Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin
URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-sterile maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-06, Vol.86 (12), p.4435-4439 |
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| creator | Braun, C.J Siedow, J.N Williams, M.E Levings, C.S. III |
| description | URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-sterile maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the bacterium. We show by ion uptake studies in E. coli that a pathotoxin-URF13 interaction causes membrane permeability. Similarly, mitochondrial dysfunction caused by membrane permeabilization probably accounts for increased colonization of maize carrying the Texas cytoplasm by toxinproducing pathogens. Site-directed mutagenesis studies show that approximately one-quarter of the amino acids at the carboxyl end of URF13 can be eliminated without affecting toxin sensitivity. We have identified two dicyclohexylcarbodiimide (DCCD) binding sites in the URF13 protein and show that one of the sites is involved in conferring DCCD protection against the pathotoxin. Substitutional mutations at this DCCD binding site also eliminate toxin sensitivity. |
| doi_str_mv | 10.1073/pnas.86.12.4435 |
| format | Article |
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III</creator><creatorcontrib>Braun, C.J ; Siedow, J.N ; Williams, M.E ; Levings, C.S. III</creatorcontrib><description>URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-sterile maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the bacterium. We show by ion uptake studies in E. coli that a pathotoxin-URF13 interaction causes membrane permeability. Similarly, mitochondrial dysfunction caused by membrane permeabilization probably accounts for increased colonization of maize carrying the Texas cytoplasm by toxinproducing pathogens. Site-directed mutagenesis studies show that approximately one-quarter of the amino acids at the carboxyl end of URF13 can be eliminated without affecting toxin sensitivity. We have identified two dicyclohexylcarbodiimide (DCCD) binding sites in the URF13 protein and show that one of the sites is involved in conferring DCCD protection against the pathotoxin. Substitutional mutations at this DCCD binding site also eliminate toxin sensitivity.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.12.4435</identifier><identifier>PMID: 2660137</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Amino acids ; Binding sites ; Biological and medical sciences ; Bipolaris maydis ; Cell Membrane - metabolism ; Classical genetics, quantitative genetics, hybrids ; COCHLIOBOLUS ; COCHLIOBOLUS HETEROSTROPHUS ; Codons ; Corn ; Dicyclohexylcarbodiimide - metabolism ; DISEASE RESISTANCE ; DNA, Mitochondrial - genetics ; Escherichia coli - metabolism ; ESTERILIDAD MASCULINA ; Fundamental and applied biological sciences. Psychology ; Generalities ; Genes ; Genetic mutation ; Genetic vectors ; Genetics and breeding of economic plants ; Genetics of eukaryotes. Biological and molecular evolution ; Kinetics ; MALE INFERTILITY ; Mitochondria ; Mitochondria - metabolism ; Molecular Sequence Data ; MUTACION ; MUTATION ; Mycotoxins - pharmacology ; Pest resistance ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Plants - drug effects ; Plants - genetics ; Protein Binding ; Protein Conformation ; Pteridophyta, spermatophyta ; Recombinant Proteins - metabolism ; RESISTANCE AUX MALADIES ; RESISTENCIA A LA ENFERMEDAD ; Respiration ; STERILITE MALE ; Toxins ; Varietal selection. Specialized plant breeding, plant breeding aims ; Vegetals ; ZEA MAYS ; Zea mays - drug effects ; Zea mays - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-06, Vol.86 (12), p.4435-4439</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-66cd6498344b3579572d4c2e823d9f3f16198066d0df63c62f2e5f072ab6bc073</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/33705$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/33705$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7333768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2660137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Braun, C.J</creatorcontrib><creatorcontrib>Siedow, J.N</creatorcontrib><creatorcontrib>Williams, M.E</creatorcontrib><creatorcontrib>Levings, C.S. III</creatorcontrib><title>Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-sterile maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the bacterium. We show by ion uptake studies in E. coli that a pathotoxin-URF13 interaction causes membrane permeability. Similarly, mitochondrial dysfunction caused by membrane permeabilization probably accounts for increased colonization of maize carrying the Texas cytoplasm by toxinproducing pathogens. Site-directed mutagenesis studies show that approximately one-quarter of the amino acids at the carboxyl end of URF13 can be eliminated without affecting toxin sensitivity. We have identified two dicyclohexylcarbodiimide (DCCD) binding sites in the URF13 protein and show that one of the sites is involved in conferring DCCD protection against the pathotoxin. Substitutional mutations at this DCCD binding site also eliminate toxin sensitivity.