Quantitative polymerase chain reaction coupled with sodium dodecyl sulfate and propidium monoazide for detection of viable Staphylococcus aureus in milk

Conventional quantitative PCR (qPCR) are unable to differentiate DNA of viable Staphylococcus aureus cells from dead ones. The aim of this study was to use sodium dodecyl sulfate (SDS) and propidium monoazide (PMA) coupled with lysostaphin to detect viable Staph. aureus. The cell suspensions were tr...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of dairy science 2018-03
Hauptverfasser:	Dong, Lei, Liu, Huimin, Meng, Lu, Xing, Mengru, Wang, Jiaqi, Wang, Cheng, Chen, He, Zheng, Nan
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!