CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15
Proinflammatory, cytotoxic CD4 CD28 T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4 CD28 T cells were stimulated with HLA-mismatched antigen presenting cells...
Gespeichert in:
| Veröffentlicht in: | American journal of transplantation 2018-02, Vol.18 (2), p.341 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | 341 |
| container_title | American journal of transplantation |
| container_volume | 18 |
| creator | Dedeoglu, B Litjens, N H R Klepper, M Kraaijeveld, R Verschoor, W Baan, C C Betjes, M G H |
| description | Proinflammatory, cytotoxic CD4
CD28
T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4
CD28
T cells were stimulated with HLA-mismatched antigen presenting cells in the absence/presence of exogenous cytokines. Alloreactive potential was evaluated based on proliferation, degranulation, cytotoxicity, and cytokine production. Further, their suppressive capacity was assessed by measuring inhibition of proliferating alloreactive CD28
T cells. CD4
CD28
T cells contained alloreactive (CD137
) T cells but did not proliferate in response to allogeneic stimulation, unless interleukin (IL)-15 was added. However, they could proliferate on stimulation with cytomegalovirus antigen without exogenous cytokines. IL-15 increased the frequency of proliferating alloreactive CD4
CD28
T cells to 30.5% without inducing CD28 expression (P |
| doi_str_mv | 10.1111/ajt.14480 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_28858434</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>28858434</sourcerecordid><originalsourceid>FETCH-pubmed_primary_288584343</originalsourceid><addsrcrecordid>eNqFjrsKwjAUQIMg1tfgD8jdpTVpo2aviqND9xLtFVJvY8lD8O910NmznOUMh7GF4Jn4sNZtyISUig_YWGw5T7dCFgmbeN9yLna5ykcsyZXaKFnIMTuXewkrKPe5AhuJoIIrEnnQDsE-Amiih0N9DeaJEC2h9-CD6SLpgA1cXmBsQEcY78amYjNjw5smj_Ovp2x5PFTlKe3jpcOm7p3ptHvVv4Xib_AG3Fc-gw</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Dedeoglu, B ; Litjens, N H R ; Klepper, M ; Kraaijeveld, R ; Verschoor, W ; Baan, C C ; Betjes, M G H</creator><creatorcontrib>Dedeoglu, B ; Litjens, N H R ; Klepper, M ; Kraaijeveld, R ; Verschoor, W ; Baan, C C ; Betjes, M G H</creatorcontrib><description>Proinflammatory, cytotoxic CD4
CD28
T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4
CD28
T cells were stimulated with HLA-mismatched antigen presenting cells in the absence/presence of exogenous cytokines. Alloreactive potential was evaluated based on proliferation, degranulation, cytotoxicity, and cytokine production. Further, their suppressive capacity was assessed by measuring inhibition of proliferating alloreactive CD28
T cells. CD4
CD28
T cells contained alloreactive (CD137
) T cells but did not proliferate in response to allogeneic stimulation, unless interleukin (IL)-15 was added. However, they could proliferate on stimulation with cytomegalovirus antigen without exogenous cytokines. IL-15 increased the frequency of proliferating alloreactive CD4
CD28
T cells to 30.5% without inducing CD28 expression (P < .05). After allogeneic stimulation together with IL-15 and IL-21, frequency of degranulating CD107a
CD4
CD28
T cells increased significantly from 0.6% to 5.8% (P < .001). Granzyme B and perforin positivity remained similar, but production of interferon-γ and tumor necrosis factor-α increased by the combination of IL-15 and IL-21 (P < .001 and P < .05, respectively). Finally, CD4
CD28
T cells did not show significant suppression. Thus, CD4
CD28
