Development, validation and biomedical applications of stable-isotope dilution GC–MS and GC–MS/MS techniques for circulating malondialdehyde (MDA) after pentafluorobenzyl bromide derivatization: MDA as a biomarker of oxidative stress and its relation to 15(S)-8-iso-prostaglandin F2α and nitric oxide (NO)

•Malondialdehyde (MDA) is a popular oxidative stress biomarker.•We report a unique derivatization of MDA with pentafluorobenzyl bromide.•Deuterium-labelled MDA is used as the internal standard.•MDA is quantitated in human plasma and urine by GC–MS and GC–MS/MS.•Several biomedical applications of the...

Ausführliche Beschreibung