High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator
We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy...
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| Veröffentlicht in: | Journal of biomedical optics 2016-12, Vol.21 (12), p.126019-126019 |
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| container_end_page | 126019 |
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| container_issue | 12 |
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| container_title | Journal of biomedical optics |
| container_volume | 21 |
| creator | Coquoz, Séverine Nahas, Amir Sison, Miguel Lopez, Antonio Lasser, Theo |
| description | We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours. |
| doi_str_mv | 10.1117/1.JBO.21.12.126019 |
| format | Article |
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A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.21.12.126019</identifier><identifier>PMID: 28009028</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Cytological Techniques - methods ; Dictyostelium ; Equipment Design ; Escherichia coli ; HeLa Cells ; Humans ; Interferometry - instrumentation ; Interferometry - methods ; Microscopy, Video ; Optical Imaging - instrumentation ; Optical Imaging - methods</subject><ispartof>Journal of biomedical optics, 2016-12, Vol.21 (12), p.126019-126019</ispartof><rights>The Authors. 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Biomed. Opt</addtitle><description>We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.</description><subject>Cytological Techniques - methods</subject><subject>Dictyostelium</subject><subject>Equipment Design</subject><subject>Escherichia coli</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Interferometry - instrumentation</subject><subject>Interferometry - methods</subject><subject>Microscopy, Video</subject><subject>Optical Imaging - instrumentation</subject><subject>Optical Imaging - methods</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOHDEQRa2IKBDID2QR9ZJNDy73-NFLwiOAkGCRKLtYHnd5xqhf2G6i8PUxaR4LIiRLtlznXKkuIZ-BLgBAHsDi4uvVgsECWD6CQv2O7AAXtGRMwVZ-U1WVlRBqm3yM8YZSqkQtPpBtpiitKVM75NeZX2_KOCI2xbgxEcu48S75fl3YoeuGvhxN2hS3k-mTTyb5O5y5wndm_YD99nluitHj_YAt2hS8LYxNk0lD2CPvnWkjfnq8d8mP05PvR2fl5dW386PDy9IuhUwlZ2DdSjLacCmU5I6Cg2Vjaqi4odS4WtAVX1VWohCykci5Qts0vBbG0YpXu2R_zh3DcDthTLrz0WLbmh6HKWpQnEm1zFpG2YzaMMQY0Okx5F3CHw1UP_SqQedeNQMNTM-9ZunLY_606rB5Vp6KzMDBDMRchL4ZptDnfd-O_Pk_44W79-Mr69_nYUjetnh9fPp6Pjau-gsbOJ7P</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Coquoz, Séverine</creator><creator>Nahas, Amir</creator><creator>Sison, Miguel</creator><creator>Lopez, Antonio</creator><creator>Lasser, Theo</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161201</creationdate><title>High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator</title><author>Coquoz, Séverine ; Nahas, Amir ; Sison, Miguel ; Lopez, Antonio ; Lasser, Theo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-521cfb720d576875f01f14da9135a00af960b5b3c7e667d7e558ecdd596af0353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cytological Techniques - methods</topic><topic>Dictyostelium</topic><topic>Equipment Design</topic><topic>Escherichia coli</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Interferometry - instrumentation</topic><topic>Interferometry - methods</topic><topic>Microscopy, Video</topic><topic>Optical Imaging - instrumentation</topic><topic>Optical Imaging - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coquoz, Séverine</creatorcontrib><creatorcontrib>Nahas, Amir</creatorcontrib><creatorcontrib>Sison, Miguel</creatorcontrib><creatorcontrib>Lopez, Antonio</creatorcontrib><creatorcontrib>Lasser, Theo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coquoz, Séverine</au><au>Nahas, Amir</au><au>Sison, Miguel</au><au>Lopez, Antonio</au><au>Lasser, Theo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>21</volume><issue>12</issue><spage>126019</spage><epage>126019</epage><pages>126019-126019</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>28009028</pmid><doi>10.1117/1.JBO.21.12.126019</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1083-3668 |
| ispartof | Journal of biomedical optics, 2016-12, Vol.21 (12), p.126019-126019 |
| issn | 1083-3668 1560-2281 |
| language | eng |
| recordid | cdi_pubmed_primary_28009028 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Cytological Techniques - methods Dictyostelium Equipment Design Escherichia coli HeLa Cells Humans Interferometry - instrumentation Interferometry - methods Microscopy, Video Optical Imaging - instrumentation Optical Imaging - methods |
| title | High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator |
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