Calpain mediated pulmonary vascular remodeling in hypoxia induced pulmonary hypertension
To explore the role of calpain in pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension and the underlying mechanisms. Sprague-Dawley rats were randomly divided into the hypoxia group and the normoxia control group. Right ventricular systolic pressure (RVSP) and mean pulmonary art...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2016-09, Vol.41 (9), p.929 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
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| creator | Zhang, Weifang Zhu, Tiantian Xiong, Aizhen Ge, Xiaoyue Xu, Ruilai Lu, Shegui Hu, Changping |
| description | To explore the role of calpain in pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension and the underlying mechanisms.
Sprague-Dawley rats were randomly divided into the hypoxia group and the normoxia control group. Right ventricular systolic pressure (RVSP) and mean pulmonary artery pressure (mPAP) were monitored by a method with right external jugular vein cannula. Right ventricular hypertrophy index was presented as the ratio of right ventricular weight to left ventricular weight (left ventricle plus septum weight). Levels of calpain-1, -2 and -4 mRNA in pulmonary artery were determined by real-time PCR. Levels of calpain-1, -2 and -4 protein were determined by Western blot. Primary rat pulmonary arterial smooth muscle cells (PASMCs) were divided into 4 groups: a normoxia control group, a normoxia+MDL28170 group, a hypoxia group and a hypoxia+MDL28170 group. Cell proliferation was detected by MTS and flow cytometry. Levels of Ki-67 and proliferating cell nuclear antigen (PCNA) mRNA were |
| doi_str_mv | 10.11817/j.issn.1672-7347.2016.09.007 |
| format | Article |
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Sprague-Dawley rats were randomly divided into the hypoxia group and the normoxia control group. Right ventricular systolic pressure (RVSP) and mean pulmonary artery pressure (mPAP) were monitored by a method with right external jugular vein cannula. Right ventricular hypertrophy index was presented as the ratio of right ventricular weight to left ventricular weight (left ventricle plus septum weight). Levels of calpain-1, -2 and -4 mRNA in pulmonary artery were determined by real-time PCR. Levels of calpain-1, -2 and -4 protein were determined by Western blot. Primary rat pulmonary arterial smooth muscle cells (PASMCs) were divided into 4 groups: a normoxia control group, a normoxia+MDL28170 group, a hypoxia group and a hypoxia+MDL28170 group. Cell proliferation was detected by MTS and flow cytometry. Levels of Ki-67 and proliferating cell nuclear antigen (PCNA) mRNA were</description><identifier>ISSN: 1672-7347</identifier><identifier>DOI: 10.11817/j.issn.1672-7347.2016.09.007</identifier><identifier>PMID: 27640791</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Calpain - genetics ; Calpain - physiology ; Cell Proliferation ; Dipeptides - physiology ; Hypertension, Pulmonary - chemically induced ; Hypertension, Pulmonary - genetics ; Hypertension, Pulmonary - physiopathology ; Hypertrophy, Right Ventricular ; Hypoxia ; Ki-67 Antigen - drug effects ; Myocytes, Smooth Muscle - physiology ; Proliferating Cell Nuclear Antigen - drug effects ; Pulmonary Artery ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; Up-Regulation ; Vascular Remodeling - genetics ; Vascular Remodeling - physiology</subject><ispartof>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2016-09, Vol.41 (9), p.929</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27640791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Weifang</creatorcontrib><creatorcontrib>Zhu, Tiantian</creatorcontrib><creatorcontrib>Xiong, Aizhen</creatorcontrib><creatorcontrib>Ge, Xiaoyue</creatorcontrib><creatorcontrib>Xu, Ruilai</creatorcontrib><creatorcontrib>Lu, Shegui</creatorcontrib><creatorcontrib>Hu, Changping</creatorcontrib><title>Calpain mediated pulmonary vascular remodeling in hypoxia induced pulmonary hypertension</title><title>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</title><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><description>To explore the role of calpain in pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension and the underlying mechanisms.
