Protection and Reactivation of Oxidative Phosphorylation in Mitochondria by a Stable Free-Radical Prostaglandin Polymer (PGBx)
A stable free-radical polymeric derivative of prostaglandin B1(PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBxis characterized by a singleline elect...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-04, Vol.76 (4), p.1598-1602 |
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| container_issue | 4 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 76 |
| creator | Polis, B. David Polis, Edith Kwong, Sara |
| description | A stable free-radical polymeric derivative of prostaglandin B1(PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBxis characterized by a singleline electron spin resonance spectrum that is stable at room temperature. PGBxrestores phosphorylating ability and net ATP synthesis in isolated mitochondria aged for 4 days at 0 degrees C and protects against further degradation of phosphorylating activity when such aged mitochondria are preincubated at 28 degrees C in the absence of adenine nucleotide phosphate acceptors. This compound has been reported to exert beneficial effects in vivo in experimental pathological conditions, such as regional ischemia, in which the mitochondria of the ischemic region may have been damaged. |
| doi_str_mv | 10.1073/pnas.76.4.1598 |
| format | Article |
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David ; Polis, Edith ; Kwong, Sara</creator><creatorcontrib>Polis, B. David ; Polis, Edith ; Kwong, Sara</creatorcontrib><description>A stable free-radical polymeric derivative of prostaglandin B1(PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBxis characterized by a singleline electron spin resonance spectrum that is stable at room temperature. PGBxrestores phosphorylating ability and net ATP synthesis in isolated mitochondria aged for 4 days at 0 degrees C and protects against further degradation of phosphorylating activity when such aged mitochondria are preincubated at 28 degrees C in the absence of adenine nucleotide phosphate acceptors. This compound has been reported to exert beneficial effects in vivo in experimental pathological conditions, such as regional ischemia, in which the mitochondria of the ischemic region may have been damaged.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.76.4.1598</identifier><identifier>PMID: 287000</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adenosine Triphosphate - biosynthesis ; Animals ; Biochemistry ; City states ; Electron paramagnetic resonance ; Free Radicals ; Kinetics ; Mitochondria ; Mitochondria, Liver - drug effects ; Mitochondria, Liver - metabolism ; Molecular weight ; Nucleotides ; Oxidative phosphorylation ; Oxidative Phosphorylation - drug effects ; Oxygen Consumption - drug effects ; Phosphates ; Phosphorylation ; Polymers - pharmacology ; Prostaglandins ; Prostaglandins - pharmacology ; Prostaglandins B - pharmacology ; Rats</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1979-04, Vol.76 (4), p.1598-1602</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-24afb7e362441f86db1b33c3f4f793415dd167df0487259ff30f1623278333853</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/76/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/69559$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/69559$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/287000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Polis, B. David</creatorcontrib><creatorcontrib>Polis, Edith</creatorcontrib><creatorcontrib>Kwong, Sara</creatorcontrib><title>Protection and Reactivation of Oxidative Phosphorylation in Mitochondria by a Stable Free-Radical Prostaglandin Polymer (PGBx)</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A stable free-radical polymeric derivative of prostaglandin B1(PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBxis characterized by a singleline electron spin resonance spectrum that is stable at room temperature. PGBxrestores phosphorylating ability and net ATP synthesis in isolated mitochondria aged for 4 days at 0 degrees C and protects against further degradation of phosphorylating activity when such aged mitochondria are preincubated at 28 degrees C in the absence of adenine nucleotide phosphate acceptors. This compound has been reported to exert beneficial effects in vivo in experimental pathological conditions, such as regional ischemia, in which the mitochondria of the ischemic region may have been damaged.