A novel electrochemical aptasensor for detection of aflatoxin M 1 based on target-induced immobilization of gold nanoparticles on the surface of electrode
In this study, a novel electrochemical aptasensor was developed for detection of AFM aflatoxin M (AFM ) based on the hairpin-shaped structure of AFM aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the pr...
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| Veröffentlicht in: | Biosensors & bioelectronics 2018-10, Vol.117, p.487 |
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| creator | Jalalian, Seyed Hamid Ramezani, Mohammad Danesh, Noor Mohammad Alibolandi, Mona Abnous, Khalil Taghdisi, Seyed Mohammad |
| description | In this study, a novel electrochemical aptasensor was developed for detection of AFM
aflatoxin M
(AFM
) based on the hairpin-shaped structure of AFM
aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM
and also negatively charged AuNPs allowed detection of AFM
with high sensitivity and selectivity. In the absence of the AFM
, the hairpin structure of the Apt was intact. So, a weak peak current was obtained. However, addition of AFM
could disassemble the hairpin structure of the Apt. Thus, the CS-modified AuNPs came to close proximity of the surface of electrode and a strong current signal was recorded upon the addition of methylene blue as redox agent. The aptasensor allowed determination of AFM
with a detection limit of 0.9 ng/L. Finally, the aptasensor was successfully applied for detection of AFM
in real samples, including milk and serum samples. |
| doi_str_mv | 10.1016/j.bios.2018.06.055 |
| format | Article |
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aflatoxin M
(AFM
) based on the hairpin-shaped structure of AFM
aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM
and also negatively charged AuNPs allowed detection of AFM
with high sensitivity and selectivity. In the absence of the AFM
, the hairpin structure of the Apt was intact. So, a weak peak current was obtained. However, addition of AFM
could disassemble the hairpin structure of the Apt. Thus, the CS-modified AuNPs came to close proximity of the surface of electrode and a strong current signal was recorded upon the addition of methylene blue as redox agent. The aptasensor allowed determination of AFM
with a detection limit of 0.9 ng/L. Finally, the aptasensor was successfully applied for detection of AFM
in real samples, including milk and serum samples.</description><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2018.06.055</identifier><identifier>PMID: 29982118</identifier><language>eng</language><publisher>England</publisher><subject>Aflatoxin M1 - analysis ; Aflatoxin M1 - blood ; Animals ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Electrochemical Techniques ; Electrodes ; Gold - chemistry ; Limit of Detection ; Metal Nanoparticles - chemistry ; Milk - chemistry ; Serum - chemistry</subject><ispartof>Biosensors & bioelectronics, 2018-10, Vol.117, p.487</ispartof><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29982118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jalalian, Seyed Hamid</creatorcontrib><creatorcontrib>Ramezani, Mohammad</creatorcontrib><creatorcontrib>Danesh, Noor Mohammad</creatorcontrib><creatorcontrib>Alibolandi, Mona</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Taghdisi, Seyed Mohammad</creatorcontrib><title>A novel electrochemical aptasensor for detection of aflatoxin M 1 based on target-induced immobilization of gold nanoparticles on the surface of electrode</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>In this study, a novel electrochemical aptasensor was developed for detection of AFM
aflatoxin M
(AFM
) based on the hairpin-shaped structure of AFM
aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM
and also negatively charged AuNPs allowed detection of AFM
with high sensitivity and selectivity. In the absence of the AFM
, the hairpin structure of the Apt was intact. So, a weak peak current was obtained. However, addition of AFM
could disassemble the hairpin structure of the Apt. Thus, the CS-modified AuNPs came to close proximity of the surface of electrode and a strong current signal was recorded upon the addition of methylene blue as redox agent. The aptasensor allowed determination of AFM
with a detection limit of 0.9 ng/L. Finally, the aptasensor was successfully applied for detection of AFM
