest, conventional direct chemical reaction with the side-chains of native amino acids often yields heterogeneously modified products. This renders their characterization complicated, requires difficult separation steps and may impact protein function. Although modification can also be achieved via the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase, N -myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme. This review describes recent progress in employing enzymatic labelling techniques to modify proteins for a diverse range of applications.
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Amino acids
Animals
Biocatalysis
biopharmaceuticals
Biotin
Chemical compounds
Chemical reactions
Chemistry Techniques, Synthetic - methods
Conjugates
Conjugation
cytotoxicity
cytotoxins
enzymatic treatment
Fluorescence
fluorescent dyes
Functional groups
geranylgeranyl diphosphate synthase
Humans
image analysis
Labeling
labeling techniques
Lipoic acid
Microorganisms
Models, Molecular
moieties
Organic chemistry
pharmacokinetics
Pharmacology
Polymers
protein synthesis
protein-glutamine gamma-glutamyltransferase
protein-protein interactions
Proteins
Proteins - analysis
Proteins - chemical synthesis
Proteins - metabolism
sortase A
Staining and Labeling - methods
tubulin
title Recent progress in enzymatic protein labelling techniques and their applications
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