Involvement of ABA- and H2O2-dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress
The roles of abscisic acid (ABA) and hydrogen peroxide (H2O2) in inducing glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) activity and the possible roles of G6PDH in regulating ascorbate-glutathione (AsA-GSH) cycle were investigated in soybean (Glycine max L.) roots under drought stress. Drou...
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| Veröffentlicht in: | Plant physiology and biochemistry 2016-10, Vol.107, p.126-136 |
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| creator | Wang, Huahua Yang, Lidan Li, Yan Hou, Junjie Huang, Junjun Liang, Weihong |
| description | The roles of abscisic acid (ABA) and hydrogen peroxide (H2O2) in inducing glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) activity and the possible roles of G6PDH in regulating ascorbate-glutathione (AsA-GSH) cycle were investigated in soybean (Glycine max L.) roots under drought stress. Drought caused a marked increase of the total and cytosolic G6PDH activities and triggered a rapid ABA and H2O2 accumulation in soybean roots. Exogenous ABA or H2O2 treatment elevated the total and cytosolic G6PDH activities, whereas suppressing ABA or H2O2 production inhibited the drought-induced increase in total and cytosolic G6PDH activities, suggesting that ABA and H2O2 are required for drought-induced increase of total G6PDH activity, namely cytosolic G6PDH activity. Furthermore, ABA induced H2O2 production by stimulating NADPH oxidase activity under drought stress. Moreover, drought significantly increased the contents of AsA and GSH and the activities of key enzymes in AsA-GSH cycle, while application of G6PDH inhibitor to seedlings significantly reduced the above effect induced by drought. Taken together, these results indicate that H2O2 acting as a downstream signaling molecule of ABA mediates drought-induced increase in cytosolic G6PDH activity, and that enhanced cytosolic G6PDH activity maintains cellular redox homeostasis by regulating AsA-GSH cycle in soybean roots.
•Drought-induced increase in total G6PDH activity results from enhanced cytosolic G6PDH.•ABA and H2O2 are required for drought-induced increase in cytosolic G6PDH activity.•H2O2 acts downstream of ABA in inducing cytosolic G6PDH activity under drought stress.•Enhanced cytosolic G6PDH activity plays an important role in regulating AsA-GSH cycle. |
| doi_str_mv | 10.1016/j.plaphy.2016.05.040 |
| format | Article |
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•Drought-induced increase in total G6PDH activity results from enhanced cytosolic G6PDH.•ABA and H2O2 are required for drought-induced increase in cytosolic G6PDH activity.•H2O2 acts downstream of ABA in inducing cytosolic G6PDH activity under drought stress.•Enhanced cytosolic G6PDH activity plays an important role in regulating AsA-GSH cycle.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2016.05.040</identifier><identifier>PMID: 27285781</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Abscisic acid ; Abscisic Acid - pharmacology ; Antioxidants - metabolism ; Ascorbate-glutathione cycle ; Ascorbic Acid - metabolism ; Catalase - metabolism ; Cytosol - drug effects ; Cytosol - enzymology ; Drought resistance ; Droughts ; Enzyme Inhibitors - pharmacology ; Glucose-6-phosphate dehydrogenase ; Glucosephosphate Dehydrogenase - metabolism ; Glutathione - metabolism ; Glycine max - drug effects ; Glycine max - enzymology ; Glycine max - physiology ; Homeostasis - drug effects ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; NADPH Oxidases - metabolism ; Oxidation-Reduction - drug effects ; Oxidative damage ; Plant Roots - drug effects ; Plant Roots - enzymology ; Stress, Physiological - drug effects</subject><ispartof>Plant physiology and biochemistry, 2016-10, Vol.107, p.126-136</ispartof><rights>2016 Elsevier Masson SAS</rights><rights>Copyright © 2016 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plaphy.2016.05.040$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27285781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Huahua</creatorcontrib><creatorcontrib>Yang, Lidan</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Hou, Junjie</creatorcontrib><creatorcontrib>Huang, Junjun</creatorcontrib><creatorcontrib>Liang, Weihong</creatorcontrib><title>Involvement