Recent progress in enzymatic protein labelling techniques and their applications
Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly...
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description | Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly enhances their pharmacokinetics or potency. To label a protein of interest, conventional direct chemical reaction with the side-chains of native amino acids often yields heterogeneously modified products. This renders their characterization complicated, requires difficult separation steps and may impact protein function. Although modification can also be achieved
via
the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase,
N
-myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme.
This review describes recent progress in employing enzymatic labelling techniques to modify proteins for a diverse range of applications. |
doi_str_mv | 10.1039/c8cs00537k |
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via
the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase,
N
-myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme.
This review describes recent progress in employing enzymatic labelling techniques to modify proteins for a diverse range of applications.</description><identifier>ISSN: 0306-0012</identifier><identifier>ISSN: 1460-4744</identifier><identifier>EISSN: 1460-4744</identifier><identifier>DOI: 10.1039/c8cs00537k</identifier><identifier>PMID: 30259933</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Amino acids ; Animals ; Biocatalysis ; biopharmaceuticals ; Biotin ; Chemical compounds ; Chemical reactions ; Chemistry Techniques, Synthetic - methods ; Conjugates ; Conjugation ; cytotoxicity ; cytotoxins ; enzymatic treatment ; Fluorescence ; fluorescent dyes ; Functional groups ; geranylgeranyl diphosphate synthase ; Humans ; image analysis ; Labeling ; labeling techniques ; Lipoic acid ; Microorganisms ; Models, Molecular ; moieties ; Organic chemistry ; pharmacokinetics ; Pharmacology ; Polymers ; protein synthesis ; protein-glutamine gamma-glutamyltransferase ; protein-protein interactions ; Proteins ; Proteins - analysis ; Proteins - chemical synthesis ; Proteins - metabolism ; sortase A ; Staining and Labeling - methods ; tubulin</subject><ispartof>Chemical Society reviews, 2018-12, Vol.47 (24), p.916-9136</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-b8eadf26f4cf796cb2f9dd0e29f69d80a67ea411c8c7364d559554799167b98c3</citedby><cites>FETCH-LOGICAL-c502t-b8eadf26f4cf796cb2f9dd0e29f69d80a67ea411c8c7364d559554799167b98c3</cites><orcidid>0000-0002-2872-0259 ; 0000-0002-4398-3065 ; 0000-0002-0803-8332 ; 0000-0002-5474-5812</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30259933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Park, Keun-Young</creatorcontrib><creatorcontrib>Suazo, Kiall F</creatorcontrib><creatorcontrib>Distefano, Mark D</creatorcontrib><title>Recent progress in enzymatic protein labelling techniques and their applications</title><title>Chemical Society reviews</title><addtitle>Chem Soc Rev</addtitle><description>Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly enhances their pharmacokinetics or potency. To label a protein of interest, conventional direct chemical reaction with the side-chains of native amino acids often yields heterogeneously modified products. This renders their characterization complicated, requires difficult separation steps and may impact protein function. Although modification can also be achieved
via
the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase,
N
-myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme.
This review describes recent progress in employing enzymatic labelling techniques to modify proteins for a diverse range of applications.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Biocatalysis</subject><subject>biopharmaceuticals</subject><subject>Biotin</subject><subject>Chemical compounds</subject><subject>Chemical reactions</subject><subject>Chemistry Techniques, Synthetic - methods</subject><subject>Conjugates</subject><subject>Conjugation</subject><subject>cytotoxicity</subject><subject>cytotoxins</subject><subject>enzymatic treatment</subject><subject>Fluorescence</subject><subject>fluorescent dyes</subject><subject>Functional groups</subject><subject>geranylgeranyl diphosphate synthase</subject><subject>Humans</subject><subject>image analysis</subject><subject>Labeling</subject><subject>labeling techniques</subject><subject>Lipoic acid</subject><subject>Microorganisms</subject><subject>Models, Molecular</subject><subject>moieties</subject><subject>Organic chemistry</subject><subject>pharmacokinetics</subject><subject>Pharmacology</subject><subject>Polymers</subject><subject>protein synthesis</subject><subject>protein-glutamine gamma-glutamyltransferase</subject><subject>protein-protein interactions</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Proteins - chemical synthesis</subject><subject>Proteins - metabolism</subject><subject>sortase A</subject><subject>Staining and Labeling - methods</subject><subject>tubulin</subject><issn>0306-0012</issn><issn>1460-4744</issn><issn>1460-4744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtLAzEUhYMoWqsb98qAGxFG857JRpDiCwuKj3XIZDJtdJoZk6lQf72prfWRzQ33fvdwDweAPQRPECTiVOc6QMhI9roGeohymNKM0nXQgwTyFEKEt8B2CC_xhzKON8EWgZgJQUgP3D8YbVyXtL4ZeRNCYl1i3Mdsojqr593OxE6tClPX1o2Szuixs29TExLlyqQbG-sT1ba11XGjcWEHbFSqDmZ3Wfvg-fLiaXCdDu-ubgbnw1QziLu0yI0qK8wrqqtMcF3gSpQlNFhUXJQ5VDwziiIUvWWE05IxwRjNhEA8K0SuSR-cLXTbaTEx5dyEV7VsvZ0oP5ONsvLvxNmxHDXvkuNccIKiwNFSwDdzP52c2KCjTeVMMw0SY4wgxJjQiB7-Q1-aqXfRnsSIEc5QfJE6XlDaNyF4U62OQVDOg5KDfPD4FdRthA9-n79Cv5OJwP4C8EGvpj9Jk09gaZlO</recordid><startdate>20181221</startdate><enddate>20181221</enddate><creator>Zhang, Yi</creator><creator>Park, Keun-Young</creator><creator>Suazo, Kiall F</creator><creator>Distefano, Mark D</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2872-0259</orcidid><orcidid>https://orcid.org/0000-0002-4398-3065</orcidid><orcidid>https://orcid.org/0000-0002-0803-8332</orcidid><orcidid>https://orcid.org/0000-0002-5474-5812</orcidid></search><sort><creationdate>20181221</creationdate><title>Recent progress in enzymatic protein labelling techniques and their applications</title><author>Zhang, Yi ; Park, Keun-Young ; Suazo, Kiall F ; Distefano, Mark D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-b8eadf26f4cf796cb2f9dd0e29f69d80a67ea411c8c7364d559554799167b98c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Biocatalysis</topic><topic>biopharmaceuticals</topic><topic>Biotin</topic><topic>Chemical compounds</topic><topic>Chemical reactions</topic><topic>Chemistry Techniques, Synthetic - methods</topic><topic>Conjugates</topic><topic>Conjugation</topic><topic>cytotoxicity</topic><topic>cytotoxins</topic><topic>enzymatic treatment</topic><topic>Fluorescence</topic><topic>fluorescent dyes</topic><topic>Functional groups</topic><topic>geranylgeranyl diphosphate synthase</topic><topic>Humans</topic><topic>image analysis</topic><topic>Labeling</topic><topic>labeling techniques</topic><topic>Lipoic acid</topic><topic>Microorganisms</topic><topic>Models, Molecular</topic><topic>moieties</topic><topic>Organic chemistry</topic><topic>pharmacokinetics</topic><topic>Pharmacology</topic><topic>Polymers</topic><topic>protein synthesis</topic><topic>protein-glutamine gamma-glutamyltransferase</topic><topic>protein-protein interactions</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteins - chemical synthesis</topic><topic>Proteins - metabolism</topic><topic>sortase A</topic><topic>Staining and Labeling - methods</topic><topic>tubulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Park, Keun-Young</creatorcontrib><creatorcontrib>Suazo, Kiall F</creatorcontrib><creatorcontrib>Distefano, Mark D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical Society reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yi</au><au>Park, Keun-Young</au><au>Suazo, Kiall F</au><au>Distefano, Mark D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recent progress in enzymatic protein labelling techniques and their applications</atitle><jtitle>Chemical Society reviews</jtitle><addtitle>Chem Soc Rev</addtitle><date>2018-12-21</date><risdate>2018</risdate><volume>47</volume><issue>24</issue><spage>916</spage><epage>9136</epage><pages>916-9136</pages><issn>0306-0012</issn><issn>1460-4744</issn><eissn>1460-4744</eissn><abstract>Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly enhances their pharmacokinetics or potency. To label a protein of interest, conventional direct chemical reaction with the side-chains of native amino acids often yields heterogeneously modified products. This renders their characterization complicated, requires difficult separation steps and may impact protein function. Although modification can also be achieved
via
the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase,
N
-myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme.
This review describes recent progress in employing enzymatic labelling techniques to modify proteins for a diverse range of applications.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>30259933</pmid><doi>10.1039/c8cs00537k</doi><tpages>31</tpages><orcidid>https://orcid.org/0000-0002-2872-0259</orcidid><orcidid>https://orcid.org/0000-0002-4398-3065</orcidid><orcidid>https://orcid.org/0000-0002-0803-8332</orcidid><orcidid>https://orcid.org/0000-0002-5474-5812</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Amino acids Animals Biocatalysis biopharmaceuticals Biotin Chemical compounds Chemical reactions Chemistry Techniques, Synthetic - methods Conjugates Conjugation cytotoxicity cytotoxins enzymatic treatment Fluorescence fluorescent dyes Functional groups geranylgeranyl diphosphate synthase Humans image analysis Labeling labeling techniques Lipoic acid Microorganisms Models, Molecular moieties Organic chemistry pharmacokinetics Pharmacology Polymers protein synthesis protein-glutamine gamma-glutamyltransferase protein-protein interactions Proteins Proteins - analysis Proteins - chemical synthesis Proteins - metabolism sortase A Staining and Labeling - methods tubulin |
title | Recent progress in enzymatic protein labelling techniques and their applications |
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