LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells

Synthetic single-stranded DNA oligonucleotides (ssODNs) can be used to generate subtle genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior generation of DNA double-stranded breaks. However, DNA mismatch repair (MMR) suppresses the efficiency of gene modificati...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2016-04, Vol.113 (15), p.4122-4127
Hauptverfasser:	van Ravesteyn, Thomas W., Dekker, Marleen, Fish, Alexander, Sixma, Titia K., Wolters, Astrid, Dekker, Rob J., te Riele, Hein P. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Pair Mismatch Biological Sciences Cells Deoxyribonucleic acid DNA DNA - metabolism DNA Breaks, Double-Stranded DNA Mismatch Repair DNA Repair DNA, Single-Stranded E coli Escherichia coli Escherichia coli - genetics Genes Genetics Oligonucleotides - genetics Rodents
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