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Bipolaris maydis</subject><subject>Cell Membrane - metabolism</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>COCHLIOBOLUS</subject><subject>COCHLIOBOLUS HETEROSTROPHUS</subject><subject>Codons</subject><subject>Corn</subject><subject>Dicyclohexylcarbodiimide - metabolism</subject><subject>DISEASE RESISTANCE</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>ESTERILIDAD MASCULINA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Genetic vectors</subject><subject>Genetics and breeding of economic plants</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Kinetics</subject><subject>MALE INFERTILITY</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Molecular Sequence Data</subject><subject>MUTACION</subject><subject>MUTATION</subject><subject>Mycotoxins - pharmacology</subject><subject>Pest resistance</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Plants - drug effects</subject><subject>Plants - genetics</subject><subject>Protein Binding</subject><subject>Protein Conformation</subject><subject>Pteridophyta, spermatophyta</subject><subject>Recombinant Proteins - metabolism</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>Respiration</subject><subject>STERILITE MALE</subject><subject>Toxins</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>Vegetals</subject><subject>ZEA MAYS</subject><subject>Zea mays - drug effects</subject><subject>Zea mays - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1vFDEUxC0ECkegRkICuUCk2ou_1vYWFCgCghREQdJi-bz2naNd-7C9UcJfj0-3HEmTxi7mN--N3gDwGqMlRoKeboPOS8mXmCwZo-0TsMCoww1nHXoKFggR0UhG2HPwIudrhFDXSnQEjgjnCFOxAL--T0UXH0OGPsCysXDU_k99fYlmE0OfvB7gZTMlhylc22ChHfzogy4WZhuyL_7GlztYItTQTWFd8a0um1jirQ8vwTOnh2xfzf8xuPry-fLsvLn48fXb2aeLxrQMlYZz09fIkjK2oq3oWkF6ZoiVhPadow5z3EnEeY96x6nhxBHbOiSIXvGVqXc4Bh_3c7fTarS9saEkPaht8qNOdypqrx4qwW_UOt4oIgWRrPo_zP4Uf082FzX6bOww6GDjlBVuKe8wRhU83YMmxZyTdYcdGKldI2rXiJJcYaJ2jVTH2_vRDvxcQdXfz7rORg8u6WB8PmCCUiq4rNjJjO3m_1P_71FuGoZib0sl3z1KVuDNHrjOJaYDUfege6LTUel1qlGufsqOcIEx_QvIo76K</recordid><startdate>19890601</startdate><enddate>19890601</enddate><creator>Braun, C.J</creator><creator>Siedow, J.N</creator><creator>Williams, M.E</creator><creator>Levings, C.S. III</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19890601</creationdate><title>Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin</title><author>Braun, C.J ; Siedow, J.N ; Williams, M.E ; Levings, C.S. III</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-66cd6498344b3579572d4c2e823d9f3f16198066d0df63c62f2e5f072ab6bc073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Bipolaris maydis</topic><topic>Cell Membrane - metabolism</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>COCHLIOBOLUS</topic><topic>COCHLIOBOLUS HETEROSTROPHUS</topic><topic>Codons</topic><topic>Corn</topic><topic>Dicyclohexylcarbodiimide - metabolism</topic><topic>DISEASE RESISTANCE</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>ESTERILIDAD MASCULINA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities</topic><topic>Genes</topic><topic>Genetic mutation</topic><topic>Genetic vectors</topic><topic>Genetics and breeding of economic plants</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Kinetics</topic><topic>MALE INFERTILITY</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Molecular Sequence Data</topic><topic>MUTACION</topic><topic>MUTATION</topic><topic>Mycotoxins - pharmacology</topic><topic>Pest resistance</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Plants - drug effects</topic><topic>Plants - genetics</topic><topic>Protein Binding</topic><topic>Protein Conformation</topic><topic>Pteridophyta, spermatophyta</topic><topic>Recombinant Proteins - metabolism</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>Respiration</topic><topic>STERILITE MALE</topic><topic>Toxins</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Vegetals</topic><topic>ZEA MAYS</topic><topic>Zea mays - drug effects</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braun, C.J</creatorcontrib><creatorcontrib>Siedow, J.N</creatorcontrib><creatorcontrib>Williams, M.E</creatorcontrib><creatorcontrib>Levings, C.S. 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III</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-06-01</date><risdate>1989</risdate><volume>86</volume><issue>12</issue><spage>4435</spage><epage>4439</epage><pages>4435-4439</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-sterile maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the bacterium. We show by ion uptake studies in E. coli that a pathotoxin-URF13 interaction causes membrane permeability. Similarly, mitochondrial dysfunction caused by membrane permeabilization probably accounts for increased colonization of maize carrying the Texas cytoplasm by toxinproducing pathogens. Site-directed mutagenesis studies show that approximately one-quarter of the amino acids at the carboxyl end of URF13 can be eliminated without affecting toxin sensitivity. We have identified two dicyclohexylcarbodiimide (DCCD) binding sites in the URF13 protein and show that one of the sites is involved in conferring DCCD protection against the pathotoxin. Substitutional mutations at this DCCD binding site also eliminate toxin sensitivity.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2660137</pmid><doi>10.1073/pnas.86.12.4435</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence Amino acids Binding sites Biological and medical sciences Bipolaris maydis Cell Membrane - metabolism Classical genetics, quantitative genetics, hybrids COCHLIOBOLUS COCHLIOBOLUS HETEROSTROPHUS Codons Corn Dicyclohexylcarbodiimide - metabolism DISEASE RESISTANCE DNA, Mitochondrial - genetics Escherichia coli - metabolism ESTERILIDAD MASCULINA Fundamental and applied biological sciences. Psychology Generalities Genes Genetic mutation Genetic vectors Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Kinetics MALE INFERTILITY Mitochondria Mitochondria - metabolism Molecular Sequence Data MUTACION MUTATION Mycotoxins - pharmacology Pest resistance Plant Proteins - genetics Plant Proteins - metabolism Plants Plants - drug effects Plants - genetics Protein Binding Protein Conformation Pteridophyta, spermatophyta Recombinant Proteins - metabolism RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD Respiration STERILITE MALE Toxins Varietal selection. Specialized plant breeding, plant breeding aims Vegetals ZEA MAYS Zea mays - drug effects Zea mays - genetics |
| title | Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin |
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