Taurine activates glycine and GABA A receptor currents in anoxia-tolerant painted turtle pyramidal neurons
Unlike anoxia-intolerant mammals, painted turtles can survive extended periods without oxygen. This is partly accomplished by an anoxia-mediated increase in gamma-aminobutyric acid (GABA) release, which activates GABA receptors and mediates spike arrest in turtle neurons via shunting inhibition. Ext...
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creator | Miles, Ashley Rebecca Hawrysh, Peter John Hossein-Javaheri, Nariman Buck, Leslie Thomas |
description | Unlike anoxia-intolerant mammals, painted turtles can survive extended periods without oxygen. This is partly accomplished by an anoxia-mediated increase in gamma-aminobutyric acid (GABA) release, which activates GABA receptors and mediates spike arrest in turtle neurons via shunting inhibition. Extracellular taurine levels also increase during anoxia; however, its function is unknown but speculated to involve glycine and/or GABA
receptors. Given the general importance of inhibitory neurotransmission in the anoxia-tolerant painted turtle brain, we investigated the function of taurine as an inhibitory neuromodulator in turtle pyramidal neurons. Using whole-cell patch-clamp electrophysiological methods to record from neurons within a cortical brain sheet, we found that taurine depolarized membrane potential by approximately 8 mV, increased whole cell conductance by approximately 2-fold, and induced an inward current that possessed characteristics similar to GABA- and glycine-evoked currents. These effects were mitigated following glycine receptor antagonism with strychnine and GABA
receptor antagonism with gabazine, bicuculine, or picrotoxin, but were unchanged following GABA
or glutamatergic receptor inhibition. These data indicate that high concentrations of taurine in vitro mediates its effects through both glycine and GABA
receptors, and suggest that taurine, in addition to GABA, inhibits neuronal activity during anoxia in the turtle cortex. |
doi_str_mv | 10.1242/jeb.181529 |
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receptors. Given the general importance of inhibitory neurotransmission in the anoxia-tolerant painted turtle brain, we investigated the function of taurine as an inhibitory neuromodulator in turtle pyramidal neurons. Using whole-cell patch-clamp electrophysiological methods to record from neurons within a cortical brain sheet, we found that taurine depolarized membrane potential by approximately 8 mV, increased whole cell conductance by approximately 2-fold, and induced an inward current that possessed characteristics similar to GABA- and glycine-evoked currents. These effects were mitigated following glycine receptor antagonism with strychnine and GABA
receptor antagonism with gabazine, bicuculine, or picrotoxin, but were unchanged following GABA
or glutamatergic receptor inhibition. These data indicate that high concentrations of taurine in vitro mediates its effects through both glycine and GABA
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receptors. Given the general importance of inhibitory neurotransmission in the anoxia-tolerant painted turtle brain, we investigated the function of taurine as an inhibitory neuromodulator in turtle pyramidal neurons. Using whole-cell patch-clamp electrophysiological methods to record from neurons within a cortical brain sheet, we found that taurine depolarized membrane potential by approximately 8 mV, increased whole cell conductance by approximately 2-fold, and induced an inward current that possessed characteristics similar to GABA- and glycine-evoked currents. These effects were mitigated following glycine receptor antagonism with strychnine and GABA
receptor antagonism with gabazine, bicuculine, or picrotoxin, but were unchanged following GABA
or glutamatergic receptor inhibition. These data indicate that high concentrations of taurine in vitro mediates its effects through both glycine and GABA
receptors, and suggest that taurine, in addition to GABA, inhibits neuronal activity during anoxia in the turtle cortex.</description><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo1j81KAzEUhYMgtlY3PoDkBabOTTI_WY5Fq1BwU9flJrkjKdPMkMmI8_YWf87mwHfgg8PYHeRrEEo8HMmsoYZC6Au2BFVVmQZVLNj1OB7zc8pCXbGFzIWshIIlO-5xij4QR5v8JyYa-Uc32x8SHN82jw1veCRLQ-ojt1OMFNLIfTjv_ZfHLPUdRQyJD-hDIsfTFFNHfJgjnrzDjgeaYh_GG3bZYjfS7V-v2Pvz037zku3etq-bZpcNkNcpg1YbVdfooLagQApyJdmyaBGNNkJYBZqqSrToTFtaKXQl61I7dFo7gyRX7P7XO0zmRO4wRH_COB_-T8tvG8VZKw</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Miles, Ashley Rebecca</creator><creator>Hawrysh, Peter John</creator><creator>Hossein-Javaheri, Nariman</creator><creator>Buck, Leslie Thomas</creator><scope>NPM</scope><orcidid>https://orcid.org/0000-0002-2637-7821</orcidid></search><sort><creationdate>20181101</creationdate><title>Taurine activates glycine and GABA A receptor currents in anoxia-tolerant painted turtle pyramidal neurons</title><author>Miles, Ashley Rebecca ; Hawrysh, Peter John ; Hossein-Javaheri, Nariman ; Buck, Leslie Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-1f9b488ad18c14132ed6ec65faab9b22c419e772fadbf6c32973869dad99dbae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miles, Ashley Rebecca</creatorcontrib><creatorcontrib>Hawrysh, Peter John</creatorcontrib><creatorcontrib>Hossein-Javaheri, Nariman</creatorcontrib><creatorcontrib>Buck, Leslie Thomas</creatorcontrib><collection>PubMed</collection><jtitle>Journal of experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miles, Ashley Rebecca</au><au>Hawrysh, Peter John</au><au>Hossein-Javaheri, Nariman</au><au>Buck, Leslie Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Taurine activates glycine and GABA A receptor currents in anoxia-tolerant painted turtle pyramidal neurons</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>2018-11-01</date><risdate>2018</risdate><eissn>1477-9145</eissn><abstract>Unlike anoxia-intolerant mammals, painted turtles can survive extended periods without oxygen. This is partly accomplished by an anoxia-mediated increase in gamma-aminobutyric acid (GABA) release, which activates GABA receptors and mediates spike arrest in turtle neurons via shunting inhibition. Extracellular taurine levels also increase during anoxia; however, its function is unknown but speculated to involve glycine and/or GABA
receptors. Given the general importance of inhibitory neurotransmission in the anoxia-tolerant painted turtle brain, we investigated the function of taurine as an inhibitory neuromodulator in turtle pyramidal neurons. Using whole-cell patch-clamp electrophysiological methods to record from neurons within a cortical brain sheet, we found that taurine depolarized membrane potential by approximately 8 mV, increased whole cell conductance by approximately 2-fold, and induced an inward current that possessed characteristics similar to GABA- and glycine-evoked currents. These effects were mitigated following glycine receptor antagonism with strychnine and GABA
receptor antagonism with gabazine, bicuculine, or picrotoxin, but were unchanged following GABA
or glutamatergic receptor inhibition. These data indicate that high concentrations of taurine in vitro mediates its effects through both glycine and GABA
receptors, and suggest that taurine, in addition to GABA, inhibits neuronal activity during anoxia in the turtle cortex.</abstract><cop>England</cop><pmid>30237241</pmid><doi>10.1242/jeb.181529</doi><orcidid>https://orcid.org/0000-0002-2637-7821</orcidid><oa>free_for_read</oa></addata></record> |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
title | Taurine activates glycine and GABA A receptor currents in anoxia-tolerant painted turtle pyramidal neurons |
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