Identification and Purification of a Bacterial Ice-Nucleation Protein
The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1986-10, Vol.83 (19), p.7256-7260 |
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| container_issue | 19 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Wolber, Paul K. Deininger, Caroline A. Southworth, Maurice W. Vandekerckhove, Joel Van Montagu, Marc Warren, Gareth J. |
| description | The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl β -D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process. |
| doi_str_mv | 10.1073/pnas.83.19.7256 |
| format | Article |
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The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl β -D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.83.19.7256</identifier><identifier>PMID: 3020542</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Amino Acids - analysis ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacteriology ; Biological and medical sciences ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene Expression Regulation ; Genes, Bacterial ; Ice ; Ice nuclei ; Lipids ; Macromolecular Substances ; Membrane Proteins - genetics ; Membrane Proteins - isolation & purification ; Microbiology ; Molecular Weight ; Molecules ; Morphology, structure, chemical composition ; Nuclear membrane ; Nucleation ; Proteins ; Pseudomonas - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1986-10, Vol.83 (19), p.7256-7260</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-8692e5d59a22583eb3e62a809386daa61cf85913dd236cf35002405eaa9381ae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/83/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/28449$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/28449$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27929,27930,53796,53798,58022,58255</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7872720$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3020542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolber, Paul K.</creatorcontrib><creatorcontrib>Deininger, Caroline A.</creatorcontrib><creatorcontrib>Southworth, Maurice W.</creatorcontrib><creatorcontrib>Vandekerckhove, Joel</creatorcontrib><creatorcontrib>Van Montagu, Marc</creatorcontrib><creatorcontrib>Warren, Gareth J.</creatorcontrib><title>Identification and Purification of a Bacterial Ice-Nucleation Protein</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl β -D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process.</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene Expression Regulation</subject><subject>Genes, Bacterial</subject><subject>Ice</subject><subject>Ice nuclei</subject><subject>Lipids</subject><subject>Macromolecular Substances</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Molecules</subject><subject>Morphology, structure, chemical composition</subject><subject>Nuclear membrane</subject><subject>Nucleation</subject><subject>Proteins</subject><subject>Pseudomonas - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1vFDEYhC0ECkegRkICbYGg2ou_PwoKiAKcFEEKqK03Xi848q0vthfBv8enWy7QUFnyPPPOaBB6SvCaYMXOdhOUtWZrYtaKCnkPrQg2pJfc4PtohTFVveaUP0SPSrnBGBuh8Qk6YZhiwekKXWwGP9UwBgc1pKmDaeiu5nz3kcYOunfgqs8BYrdxvv80u-gP6lVO1YfpMXowQiz-yfKeoq_vL76cf-wvP3_YnL-97J0QsvZaGurFIAxQKjTz18xLChobpuUAIIkbtTCEDQNl0o1MtP4cCw_QCAKenaI3h7u7-XrrB9eqZ4h2l8MW8i-bINh_lSl8t9_SD9sCpOHN_2rx53Q7-1LtNhTnY4TJp7lYpbDUnKgGnh1Al1Mp2Y_HDILtfni7H95qZomx--Gb4_nf1Y78snTTXy46FAdxzDC5UI6Y0ooqihv2esH29_-odzl2nGOs_mdt5Iv_kg14dgBuSk35SFDNuWG_AQ51rP0</recordid><startdate>19861001</startdate><enddate>19861001</enddate><creator>Wolber, Paul K.</creator><creator>Deininger, Caroline A.</creator><creator>Southworth, Maurice W.</creator><creator>Vandekerckhove, Joel</creator><creator>Van Montagu, Marc</creator><creator>Warren, Gareth J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19861001</creationdate><title>Identification and Purification of a Bacterial Ice-Nucleation Protein</title><author>Wolber, Paul K. ; Deininger, Caroline A. ; Southworth, Maurice W. ; Vandekerckhove, Joel ; Van Montagu, Marc ; Warren, Gareth J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-8692e5d59a22583eb3e62a809386daa61cf85913dd236cf35002405eaa9381ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Gene Expression Regulation</topic><topic>Genes, Bacterial</topic><topic>Ice</topic><topic>Ice nuclei</topic><topic>Lipids</topic><topic>Macromolecular Substances</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Molecules</topic><topic>Morphology, structure, chemical composition</topic><topic>Nuclear membrane</topic><topic>Nucleation</topic><topic>Proteins</topic><topic>Pseudomonas - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolber, Paul K.</creatorcontrib><creatorcontrib>Deininger, Caroline A.</creatorcontrib><creatorcontrib>Southworth, Maurice W.</creatorcontrib><creatorcontrib>Vandekerckhove, Joel</creatorcontrib><creatorcontrib>Van Montagu, Marc</creatorcontrib><creatorcontrib>Warren, Gareth J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolber, Paul K.</au><au>Deininger, Caroline A.</au><au>Southworth, Maurice W.</au><au>Vandekerckhove, Joel</au><au>Van Montagu, Marc</au><au>Warren, Gareth J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Purification of a Bacterial Ice-Nucleation Protein</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1986-10-01</date><risdate>1986</risdate><volume>83</volume><issue>19</issue><spage>7256</spage><epage>7260</epage><pages>7256-7260</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl β -D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3020542</pmid><doi>10.1073/pnas.83.19.7256</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1986-10, Vol.83 (19), p.7256-7260 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Amino Acids - analysis Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacteriology Biological and medical sciences Cloning, Molecular Fundamental and applied biological sciences. Psychology Gels Gene Expression Regulation Genes, Bacterial Ice Ice nuclei Lipids Macromolecular Substances Membrane Proteins - genetics Membrane Proteins - isolation & purification Microbiology Molecular Weight Molecules Morphology, structure, chemical composition Nuclear membrane Nucleation Proteins Pseudomonas - genetics |
| title | Identification and Purification of a Bacterial Ice-Nucleation Protein |
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