Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay
Early diagnosis of pancreatic cancer (PC) is critical to reduce the mortality rate of this disease. Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme linked immunosorbent assay and...
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| Veröffentlicht in: | Nanotechnology 2017-11, Vol.28 (45), p.455101-455101 |
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| container_end_page | 455101 |
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| container_issue | 45 |
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| container_title | Nanotechnology |
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| creator | Banaei, Nariman Foley, Anne Houghton, Jean Marie Sun, Yubing Kim, Byung |
| description | Early diagnosis of pancreatic cancer (PC) is critical to reduce the mortality rate of this disease. Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme linked immunosorbent assay and radioimmunoassay are two common platforms for detection of biomarkers; however, they suffer from some limitation. This study demonstrates a novel system for multiplex detection of pancreatic biomarkers CA19-9, MMP7 and MUC4 in sera samples with high sensitivity using surface enhanced Raman spectroscopy. Measuring the levels of these biomarkers in PC patients, pancreatitis patients, and healthy individuals reveals the unique expression pattern of these markers in PC patients, suggesting the great potential of using this approach for early diagnostics of PCs. |
| doi_str_mv | 10.1088/1361-6528/aa8e8c |
| format | Article |
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Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme linked immunosorbent assay and radioimmunoassay are two common platforms for detection of biomarkers; however, they suffer from some limitation. This study demonstrates a novel system for multiplex detection of pancreatic biomarkers CA19-9, MMP7 and MUC4 in sera samples with high sensitivity using surface enhanced Raman spectroscopy. Measuring the levels of these biomarkers in PC patients, pancreatitis patients, and healthy individuals reveals the unique expression pattern of these markers in PC patients, suggesting the great potential of using this approach for early diagnostics of PCs.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/1361-6528/aa8e8c</identifier><identifier>PMID: 28937361</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Adult ; Aged, 80 and over ; Antigens, Tumor-Associated, Carbohydrate - metabolism ; biomarker detection ; Biomarkers, Tumor - metabolism ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Humans ; Immunoassay - methods ; Male ; Matrix Metalloproteinase 7 - metabolism ; Middle Aged ; Mucin-4 - metabolism ; pancreatic cancer ; Pancreatic Neoplasms - metabolism ; Raman spectroscopy ; SERS ; Spectrum Analysis, Raman - methods</subject><ispartof>Nanotechnology, 2017-11, Vol.28 (45), p.455101-455101</ispartof><rights>2017 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-d60046ba9c538cc268213f2f245b7f43880f0c8ff0a6eb7ef887f05247ffac873</citedby><cites>FETCH-LOGICAL-c370t-d60046ba9c538cc268213f2f245b7f43880f0c8ff0a6eb7ef887f05247ffac873</cites><orcidid>0000-0002-6831-3383 ; 0000-0002-9230-7514</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1361-6528/aa8e8c/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,776,780,27903,27904,53824,53871</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28937361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banaei, Nariman</creatorcontrib><creatorcontrib>Foley, Anne</creatorcontrib><creatorcontrib>Houghton, Jean Marie</creatorcontrib><creatorcontrib>Sun, Yubing</creatorcontrib><creatorcontrib>Kim, Byung</creatorcontrib><title>Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay</title><title>Nanotechnology</title><addtitle>NANO</addtitle><addtitle>Nanotechnology</addtitle><description>Early diagnosis of pancreatic cancer (PC) is critical to reduce the mortality rate of this disease. Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme linked immunosorbent assay and radioimmunoassay are two common platforms for detection of biomarkers; however, they suffer from some limitation. This study demonstrates a novel system for multiplex detection of pancreatic biomarkers CA19-9, MMP7 and MUC4 in sera samples with high sensitivity using surface enhanced Raman spectroscopy. Measuring the levels of these biomarkers in PC patients, pancreatitis patients, and healthy individuals reveals the unique expression pattern of these markers in PC patients, suggesting the great potential of using this approach for early diagnostics of PCs.