of ABA- and H2O2-dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>The roles of abscisic acid (ABA) and hydrogen peroxide (H2O2) in inducing glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) activity and the possible roles of G6PDH in regulating ascorbate-glutathione (AsA-GSH) cycle were investigated in soybean (Glycine max L.) roots under drought stress. Drought caused a marked increase of the total and cytosolic G6PDH activities and triggered a rapid ABA and H2O2 accumulation in soybean roots. Exogenous ABA or H2O2 treatment elevated the total and cytosolic G6PDH activities, whereas suppressing ABA or H2O2 production inhibited the drought-induced increase in total and cytosolic G6PDH activities, suggesting that ABA and H2O2 are required for drought-induced increase of total G6PDH activity, namely cytosolic G6PDH activity. Furthermore, ABA induced H2O2 production by stimulating NADPH oxidase activity under drought stress. Moreover, drought significantly increased the contents of AsA and GSH and the activities of key enzymes in AsA-GSH cycle, while application of G6PDH inhibitor to seedlings significantly reduced the above effect induced by drought. Taken together, these results indicate that H2O2 acting as a downstream signaling molecule of ABA mediates drought-induced increase in cytosolic G6PDH activity, and that enhanced cytosolic G6PDH activity maintains cellular redox homeostasis by regulating AsA-GSH cycle in soybean roots.
•Drought-induced increase in total G6PDH activity results from enhanced cytosolic G6PDH.•ABA and H2O2 are required for drought-induced increase in cytosolic G6PDH activity.•H2O2 acts downstream of ABA in inducing cytosolic G6PDH activity under drought stress.•Enhanced cytosolic G6PDH activity plays an important role in regulating AsA-GSH cycle.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Antioxidants - metabolism</subject><subject>Ascorbate-glutathione cycle</subject><subject>Ascorbic Acid - metabolism</subject><subject>Catalase - metabolism</subject><subject>Cytosol - drug effects</subject><subject>Cytosol - enzymology</subject><subject>Drought resistance</subject><subject>Droughts</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Glucose-6-phosphate dehydrogenase</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Glutathione - metabolism</subject><subject>Glycine max - drug effects</subject><subject>Glycine max - enzymology</subject><subject>Glycine max - physiology</subject><subject>Homeostasis - drug effects</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>NADPH Oxidases - metabolism</subject><subject>Oxidation-Reduction - drug effects</subject><subject>Oxidative damage</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - enzymology</subject><subject>Stress, Physiological - drug effects</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kduKFDEQhoMo7rj6BiJ5gbQ5THeSG2Fc1F1Y2Bu9DumkejpDd9IkmcF-FN_WHkYviqKon78OH0IfGW0YZd3nU7NMdhnXhm9VQ9uG7ukrtGNKCsI7TV-jHdWKEb3n6g69K-VEKeV7Kd6iOy65aqViO_TnKV7SdIEZYsVpwIevB4Jt9PiRv3DiYYHory231lTSFBw-TmeXCpCOLGMqy2grYA_j6nM6QrQFcIh4tiHWLUI84gw-_cZjmiGVaksoV0FJaw824pxSLfi8Dcl4czgfx4pLzVDKe_RmsFOBD__yPfr1_dvPh0fy_PLj6eHwTIB3XSVy0JYNqtdKWS0U95RrqQUX_aAG4bZ7tdv33SApbWkHru25aL0aHIOWS2nFPfp0813O_QzeLDnMNq_m_482wZebALYtLgGyKS5AdOBDBleNT8Ewaq5QzMncoJgrFENbs0ERfwFqfINV</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Wang, Huahua</creator><creator>Yang, Lidan</creator><creator>Li, Yan</creator><creator>Hou, Junjie</creator><creator>Huang, Junjun</creator><creator>Liang, Weihong</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201610</creationdate><title>Involvement of ABA- and H2O2-dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress</title><author>Wang, Huahua ; Yang, Lidan ; Li, Yan ; Hou, Junjie ; Huang, Junjun ; Liang, Weihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e266t-7f9a1f8b988a9382d02979323bf8f3c4739c4b6f700506ec5b235d8fc1e5277a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Antioxidants - metabolism</topic><topic>Ascorbate-glutathione cycle</topic><topic>Ascorbic Acid - metabolism</topic><topic>Catalase - metabolism</topic><topic>Cytosol - drug effects</topic><topic>Cytosol - enzymology</topic><topic>Drought resistance</topic><topic>Droughts</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Glucose-6-phosphate dehydrogenase</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>Glutathione - metabolism</topic><topic>Glycine max - drug effects</topic><topic>Glycine max - enzymology</topic><topic>Glycine max - physiology</topic><topic>Homeostasis - drug effects</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>NADPH Oxidases - metabolism</topic><topic>Oxidation-Reduction - drug effects</topic><topic>Oxidative damage</topic><topic>Plant Roots - drug effects</topic><topic>Plant Roots - enzymology</topic><topic>Stress, Physiological - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Huahua</creatorcontrib><creatorcontrib>Yang, Lidan</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Hou, Junjie</creatorcontrib><creatorcontrib>Huang, Junjun</creatorcontrib><creatorcontrib>Liang, Weihong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Huahua</au><au>Yang, Lidan</au><au>Li, Yan</au><au>Hou, Junjie</au><au>Huang, Junjun</au><au>Liang, Weihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of ABA- and H2O2-dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2016-10</date><risdate>2016</risdate><volume>107</volume><spage>126</spage><epage>136</epage><pages>126-136</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><abstract>The roles of abscisic acid (ABA) and hydrogen peroxide (H2O2) in inducing glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) activity and the possible roles of G6PDH in regulating ascorbate-glutathione (AsA-GSH) cycle were investigated in soybean (Glycine max L.) roots under drought stress. Drought caused a marked increase of the total and cytosolic G6PDH activities and triggered a rapid ABA and H2O2 accumulation in soybean roots. Exogenous ABA or H2O2 treatment elevated the total and cytosolic G6PDH activities, whereas suppressing ABA or H2O2 production inhibited the drought-induced increase in total and cytosolic G6PDH activities, suggesting that ABA and H2O2 are required for drought-induced increase of total G6PDH activity, namely cytosolic G6PDH activity. Furthermore, ABA induced H2O2 production by stimulating NADPH oxidase activity under drought stress. Moreover, drought significantly increased the contents of AsA and GSH and the activities of key enzymes in AsA-GSH cycle, while application of G6PDH inhibitor to seedlings significantly reduced the above effect induced by drought. Taken together, these results indicate that H2O2 acting as a downstream signaling molecule of ABA mediates drought-induced increase in cytosolic G6PDH activity, and that enhanced cytosolic G6PDH activity maintains cellular redox homeostasis by regulating AsA-GSH cycle in soybean roots.
•Drought-induced increase in total G6PDH activity results from enhanced cytosolic G6PDH.•ABA and H2O2 are required for drought-induced increase in cytosolic G6PDH activity.•H2O2 acts downstream of ABA in inducing cytosolic G6PDH activity under drought stress.•Enhanced cytosolic G6PDH activity plays an important role in regulating AsA-GSH cycle.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>27285781</pmid><doi>10.1016/j.plaphy.2016.05.040</doi><tpages>11</tpages></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - pharmacology Antioxidants - metabolism Ascorbate-glutathione cycle Ascorbic Acid - metabolism Catalase - metabolism Cytosol - drug effects Cytosol - enzymology Drought resistance Droughts Enzyme Inhibitors - pharmacology Glucose-6-phosphate dehydrogenase Glucosephosphate Dehydrogenase - metabolism Glutathione - metabolism Glycine max - drug effects Glycine max - enzymology Glycine max - physiology Homeostasis - drug effects Hydrogen peroxide Hydrogen Peroxide - pharmacology NADPH Oxidases - metabolism Oxidation-Reduction - drug effects Oxidative damage Plant Roots - drug effects Plant Roots - enzymology Stress, Physiological - drug effects |
| title | Involvement of ABA- and H2O2-dependent cytosolic glucose-6-phosphate dehydrogenase in maintaining redox homeostasis in soybean roots under drought stress |
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