in real samples, including milk and serum samples.</description><subject>Aflatoxin M1 - analysis</subject><subject>Aflatoxin M1 - blood</subject><subject>Animals</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Electrochemical Techniques</subject><subject>Electrodes</subject><subject>Gold - chemistry</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Milk - chemistry</subject><subject>Serum - chemistry</subject><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UEtOwzAUtJAQLYULsEC-QMKzHTvJsqr4SSA2sK4c-7l1lcRR7CLgKJyWAO3iaaQ3H42GkCsGOQOmbnZ540PMObAqB5WDlCdkzqpSZAUXckbOY9wBQMlqOCMzXtcVZ6yak-8l7cM7thRbNGkMZoudN7qlekg6Yh_DSN10FtPE-9DT4Kh2rU7hw_f0mTLaTDpLJybpcYMp873dm-njuy40vvVf-ujbhNbSXvdh0GPypsX4Z9sijfvRaYO_okMRixfk1Ok24uUBF-Tt7vZ19ZA9vdw_rpZP2cCgSpkCjlo6hZVrGqM01s4oXgATotJQFxJRTFiCUGBBNUbYkotCcitKKaQQC3L9nzvsmw7tehh9p8fP9XEk8QOVc2uD</recordid><startdate>20181015</startdate><enddate>20181015</enddate><creator>Jalalian, Seyed Hamid</creator><creator>Ramezani, Mohammad</creator><creator>Danesh, Noor Mohammad</creator><creator>Alibolandi, Mona</creator><creator>Abnous, Khalil</creator><creator>Taghdisi, Seyed Mohammad</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20181015</creationdate><title>A novel electrochemical aptasensor for detection of aflatoxin M 1 based on target-induced immobilization of gold nanoparticles on the surface of electrode</title><author>Jalalian, Seyed Hamid ; Ramezani, Mohammad ; Danesh, Noor Mohammad ; Alibolandi, Mona ; Abnous, Khalil ; Taghdisi, Seyed Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-602ea5f6e8fbbc6ae9fc62401338a0945ee3a0970360d06bc3d723452d3753533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aflatoxin M1 - analysis</topic><topic>Aflatoxin M1 - blood</topic><topic>Animals</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Electrochemical Techniques</topic><topic>Electrodes</topic><topic>Gold - chemistry</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Milk - chemistry</topic><topic>Serum - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jalalian, Seyed Hamid</creatorcontrib><creatorcontrib>Ramezani, Mohammad</creatorcontrib><creatorcontrib>Danesh, Noor Mohammad</creatorcontrib><creatorcontrib>Alibolandi, Mona</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Taghdisi, Seyed Mohammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jalalian, Seyed Hamid</au><au>Ramezani, Mohammad</au><au>Danesh, Noor Mohammad</au><au>Alibolandi, Mona</au><au>Abnous, Khalil</au><au>Taghdisi, Seyed Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel electrochemical aptasensor for detection of aflatoxin M 1 based on target-induced immobilization of gold nanoparticles on the surface of electrode</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2018-10-15</date><risdate>2018</risdate><volume>117</volume><spage>487</spage><pages>487-</pages><eissn>1873-4235</eissn><abstract>In this study, a novel electrochemical aptasensor was developed for detection of AFM
aflatoxin M
(AFM
) based on the hairpin-shaped structure of AFM
aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM
and also negatively charged AuNPs allowed detection of AFM
with high sensitivity and selectivity. In the absence of the AFM
, the hairpin structure of the Apt was intact. So, a weak peak current was obtained. However, addition of AFM
could disassemble the hairpin structure of the Apt. Thus, the CS-modified AuNPs came to close proximity of the surface of electrode and a strong current signal was recorded upon the addition of methylene blue as redox agent. The aptasensor allowed determination of AFM
with a detection limit of 0.9 ng/L. Finally, the aptasensor was successfully applied for detection of AFM
in real samples, including milk and serum samples.</abstract><cop>England</cop><pmid>29982118</pmid><doi>10.1016/j.bios.2018.06.055</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1873-4235 |
| ispartof | Biosensors & bioelectronics, 2018-10, Vol.117, p.487 |
| issn | 1873-4235 |
| language | eng |
| recordid | cdi_pubmed_primary_29982118 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aflatoxin M1 - analysis Aflatoxin M1 - blood Animals Biosensing Techniques - instrumentation Biosensing Techniques - methods Electrochemical Techniques Electrodes Gold - chemistry Limit of Detection Metal Nanoparticles - chemistry Milk - chemistry Serum - chemistry |
| title | A novel electrochemical aptasensor for detection of aflatoxin M 1 based on target-induced immobilization of gold nanoparticles on the surface of electrode |
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