Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol
Gaboxadol (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol, THIP), a GABA receptor δ-subunit specific agonist, when present at low (μM) concentrations, preferentially binds and activates extrasynaptic (non-γ2, δ-subunit-containing) GABA Rs (Storustovu and Ebert, 2006; Richardson ., 2011, 2013). In th...
Gespeichert in:
| Veröffentlicht in: | Bio-protocol 2013-12, Vol.3 (23) |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 23 |
| container_start_page | |
| container_title | Bio-protocol |
| container_volume | 3 |
| creator | Ling, Lynne Caspary, Donald |
| description | Gaboxadol (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol, THIP), a GABA
receptor δ-subunit specific agonist, when present at low (μM) concentrations, preferentially binds and activates extrasynaptic (non-γ2, δ-subunit-containing) GABA
Rs (Storustovu and Ebert, 2006; Richardson
., 2011, 2013). In this prototype saturation binding experiment, a series of concentrations of [
H]gaboxadol (5, 10, 25, 50, 75, 100, 250 and 400 nM) will be used. GABA at 200 μM will be added into binding mixtures as a cold displacer for [
H]gaboxadol. Slide mailers are used and each requires 7 ml binding mixture. Pre-, post-washing and binding buffer is 50 mM Tris-Citrate (pH 7.1). The detailed procedure is outlined below. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_27747261</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>27747261</sourcerecordid><originalsourceid>FETCH-pubmed_primary_277472613</originalsourceid><addsrcrecordid>eNpjYuA0MjY21LUwNjJlQWJzMPAWF2cZGBgYGpsZGZqZsDNwGJmbm5gbmRlyMlg4lpbkFyWmZOanFyUWZGQmKxgreOi6JyblVySm5OcoBKUmpxYAVSg4ZealZOalKwQU5ZfkJ-fn8DCwpiXmFKfyQmluBjk31xBnD92C0qTc1JT4gqLM3MSiyniYVcYEFQAAcmk1jg</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Ling, Lynne ; Caspary, Donald</creator><creatorcontrib>Ling, Lynne ; Caspary, Donald</creatorcontrib><description>Gaboxadol (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol, THIP), a GABA
receptor δ-subunit specific agonist, when present at low (μM) concentrations, preferentially binds and activates extrasynaptic (non-γ2, δ-subunit-containing) GABA
Rs (Storustovu and Ebert, 2006; Richardson
., 2011, 2013). In this prototype saturation binding experiment, a series of concentrations of [
H]gaboxadol (5, 10, 25, 50, 75, 100, 250 and 400 nM) will be used. GABA at 200 μM will be added into binding mixtures as a cold displacer for [
H]gaboxadol. Slide mailers are used and each requires 7 ml binding mixture. Pre-, post-washing and binding buffer is 50 mM Tris-Citrate (pH 7.1). The detailed procedure is outlined below.</description><identifier>ISSN: 2331-8325</identifier><identifier>EISSN: 2331-8325</identifier><identifier>PMID: 27747261</identifier><language>eng</language><publisher>United States</publisher><ispartof>Bio-protocol, 2013-12, Vol.3 (23)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27747261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ling, Lynne</creatorcontrib><creatorcontrib>Caspary, Donald</creatorcontrib><title>Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol</title><title>Bio-protocol</title><addtitle>Bio Protoc</addtitle><description>Gaboxadol (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol, THIP), a GABA
receptor δ-subunit specific agonist, when present at low (μM) concentrations, preferentially binds and activates extrasynaptic (non-γ2, δ-subunit-containing) GABA
Rs (Storustovu and Ebert, 2006; Richardson
., 2011, 2013). In this prototype saturation binding experiment, a series of concentrations of [
H]gaboxadol (5, 10, 25, 50, 75, 100, 250 and 400 nM) will be used. GABA at 200 μM will be added into binding mixtures as a cold displacer for [
H]gaboxadol. Slide mailers are used and each requires 7 ml binding mixture. Pre-, post-washing and binding buffer is 50 mM Tris-Citrate (pH 7.1). The detailed procedure is outlined below.</description><issn>2331-8325</issn><issn>2331-8325</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpjYuA0MjY21LUwNjJlQWJzMPAWF2cZGBgYGpsZGZqZsDNwGJmbm5gbmRlyMlg4lpbkFyWmZOanFyUWZGQmKxgreOi6JyblVySm5OcoBKUmpxYAVSg4ZealZOalKwQU5ZfkJ-fn8DCwpiXmFKfyQmluBjk31xBnD92C0qTc1JT4gqLM3MSiyniYVcYEFQAAcmk1jg</recordid><startdate>20131205</startdate><enddate>20131205</enddate><creator>Ling, Lynne</creator><creator>Caspary, Donald</creator><scope>NPM</scope></search><sort><creationdate>20131205</creationdate><title>Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol</title><author>Ling, Lynne ; Caspary, Donald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_277472613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ling, Lynne</creatorcontrib><creatorcontrib>Caspary, Donald</creatorcontrib><collection>PubMed</collection><jtitle>Bio-protocol</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ling, Lynne</au><au>Caspary, Donald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol</atitle><jtitle>Bio-protocol</jtitle><addtitle>Bio Protoc</addtitle><date>2013-12-05</date><risdate>2013</risdate><volume>3</volume><issue>23</issue><issn>2331-8325</issn><eissn>2331-8325</eissn><abstract>Gaboxadol (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol, THIP), a GABA
receptor δ-subunit specific agonist, when present at low (μM) concentrations, preferentially binds and activates extrasynaptic (non-γ2, δ-subunit-containing) GABA
Rs (Storustovu and Ebert, 2006; Richardson
., 2011, 2013). In this prototype saturation binding experiment, a series of concentrations of [
H]gaboxadol (5, 10, 25, 50, 75, 100, 250 and 400 nM) will be used. GABA at 200 μM will be added into binding mixtures as a cold displacer for [
H]gaboxadol. Slide mailers are used and each requires 7 ml binding mixture. Pre-, post-washing and binding buffer is 50 mM Tris-Citrate (pH 7.1). The detailed procedure is outlined below.</abstract><cop>United States</cop><pmid>27747261</pmid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2331-8325 |
| ispartof | Bio-protocol, 2013-12, Vol.3 (23) |
| issn | 2331-8325 2331-8325 |
| language | eng |
| recordid | cdi_pubmed_primary_27747261 |
| source | EZB-FREE-00999 freely available EZB journals |
| title | Autoradiographic 3 H-Gaboxadol Receptor Binding Protocol |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T19%3A35%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autoradiographic%203%20H-Gaboxadol%20Receptor%20Binding%20Protocol&rft.jtitle=Bio-protocol&rft.au=Ling,%20Lynne&rft.date=2013-12-05&rft.volume=3&rft.issue=23&rft.issn=2331-8325&rft.eissn=2331-8325&rft_id=info:doi/&rft_dat=%3Cpubmed%3E27747261%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/27747261&rfr_iscdi=true |