Sprague-Dawley rats were randomly divided into the hypoxia group and the normoxia control group. Right ventricular systolic pressure (RVSP) and mean pulmonary artery pressure (mPAP) were monitored by a method with right external jugular vein cannula. Right ventricular hypertrophy index was presented as the ratio of right ventricular weight to left ventricular weight (left ventricle plus septum weight). Levels of calpain-1, -2 and -4 mRNA in pulmonary artery were determined by real-time PCR. Levels of calpain-1, -2 and -4 protein were determined by Western blot. Primary rat pulmonary arterial smooth muscle cells (PASMCs) were divided into 4 groups: a normoxia control group, a normoxia+MDL28170 group, a hypoxia group and a hypoxia+MDL28170 group. Cell proliferation was detected by MTS and flow cytometry. Levels of Ki-67 and proliferating cell nuclear antigen (PCNA) mRNA were</description><subject>Animals</subject><subject>Calpain - genetics</subject><subject>Calpain - physiology</subject><subject>Cell Proliferation</subject><subject>Dipeptides - physiology</subject><subject>Hypertension, Pulmonary - chemically induced</subject><subject>Hypertension, Pulmonary - genetics</subject><subject>Hypertension, Pulmonary - physiopathology</subject><subject>Hypertrophy, Right Ventricular</subject><subject>Hypoxia</subject><subject>Ki-67 Antigen - drug effects</subject><subject>Myocytes, Smooth Muscle - physiology</subject><subject>Proliferating Cell Nuclear Antigen - drug effects</subject><subject>Pulmonary Artery</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Up-Regulation</subject><subject>Vascular Remodeling - genetics</subject><subject>Vascular Remodeling - physiology</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVj0tLxDAUhbNQnGGcvyDduGzMa3LbpRRfMOBGwd1w06QaadPQtOL8ewM-wNW93PPdwzmEXHJGOa84XL1Tn1KgXIMoQSqggnFNWU0ZgxOy_ruvyDYlbxir61rqCs7ISoBWDGq-Ji8N9hF9KAZnPc7OFnHphzHgdCw-MLVLj1MxuWG0rvfhtcjk2zGOnx7zapf230NW3DS7kPwYzslph31y25-5Ic-3N0_Nfbl_vHtorvdl5ELPpep0q5Vh0Bln1A4rqQQ3DlsDO1AZsUI6JrEDEJApLgQzaCVqayznKDfk4ts3LiZ3OMTJDznL4bei_AL_jlg5</recordid><startdate>20160928</startdate><enddate>20160928</enddate><creator>Zhang, Weifang</creator><creator>Zhu, Tiantian</creator><creator>Xiong, Aizhen</creator><creator>Ge, Xiaoyue</creator><creator>Xu, Ruilai</creator><creator>Lu, Shegui</creator><creator>Hu, Changping</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20160928</creationdate><title>Calpain mediated pulmonary vascular remodeling in hypoxia induced pulmonary hypertension</title><author>Zhang, Weifang ; Zhu, Tiantian ; Xiong, Aizhen ; Ge, Xiaoyue ; Xu, Ruilai ; Lu, Shegui ; Hu, Changping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-4f6c64b07fbeb45a83421beacb7574126d23e03af772707f1220bad3a6dbd11a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Calpain - genetics</topic><topic>Calpain - physiology</topic><topic>Cell Proliferation</topic><topic>Dipeptides - physiology</topic><topic>Hypertension, Pulmonary - chemically induced</topic><topic>Hypertension, Pulmonary - genetics</topic><topic>Hypertension, Pulmonary - physiopathology</topic><topic>Hypertrophy, Right Ventricular</topic><topic>Hypoxia</topic><topic>Ki-67 Antigen - drug effects</topic><topic>Myocytes, Smooth Muscle - physiology</topic><topic>Proliferating Cell Nuclear Antigen - drug effects</topic><topic>Pulmonary Artery</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Up-Regulation</topic><topic>Vascular Remodeling - genetics</topic><topic>Vascular Remodeling - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Weifang</creatorcontrib><creatorcontrib>Zhu, Tiantian</creatorcontrib><creatorcontrib>Xiong, Aizhen</creatorcontrib><creatorcontrib>Ge, Xiaoyue</creatorcontrib><creatorcontrib>Xu, Ruilai</creatorcontrib><creatorcontrib>Lu, Shegui</creatorcontrib><creatorcontrib>Hu, Changping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Weifang</au><au>Zhu, Tiantian</au><au>Xiong, Aizhen</au><au>Ge, Xiaoyue</au><au>Xu, Ruilai</au><au>Lu, Shegui</au><au>Hu, Changping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calpain mediated pulmonary vascular remodeling in hypoxia induced pulmonary hypertension</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2016-09-28</date><risdate>2016</risdate><volume>41</volume><issue>9</issue><spage>929</spage><pages>929-</pages><issn>1672-7347</issn><abstract>To explore the role of calpain in pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension and the underlying mechanisms.
Sprague-Dawley rats were randomly divided into the hypoxia group and the normoxia control group. Right ventricular systolic pressure (RVSP) and mean pulmonary artery pressure (mPAP) were monitored by a method with right external jugular vein cannula. Right ventricular hypertrophy index was presented as the ratio of right ventricular weight to left ventricular weight (left ventricle plus septum weight). Levels of calpain-1, -2 and -4 mRNA in pulmonary artery were determined by real-time PCR. Levels of calpain-1, -2 and -4 protein were determined by Western blot. Primary rat pulmonary arterial smooth muscle cells (PASMCs) were divided into 4 groups: a normoxia control group, a normoxia+MDL28170 group, a hypoxia group and a hypoxia+MDL28170 group. Cell proliferation was detected by MTS and flow cytometry. Levels of Ki-67 and proliferating cell nuclear antigen (PCNA) mRNA were</abstract><cop>China</cop><pmid>27640791</pmid><doi>10.11817/j.issn.1672-7347.2016.09.007</doi></addata></record> |
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2016-09, Vol.41 (9), p.929 |
| issn | 1672-7347 |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Calpain - genetics Calpain - physiology Cell Proliferation Dipeptides - physiology Hypertension, Pulmonary - chemically induced Hypertension, Pulmonary - genetics Hypertension, Pulmonary - physiopathology Hypertrophy, Right Ventricular Hypoxia Ki-67 Antigen - drug effects Myocytes, Smooth Muscle - physiology Proliferating Cell Nuclear Antigen - drug effects Pulmonary Artery Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Up-Regulation Vascular Remodeling - genetics Vascular Remodeling - physiology |
| title | Calpain mediated pulmonary vascular remodeling in hypoxia induced pulmonary hypertension |
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