Tandemly Repeated Nonribosomal DNA Sequences in the Chloroplast Genome of an Acetabularia mediterranea Strain
A purified chloroplast fraction was prepared from caps of the giant unicellular green alga Acetabularia mediterranea (strain 17). High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is p...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-03, Vol.82 (6), p.1706-1710 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Tymms, Martin J. Schweiger, Hans-Georg |
| description | A purified chloroplast fraction was prepared from caps of the giant unicellular green alga Acetabularia mediterranea (strain 17). High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species Acetabularia crenulata or chloroplast DNA from spinach. |
| doi_str_mv | 10.1073/pnas.82.6.1706 |
| format | Article |
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High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species Acetabularia crenulata or chloroplast DNA from spinach.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.6.1706</identifier><identifier>PMID: 2984664</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Acetabularia - genetics ; Acetabularia crenulata ; Biological and medical sciences ; Cell structures and functions ; Chlorophyta - genetics ; Chloroplast DNA ; Chloroplast, photosynthetic membrane and photosynthesis ; Chloroplasts ; Chloroplasts - metabolism ; Cloning, Molecular ; DNA ; DNA - genetics ; DNA Restriction Enzymes ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gels ; Genetic hybridization ; Genomes ; Molecular and cellular biology ; Molecular weight ; Repetitive Sequences, Nucleic Acid ; rRNA genes ; Sodium</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (6), p.1706-1710</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-77ba0f6514df8946839987ec224421e2cebe8dbf6f912ab54f71cfde1d2cee5b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25487$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25487$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9282954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2984664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tymms, Martin J.</creatorcontrib><creatorcontrib>Schweiger, Hans-Georg</creatorcontrib><title>Tandemly Repeated Nonribosomal DNA Sequences in the Chloroplast Genome of an Acetabularia mediterranea Strain</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A purified chloroplast fraction was prepared from caps of the giant unicellular green alga Acetabularia mediterranea (strain 17). High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species Acetabularia crenulata or chloroplast DNA from spinach.</description><subject>Acetabularia - genetics</subject><subject>Acetabularia crenulata</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Chlorophyta - genetics</subject><subject>Chloroplast DNA</subject><subject>Chloroplast, photosynthetic membrane and photosynthesis</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA Restriction Enzymes</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetic hybridization</subject><subject>Genomes</subject><subject>Molecular and cellular biology</subject><subject>Molecular weight</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>rRNA genes</subject><subject>Sodium</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxVcIVELhygEJyYeqtwTb6_XHgUMUoCBVRaLlbHm9Y7KV1w62g-h_j6OEEC5w8uH9ZuY9v6Z5SfCCYNG-2QSTF5Iu-IIIzB81M4IVmXOm8ONmhjEVc8koe9o8y_keY6w6ic-aM6ok45zNmunOhAEm_4C-wAZMgQHdxJDGPuY4GY_e3SzRLXzfQrCQ0RhQWQNarX1MceNNLugKQpwARYdMQEsLxfRbb9Jo0ATDWCAlE8Cg25LMGJ43T5zxGV4c3vPm64f3d6uP8-vPV59Wy-u57QgvcyF6gx3vCBucVIzLVikpwFLKGCVALfQgh95xpwg1fcecINYNQIYqQde3583b_d7Ntq82LIR63utNGieTHnQ0o_5bCeNaf4s_dKtEy0idvzzMp1iz56KnMVvwvmaJ26wFx13HsfwvSBgjvBOsgos9aFPMOYE7miFY74rUuyK1pJrrXZF14PVphCN-aK7qFwfdZGu8q99sx3zEFJVUdexkzW79b_X0zOW_dO223hf4WSr4ag_e5xLTHzsdk6L9BVlJya8</recordid><startdate>19850301</startdate><enddate>19850301</enddate><creator>Tymms, Martin J.</creator><creator>Schweiger, Hans-Georg</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850301</creationdate><title>Tandemly Repeated Nonribosomal DNA Sequences in the Chloroplast Genome of an Acetabularia mediterranea Strain</title><author>Tymms, Martin J. ; Schweiger, Hans-Georg</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-77ba0f6514df8946839987ec224421e2cebe8dbf6f912ab54f71cfde1d2cee5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acetabularia - genetics</topic><topic>Acetabularia crenulata</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Chlorophyta - genetics</topic><topic>Chloroplast DNA</topic><topic>Chloroplast, photosynthetic membrane and photosynthesis</topic><topic>Chloroplasts</topic><topic>Chloroplasts - metabolism</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA Restriction Enzymes</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genetic hybridization</topic><topic>Genomes</topic><topic>Molecular and cellular biology</topic><topic>Molecular weight</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>rRNA genes</topic><topic>Sodium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tymms, Martin J.</creatorcontrib><creatorcontrib>Schweiger, Hans-Georg</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tymms, Martin J.</au><au>Schweiger, Hans-Georg</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tandemly Repeated Nonribosomal DNA Sequences in the Chloroplast Genome of an Acetabularia mediterranea Strain</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-03-01</date><risdate>1985</risdate><volume>82</volume><issue>6</issue><spage>1706</spage><epage>1710</epage><pages>1706-1710</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A purified chloroplast fraction was prepared from caps of the giant unicellular green alga Acetabularia mediterranea (strain 17). High molecular weight DNA obtained from these chloroplasts contains at least five copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species Acetabularia crenulata or chloroplast DNA from spinach.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2984664</pmid><doi>10.1073/pnas.82.6.1706</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (6), p.1706-1710 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acetabularia - genetics Acetabularia crenulata Biological and medical sciences Cell structures and functions Chlorophyta - genetics Chloroplast DNA Chloroplast, photosynthetic membrane and photosynthesis Chloroplasts Chloroplasts - metabolism Cloning, Molecular DNA DNA - genetics DNA Restriction Enzymes Enzymes Fundamental and applied biological sciences. Psychology Gels Genetic hybridization Genomes Molecular and cellular biology Molecular weight Repetitive Sequences, Nucleic Acid rRNA genes Sodium |
| title | Tandemly Repeated Nonribosomal DNA Sequences in the Chloroplast Genome of an Acetabularia mediterranea Strain |
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