Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design
We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field...
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| Veröffentlicht in: | Lab on a chip 2017-11, Vol.17 (23), p.459-469 |
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| container_end_page | 469 |
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| container_issue | 23 |
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| container_title | Lab on a chip |
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| creator | Ung, W. L Mutafopulos, K Spink, P Rambach, R. W Franke, T Weitz, D. A |
| description | We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.
We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. |
| doi_str_mv | 10.1039/c7lc00715a |
| format | Article |
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We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation.</description><subject>Actuation</subject><subject>Animals</subject><subject>Cell Separation - instrumentation</subject><subject>Cell Separation - methods</subject><subject>Design parameters</subject><subject>Dogs</subject><subject>Equipment Design</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>K562 Cells</subject><subject>Madin Darby Canine Kidney Cells</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Sound</subject><subject>Surface acoustic waves</subject><subject>Three dimensional flow</subject><subject>Wave excitation</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c1LwzAYBvAgipvTi3cl4kWEatKkbXIcdX4x8KLnkr5Ntox-zKRV9t_buTnBg6cE8uPlyfMidErJDSVM3kJSAiEJjdQeGlKesIBQIfd3d5kM0JH3C0JoxGNxiAahkJJzJofoeVLPVQ26wL5zRoHGCprOtxbwp_rQGHRZYt-41tYznK8wu8OVBdeYsrOFhQDmdokL7e2sPkYHRpVen2zPEXq7n7ymj8H05eEpHU8DYFK2gYxNkhPgAuIIVC6YYLFUjAujlYHE0EirkLKIa4hMLgrQjIS5znXEOFNxyEboajN36Zr3Tvs2q6xf51S17qNnVHIZS94309PLP3TRdK7u02UhoUTECQnX6nqj-n9577TJls5Wyq0ySrJ1w1maTNPvhsc9Pt-O7PJKFzv6U2kPLjbAedi9_q4oWxamN2f_GfYFAWuKqw</recordid><startdate>20171121</startdate><enddate>20171121</enddate><creator>Ung, W. 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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2017-11-21</date><risdate>2017</risdate><volume>17</volume><issue>23</issue><spage>459</spage><epage>469</epage><pages>459-469</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.
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| ispartof | Lab on a chip, 2017-11, Vol.17 (23), p.459-469 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Actuation Animals Cell Separation - instrumentation Cell Separation - methods Design parameters Dogs Equipment Design Fluorescence Humans K562 Cells Madin Darby Canine Kidney Cells Microfluidic Analytical Techniques - instrumentation Sound Surface acoustic waves Three dimensional flow Wave excitation |
| title | Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design |
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