</description><subject>Adenosine Triphosphate - biosynthesis</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>City states</subject><subject>Electron paramagnetic resonance</subject><subject>Free Radicals</subject><subject>Kinetics</subject><subject>Mitochondria</subject><subject>Mitochondria, Liver - drug effects</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Molecular weight</subject><subject>Nucleotides</subject><subject>Oxidative phosphorylation</subject><subject>Oxidative Phosphorylation - drug effects</subject><subject>Oxygen Consumption - drug effects</subject><subject>Phosphates</subject><subject>Phosphorylation</subject><subject>Polymers - pharmacology</subject><subject>Prostaglandins</subject><subject>Prostaglandins - pharmacology</subject><subject>Prostaglandins B - pharmacology</subject><subject>Rats</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1vEzEUtBBfIXDlgEDyCcFhF3-t7T1woBUtSEWNCpwt767duHLWwXai5NLfjsOWqFw4WeOZefOeBoCXGNUYCfphPepUC16zGjetfABmGLW44qxFD8EMISIqyQh7Cp6ldIMQahuJnoDHRIoCZuB2EUM2fXZhhHoc4JXRBWz1n49g4eXODQVsDVwsQ1ovQ9z7iXQj_OZy6JdhHKLTsNtDDb9n3XkDz6Ix1ZUeXK89LAkp62tfxhfPIvj9ykT4bnF-snv_HDyy2ifz4u6dg59nn3-cfqkuLs-_nn66qPqG4FwRpm0nDOWEMWwlHzrcUdpTy6xoKcPNMGAuBouYFKRpraXIYk4oEZJSKhs6Bx-nuetNtzJDb8YctVfr6FY67lXQTv3LjG6prsNWUUkZFcX_9s4fw6-NSVmtXOqNL0eZsElKME4kL2FzUE_CvlydorHHDIzUoS916EsJrpg69FUMr-9vdpRPBd0LPtj-kke7shvvs9nlInzzP2HhX038TcohHgW8bZqW_gYzdrPM</recordid><startdate>19790401</startdate><enddate>19790401</enddate><creator>Polis, B. David</creator><creator>Polis, Edith</creator><creator>Kwong, Sara</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790401</creationdate><title>Protection and Reactivation of Oxidative Phosphorylation in Mitochondria by a Stable Free-Radical Prostaglandin Polymer (PGBx)</title><author>Polis, B. David ; Polis, Edith ; Kwong, Sara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-24afb7e362441f86db1b33c3f4f793415dd167df0487259ff30f1623278333853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Adenosine Triphosphate - biosynthesis</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>City states</topic><topic>Electron paramagnetic resonance</topic><topic>Free Radicals</topic><topic>Kinetics</topic><topic>Mitochondria</topic><topic>Mitochondria, Liver - drug effects</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Molecular weight</topic><topic>Nucleotides</topic><topic>Oxidative phosphorylation</topic><topic>Oxidative Phosphorylation - drug effects</topic><topic>Oxygen Consumption - drug effects</topic><topic>Phosphates</topic><topic>Phosphorylation</topic><topic>Polymers - pharmacology</topic><topic>Prostaglandins</topic><topic>Prostaglandins - pharmacology</topic><topic>Prostaglandins B - pharmacology</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polis, B. David</creatorcontrib><creatorcontrib>Polis, Edith</creatorcontrib><creatorcontrib>Kwong, Sara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polis, B. David</au><au>Polis, Edith</au><au>Kwong, Sara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protection and Reactivation of Oxidative Phosphorylation in Mitochondria by a Stable Free-Radical Prostaglandin Polymer (PGBx)</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-04-01</date><risdate>1979</risdate><volume>76</volume><issue>4</issue><spage>1598</spage><epage>1602</epage><pages>1598-1602</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A stable free-radical polymeric derivative of prostaglandin B1(PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBxis characterized by a singleline electron spin resonance spectrum that is stable at room temperature. PGBxrestores phosphorylating ability and net ATP synthesis in isolated mitochondria aged for 4 days at 0 degrees C and protects against further degradation of phosphorylating activity when such aged mitochondria are preincubated at 28 degrees C in the absence of adenine nucleotide phosphate acceptors. This compound has been reported to exert beneficial effects in vivo in experimental pathological conditions, such as regional ischemia, in which the mitochondria of the ischemic region may have been damaged.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>287000</pmid><doi>10.1073/pnas.76.4.1598</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-04, Vol.76 (4), p.1598-1602 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenosine Triphosphate - biosynthesis Animals Biochemistry City states Electron paramagnetic resonance Free Radicals Kinetics Mitochondria Mitochondria, Liver - drug effects Mitochondria, Liver - metabolism Molecular weight Nucleotides Oxidative phosphorylation Oxidative Phosphorylation - drug effects Oxygen Consumption - drug effects Phosphates Phosphorylation Polymers - pharmacology Prostaglandins Prostaglandins - pharmacology Prostaglandins B - pharmacology Rats |
| title | Protection and Reactivation of Oxidative Phosphorylation in Mitochondria by a Stable Free-Radical Prostaglandin Polymer (PGBx) |
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