T cells represent a population with absent alloreactivity unless IL-15 is present.</description><identifier>EISSN: 1600-6143</identifier><identifier>DOI: 10.1111/ajt.14480</identifier><identifier>PMID: 28858434</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Antigen-Presenting Cells - immunology ; CD28 Antigens - metabolism ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - metabolism ; Female ; Follow-Up Studies ; Humans ; Interleukin-15 - metabolism ; Interleukins - metabolism ; Kidney Failure, Chronic - surgery ; Kidney Transplantation ; Lymphocyte Activation - immunology ; Male ; Middle Aged ; Prognosis ; T-Lymphocytes, Cytotoxic - immunology</subject><ispartof>American journal of transplantation, 2018-02, Vol.18 (2), p.341</ispartof><rights>2017 The American Society of Transplantation and the American Society of Transplant Surgeons.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28858434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dedeoglu, B</creatorcontrib><creatorcontrib>Litjens, N H R</creatorcontrib><creatorcontrib>Klepper, M</creatorcontrib><creatorcontrib>Kraaijeveld, R</creatorcontrib><creatorcontrib>Verschoor, W</creatorcontrib><creatorcontrib>Baan, C C</creatorcontrib><creatorcontrib>Betjes, M G H</creatorcontrib><title>CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15</title><title>American journal of transplantation</title><addtitle>Am J Transplant</addtitle><description>Proinflammatory, cytotoxic CD4
CD28
T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4
CD28
T cells were stimulated with HLA-mismatched antigen presenting cells in the absence/presence of exogenous cytokines. Alloreactive potential was evaluated based on proliferation, degranulation, cytotoxicity, and cytokine production. Further, their suppressive capacity was assessed by measuring inhibition of proliferating alloreactive CD28
T cells. CD4
CD28
T cells contained alloreactive (CD137
) T cells but did not proliferate in response to allogeneic stimulation, unless interleukin (IL)-15 was added. However, they could proliferate on stimulation with cytomegalovirus antigen without exogenous cytokines. IL-15 increased the frequency of proliferating alloreactive CD4
CD28
T cells to 30.5% without inducing CD28 expression (P < .05). After allogeneic stimulation together with IL-15 and IL-21, frequency of degranulating CD107a
CD4
CD28
T cells increased significantly from 0.6% to 5.8% (P < .001). Granzyme B and perforin positivity remained similar, but production of interferon-γ and tumor necrosis factor-α increased by the combination of IL-15 and IL-21 (P < .001 and P < .05, respectively). Finally, CD4
CD28
T cells did not show significant suppression. Thus, CD4
CD28
T cells represent a population with absent alloreactivity unless IL-15 is present.</description><subject>Adult</subject><subject>Aged</subject><subject>Antigen-Presenting Cells - immunology</subject><subject>CD28 Antigens - metabolism</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Interleukin-15 - metabolism</subject><subject>Interleukins - metabolism</subject><subject>Kidney Failure, Chronic - surgery</subject><subject>Kidney Transplantation</subject><subject>Lymphocyte Activation - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Prognosis</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>1600-6143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjrsKwjAUQIMg1tfgD8jdpTVpo2aviqND9xLtFVJvY8lD8O910NmznOUMh7GF4Jn4sNZtyISUig_YWGw5T7dCFgmbeN9yLna5ykcsyZXaKFnIMTuXewkrKPe5AhuJoIIrEnnQDsE-Amiih0N9DeaJEC2h9-CD6SLpgA1cXmBsQEcY78amYjNjw5smj_Ovp2x5PFTlKe3jpcOm7p3ptHvVv4Xib_AG3Fc-gw</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Dedeoglu, B</creator><creator>Litjens, N H R</creator><creator>Klepper, M</creator><creator>Kraaijeveld, R</creator><creator>Verschoor, W</creator><creator>Baan, C C</creator><creator>Betjes, M G H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201802</creationdate><title>CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15</title><author>Dedeoglu, B ; Litjens, N H R ; Klepper, M ; Kraaijeveld, R ; Verschoor, W ; Baan, C C ; Betjes, M G H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_288584343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigen-Presenting Cells - immunology</topic><topic>CD28 Antigens - metabolism</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Interleukin-15 - metabolism</topic><topic>Interleukins - metabolism</topic><topic>Kidney Failure, Chronic - surgery</topic><topic>Kidney Transplantation</topic><topic>Lymphocyte Activation - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Prognosis</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dedeoglu, B</creatorcontrib><creatorcontrib>Litjens, N H R</creatorcontrib><creatorcontrib>Klepper, M</creatorcontrib><creatorcontrib>Kraaijeveld, R</creatorcontrib><creatorcontrib>Verschoor, W</creatorcontrib><creatorcontrib>Baan, C C</creatorcontrib><creatorcontrib>Betjes, M G H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>American journal of transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dedeoglu, B</au><au>Litjens, N H R</au><au>Klepper, M</au><au>Kraaijeveld, R</au><au>Verschoor, W</au><au>Baan, C C</au><au>Betjes, M G H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15</atitle><jtitle>American journal of transplantation</jtitle><addtitle>Am J Transplant</addtitle><date>2018-02</date><risdate>2018</risdate><volume>18</volume><issue>2</issue><spage>341</spage><pages>341-</pages><eissn>1600-6143</eissn><abstract>Proinflammatory, cytotoxic CD4
CD28
T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4
CD28
T cells were stimulated with HLA-mismatched antigen presenting cells in the absence/presence of exogenous cytokines. Alloreactive potential was evaluated based on proliferation, degranulation, cytotoxicity, and cytokine production. Further, their suppressive capacity was assessed by measuring inhibition of proliferating alloreactive CD28
T cells. CD4
CD28
T cells contained alloreactive (CD137
) T cells but did not proliferate in response to allogeneic stimulation, unless interleukin (IL)-15 was added. However, they could proliferate on stimulation with cytomegalovirus antigen without exogenous cytokines. IL-15 increased the frequency of proliferating alloreactive CD4
CD28
T cells to 30.5% without inducing CD28 expression (P < .05). After allogeneic stimulation together with IL-15 and IL-21, frequency of degranulating CD107a
CD4
CD28
T cells increased significantly from 0.6% to 5.8% (P < .001). Granzyme B and perforin positivity remained similar, but production of interferon-γ and tumor necrosis factor-α increased by the combination of IL-15 and IL-21 (P < .001 and P < .05, respectively). Finally, CD4
CD28
T cells did not show significant suppression. Thus, CD4
CD28
T cells represent a population with absent alloreactivity unless IL-15 is present.</abstract><cop>United States</cop><pmid>28858434</pmid><doi>10.1111/ajt.14480</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1600-6143 |
| ispartof | American journal of transplantation, 2018-02, Vol.18 (2), p.341 |
| issn | 1600-6143 |
| language | eng |
| recordid | cdi_pubmed_primary_28858434 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Aged Antigen-Presenting Cells - immunology CD28 Antigens - metabolism CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism Female Follow-Up Studies Humans Interleukin-15 - metabolism Interleukins - metabolism Kidney Failure, Chronic - surgery Kidney Transplantation Lymphocyte Activation - immunology Male Middle Aged Prognosis T-Lymphocytes, Cytotoxic - immunology |
| title | CD4 + CD28 null T cells are not alloreactive unless stimulated by interleukin-15 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T21%3A20%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CD4%20+%20CD28%20null%20T%20cells%20are%20not%20alloreactive%20unless%20stimulated%20by%20interleukin-15&rft.jtitle=American%20journal%20of%20transplantation&rft.au=Dedeoglu,%20B&rft.date=2018-02&rft.volume=18&rft.issue=2&rft.spage=341&rft.pages=341-&rft.eissn=1600-6143&rft_id=info:doi/10.1111/ajt.14480&rft_dat=%3Cpubmed%3E28858434%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/28858434&rfr_iscdi=true |