Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy
Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by ad...
Gespeichert in:
| Veröffentlicht in: | International archives of allergy and immunology 2016-01, Vol.170 (3), p.187-193 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 193 |
|---|---|
| container_issue | 3 |
| container_start_page | 187 |
| container_title | International archives of allergy and immunology |
| container_volume | 170 |
| creator | Koppes, Sjors A. Brans, Richard Ljubojevic Hadzavdic, Suzana Frings-Dresen, Monique H.W. Rustemeyer, Thomas Kezic, Sanja |
| description | Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy. |
| doi_str_mv | 10.1159/000448400 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmed_primary_27584583</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A637221286</galeid><sourcerecordid>A637221286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-5148f11b02f47d1f87aa07287b39166760b85e9aa2cdeebfd29b673ed18f7ff73</originalsourceid><addsrcrecordid>eNpdkdFrFDEQxhex2Fp98F0kIIg-rCbZZJP1QThOrYUWBa_PIbs7uUvdTbZJVrz_vjnuPKxP3zDfb4YZvqJ4QfB7QnjzAWPMmGQYPyrOCKNViXEjHucaE1lSVsnT4mmMtxhnWNZPilMquGRcVmfFn58p6DSPaOmDg6wrPQHKTTtN1q0_omvvbPIh18gbdOnMoMdR584WXUNvd1VE1qFF8pPt0GcI2bXJRvQjK7gU0U3cTa92vh7QagNBT9tnxYnRQ4TnBz0vbr5-WS2_lVffLy6Xi6uy45SkkhMmDSEtpoaJnhgptMaCStFWDalrUeNWcmi0pl0P0JqeNm0tKuiJNMIYUZ0Xn_Z7p7kdoe_yRUEPagp21GGrvLbqoePsRq39b8VpU0vJ84K3hwXB380Qkxpt7GAYtAM_R0UkZZwJIqqMvv4PvfVzcPk9RZoMcNHIJlNv9tRaD6A2oIe0iX6Yk_UuqkVdCUoJlXUG3-3BLvgYA5jj1QSrXfDqGHxmX_375pH8m3QGXu6BXzqsIRyBw_w9n9ayqw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1917357989</pqid></control><display><type>article</type><title>Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy</title><source>MEDLINE</source><source>Karger Journals</source><source>Alma/SFX Local Collection</source><creator>Koppes, Sjors A. ; Brans, Richard ; Ljubojevic Hadzavdic, Suzana ; Frings-Dresen, Monique H.W. ; Rustemeyer, Thomas ; Kezic, Sanja</creator><creatorcontrib>Koppes, Sjors A. ; Brans, Richard ; Ljubojevic Hadzavdic, Suzana ; Frings-Dresen, Monique H.W. ; Rustemeyer, Thomas ; Kezic, Sanja</creatorcontrib><description>Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy.</description><identifier>ISSN: 1018-2438</identifier><identifier>EISSN: 1423-0097</identifier><identifier>DOI: 10.1159/000448400</identifier><identifier>PMID: 27584583</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Acute phase proteins ; Administration, Topical ; Adult ; Amyloid ; Atopic dermatitis ; C-reactive protein ; Care and treatment ; Case-Control Studies ; CCL17 protein ; CCL22 protein ; Cell activation ; Ceramide ; Cytokines ; Cytokines - metabolism ; Dermatitis ; Dermatitis, Atopic - diagnosis ; Dermatitis, Atopic - metabolism ; Dermatitis, Atopic - therapy ; Dermatologic agents ; Dermatology ; Diagnosis ; Eczema ; Emollients - administration & dosage ; Epidermis ; Epidermis - metabolism ; Female ; Formulae, receipts, prescriptions ; Health aspects ; Humans ; Immunomodulation ; Inflammation ; Inflammation Mediators - metabolism ; Interleukin 10 ; Interleukin 13 ; Interleukin 2 ; Interleukin 8 ; Lymphocytes T ; Macrophages ; Magnesium ; Male ; Methods ; Middle Aged ; Molecules ; Monocyte chemoattractant protein 1 ; Original Paper ; Proteins ; Sampling ; Skin diseases ; Skin tests ; Stratum corneum ; Surgical Tape ; Thymus ; Transdermal medication ; Young Adult</subject><ispartof>International archives of allergy and immunology, 2016-01, Vol.170 (3), p.187-193</ispartof><rights>2016 The Author(s) Published by S. Karger AG, Basel</rights><rights>2016 S. Karger AG, Basel.</rights><rights>COPYRIGHT 2016 S. Karger AG</rights><rights>Copyright S. Karger AG Sep 2016</rights><rights>Copyright © 2016 by S. Karger AG, Basel 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-5148f11b02f47d1f87aa07287b39166760b85e9aa2cdeebfd29b673ed18f7ff73</citedby><cites>FETCH-LOGICAL-c521t-5148f11b02f47d1f87aa07287b39166760b85e9aa2cdeebfd29b673ed18f7ff73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,2430,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27584583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koppes, Sjors A.