</description><subject>Adult</subject><subject>Aged, 80 and over</subject><subject>Antigens, Tumor-Associated, Carbohydrate - metabolism</subject><subject>biomarker detection</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Male</subject><subject>Matrix Metalloproteinase 7 - metabolism</subject><subject>Middle Aged</subject><subject>Mucin-4 - metabolism</subject><subject>pancreatic cancer</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Raman spectroscopy</subject><subject>SERS</subject><subject>Spectrum Analysis, Raman - methods</subject><issn>0957-4484</issn><issn>1361-6528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LwzAYh4Mobk7vniRHBeuSNm3So4z5ARNh03NI00Qy26YmLbj_3pTOnRQCCeF5P34PAJcY3WHE2BwnGY6yNGZzIZhi8ghMD1_HYIrylEaEMDIBZ95vEcKYxfgUTGKWJzSAU7B-6avOtJX6hqXqlOyMbaDVsBWNdEp0RkIZnsrBwthauE_lPOy9aT6ggJvlehMVwqsSmrruGyu8F7tzcKJF5dXF_p6B94fl2-IpWr0-Pi_uV5FMKOqiMkOIZIXIZZowKeMsrJboWMckLagmCWNII8m0RiJTBVWaMapRGhOqtZCMJjNwPfZtnf3qle94bbxUVSUaZXvPcU7ijJI8G1A0otJZ753SvHUmpNlxjPhgkg_a-KCNjyZDydW-e1_UqjwU_KoLwM0IGNvyre1dE8LyRjQ2MJyk4aQYYd6WOrC3f7D_zv4Bap2Lag</recordid><startdate>20171110</startdate><enddate>20171110</enddate><creator>Banaei, Nariman</creator><creator>Foley, Anne</creator><creator>Houghton, Jean Marie</creator><creator>Sun, Yubing</creator><creator>Kim, Byung</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6831-3383</orcidid><orcidid>https://orcid.org/0000-0002-9230-7514</orcidid></search><sort><creationdate>20171110</creationdate><title>Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay</title><author>Banaei, Nariman ; Foley, Anne ; Houghton, Jean Marie ; Sun, Yubing ; Kim, Byung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-d60046ba9c538cc268213f2f245b7f43880f0c8ff0a6eb7ef887f05247ffac873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged, 80 and over</topic><topic>Antigens, Tumor-Associated, Carbohydrate - metabolism</topic><topic>biomarker detection</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Male</topic><topic>Matrix Metalloproteinase 7 - metabolism</topic><topic>Middle Aged</topic><topic>Mucin-4 - metabolism</topic><topic>pancreatic cancer</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Raman spectroscopy</topic><topic>SERS</topic><topic>Spectrum Analysis, Raman - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banaei, Nariman</creatorcontrib><creatorcontrib>Foley, Anne</creatorcontrib><creatorcontrib>Houghton, Jean Marie</creatorcontrib><creatorcontrib>Sun, Yubing</creatorcontrib><creatorcontrib>Kim, Byung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banaei, Nariman</au><au>Foley, Anne</au><au>Houghton, Jean Marie</au><au>Sun, Yubing</au><au>Kim, Byung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay</atitle><jtitle>Nanotechnology</jtitle><stitle>NANO</stitle><addtitle>Nanotechnology</addtitle><date>2017-11-10</date><risdate>2017</risdate><volume>28</volume><issue>45</issue><spage>455101</spage><epage>455101</epage><pages>455101-455101</pages><issn>0957-4484</issn><eissn>1361-6528</eissn><coden>NNOTER</coden><abstract>Early diagnosis of pancreatic cancer (PC) is critical to reduce the mortality rate of this disease. Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme linked immunosorbent assay and radioimmunoassay are two common platforms for detection of biomarkers; however, they suffer from some limitation. This study demonstrates a novel system for multiplex detection of pancreatic biomarkers CA19-9, MMP7 and MUC4 in sera samples with high sensitivity using surface enhanced Raman spectroscopy. 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| ispartof | Nanotechnology, 2017-11, Vol.28 (45), p.455101-455101 |
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| source | MEDLINE; IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | Adult Aged, 80 and over Antigens, Tumor-Associated, Carbohydrate - metabolism biomarker detection Biomarkers, Tumor - metabolism Enzyme-Linked Immunosorbent Assay - methods Female Humans Immunoassay - methods Male Matrix Metalloproteinase 7 - metabolism Middle Aged Mucin-4 - metabolism pancreatic cancer Pancreatic Neoplasms - metabolism Raman spectroscopy SERS Spectrum Analysis, Raman - methods |
| title | Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay |
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