Hyperexpression of a Bacillus circulans xylanase gene in Escherichia coli and characterization of the gene product

A 4.0-kilobase (kb) fragment of Bacillus circulans genomic DNA inserted into pUC19 and encoding endoxylanase activity was subjected to a series of subclonings. A 1.0-kb HindIII-HincII subfragment was found to code for xylanase activity. Maximum expression levels were observed with a subclone that co...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied and Environmental Microbiology 1989-05, Vol.55 (5), p.1192-1195
Hauptverfasser:	Yang, R.C.A, MacKenzie, C.R, Bilous, D, Narang, S.A
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE AZUCARES BACILLUS Bacillus - enzymology Bacillus - genetics Biological and medical sciences Blotting, Southern Chromatography, Gel Chromatography, Ion Exchange CLONE CLONES Cloning, Molecular DNA, Bacterial - genetics Electrophoresis, Polyacrylamide Gel ENZIMAS ENZYME ESCHERICHIA Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE Gene expression Gene Expression Regulation gene products GENES Glycoside Hydrolases - biosynthesis Glycoside Hydrolases - genetics Glycoside Hydrolases - isolation & purification Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization PARED CELULAR PAROI CELLULAIRE PLANTAS PLANTE Restriction Mapping SUCRES Xylan Endo-1,3-beta-Xylosidase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!