</creatorcontrib><creatorcontrib>Brans, Richard</creatorcontrib><creatorcontrib>Ljubojevic Hadzavdic, Suzana</creatorcontrib><creatorcontrib>Frings-Dresen, Monique H.W.</creatorcontrib><creatorcontrib>Rustemeyer, Thomas</creatorcontrib><creatorcontrib>Kezic, Sanja</creatorcontrib><title>Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy</title><title>International archives of allergy and immunology</title><addtitle>Int Arch Allergy Immunol</addtitle><description>Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy.</description><subject>Acute phase proteins</subject><subject>Administration, Topical</subject><subject>Adult</subject><subject>Amyloid</subject><subject>Atopic dermatitis</subject><subject>C-reactive protein</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>CCL17 protein</subject><subject>CCL22 protein</subject><subject>Cell activation</subject><subject>Ceramide</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Dermatitis</subject><subject>Dermatitis, Atopic - diagnosis</subject><subject>Dermatitis, Atopic - metabolism</subject><subject>Dermatitis, Atopic - therapy</subject><subject>Dermatologic agents</subject><subject>Dermatology</subject><subject>Diagnosis</subject><subject>Eczema</subject><subject>Emollients - administration & dosage</subject><subject>Epidermis</subject><subject>Epidermis - metabolism</subject><subject>Female</subject><subject>Formulae, receipts, prescriptions</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunomodulation</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interleukin 10</subject><subject>Interleukin 13</subject><subject>Interleukin 2</subject><subject>Interleukin 8</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Magnesium</subject><subject>Male</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Molecules</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Original Paper</subject><subject>Proteins</subject><subject>Sampling</subject><subject>Skin diseases</subject><subject>Skin tests</subject><subject>Stratum corneum</subject><subject>Surgical Tape</subject><subject>Thymus</subject><subject>Transdermal medication</subject><subject>Young Adult</subject><issn>1018-2438</issn><issn>1423-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><recordid>eNpdkdFrFDEQxhex2Fp98F0kIIg-rCbZZJP1QThOrYUWBa_PIbs7uUvdTbZJVrz_vjnuPKxP3zDfb4YZvqJ4QfB7QnjzAWPMmGQYPyrOCKNViXEjHucaE1lSVsnT4mmMtxhnWNZPilMquGRcVmfFn58p6DSPaOmDg6wrPQHKTTtN1q0_omvvbPIh18gbdOnMoMdR584WXUNvd1VE1qFF8pPt0GcI2bXJRvQjK7gU0U3cTa92vh7QagNBT9tnxYnRQ4TnBz0vbr5-WS2_lVffLy6Xi6uy45SkkhMmDSEtpoaJnhgptMaCStFWDalrUeNWcmi0pl0P0JqeNm0tKuiJNMIYUZ0Xn_Z7p7kdoe_yRUEPagp21GGrvLbqoePsRq39b8VpU0vJ84K3hwXB380Qkxpt7GAYtAM_R0UkZZwJIqqMvv4PvfVzcPk9RZoMcNHIJlNv9tRaD6A2oIe0iX6Yk_UuqkVdCUoJlXUG3-3BLvgYA5jj1QSrXfDqGHxmX_375pH8m3QGXu6BXzqsIRyBw_w9n9ayqw</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Koppes, Sjors A.</creator><creator>Brans, Richard</creator><creator>Ljubojevic Hadzavdic, Suzana</creator><creator>Frings-Dresen, Monique H.W.</creator><creator>Rustemeyer, Thomas</creator><creator>Kezic, Sanja</creator><general>S. Karger AG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy</title><author>Koppes, Sjors A. ; Brans, Richard ; Ljubojevic Hadzavdic, Suzana ; Frings-Dresen, Monique H.W. ; Rustemeyer, Thomas ; Kezic, Sanja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-5148f11b02f47d1f87aa07287b39166760b85e9aa2cdeebfd29b673ed18f7ff73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acute phase proteins</topic><topic>Administration, Topical</topic><topic>Adult</topic><topic>Amyloid</topic><topic>Atopic dermatitis</topic><topic>C-reactive protein</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>CCL17 protein</topic><topic>CCL22 protein</topic><topic>Cell activation</topic><topic>Ceramide</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Dermatitis</topic><topic>Dermatitis, Atopic - diagnosis</topic><topic>Dermatitis, Atopic - metabolism</topic><topic>Dermatitis, Atopic - therapy</topic><topic>Dermatologic agents</topic><topic>Dermatology</topic><topic>Diagnosis</topic><topic>Eczema</topic><topic>Emollients - administration & dosage</topic><topic>Epidermis</topic><topic>Epidermis - metabolism</topic><topic>Female</topic><topic>Formulae, receipts, prescriptions</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunomodulation</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interleukin 10</topic><topic>Interleukin 13</topic><topic>Interleukin 2</topic><topic>Interleukin 8</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Magnesium</topic><topic>Male</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Molecules</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Original Paper</topic><topic>Proteins</topic><topic>Sampling</topic><topic>Skin diseases</topic><topic>Skin tests</topic><topic>Stratum corneum</topic><topic>Surgical Tape</topic><topic>Thymus</topic><topic>Transdermal medication</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koppes, Sjors A.</creatorcontrib><creatorcontrib>Brans, Richard</creatorcontrib><creatorcontrib>Ljubojevic Hadzavdic, Suzana</creatorcontrib><creatorcontrib>Frings-Dresen, Monique H.W.</creatorcontrib><creatorcontrib>Rustemeyer, Thomas</creatorcontrib><creatorcontrib>Kezic, Sanja</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International archives of allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koppes, Sjors A.</au><au>Brans, Richard</au><au>Ljubojevic Hadzavdic, Suzana</au><au>Frings-Dresen, Monique H.W.</au><au>Rustemeyer, Thomas</au><au>Kezic, Sanja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy</atitle><jtitle>International archives of allergy and immunology</jtitle><addtitle>Int Arch Allergy Immunol</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>170</volume><issue>3</issue><spage>187</spage><epage>193</epage><pages>187-193</pages><issn>1018-2438</issn><eissn>1423-0097</eissn><abstract>Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>27584583</pmid><doi>10.1159/000448400</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1018-2438 |
| ispartof | International archives of allergy and immunology, 2016-01, Vol.170 (3), p.187-193 |
| issn | 1018-2438 1423-0097 |
| language | eng |
| recordid | cdi_pubmed_primary_27584583 |
| source | MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Acute phase proteins Administration, Topical Adult Amyloid Atopic dermatitis C-reactive protein Care and treatment Case-Control Studies CCL17 protein CCL22 protein Cell activation Ceramide Cytokines Cytokines - metabolism Dermatitis Dermatitis, Atopic - diagnosis Dermatitis, Atopic - metabolism Dermatitis, Atopic - therapy Dermatologic agents Dermatology Diagnosis Eczema Emollients - administration & dosage Epidermis Epidermis - metabolism Female Formulae, receipts, prescriptions Health aspects Humans Immunomodulation Inflammation Inflammation Mediators - metabolism Interleukin 10 Interleukin 13 Interleukin 2 Interleukin 8 Lymphocytes T Macrophages Magnesium Male Methods Middle Aged Molecules Monocyte chemoattractant protein 1 Original Paper Proteins Sampling Skin diseases Skin tests Stratum corneum Surgical Tape Thymus Transdermal medication Young Adult |
| title | Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T13%3A03%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stratum%20Corneum%20Tape%20Stripping:%20Monitoring%20of%20Inflammatory%20Mediators%20in%20Atopic%20Dermatitis%20Patients%20Using%20Topical%20Therapy&rft.jtitle=International%20archives%20of%20allergy%20and%20immunology&rft.au=Koppes,%20Sjors%20A.&rft.date=2016-01-01&rft.volume=170&rft.issue=3&rft.spage=187&rft.epage=193&rft.pages=187-193&rft.issn=1018-2438&rft.eissn=1423-0097&rft_id=info:doi/10.1159/000448400&rft_dat=%3Cgale_pubme%3EA637221286%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1917357989&rft_id=info:pmid/27584583&rft_galeid=A637221286&rfr_iscdi=true |