CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme

The CRISPR/Cas9 system is rapidly becoming the reagent of choice for targeted mutagenesis and gene editing in crop species. There are currently intense research efforts in the crop sciences to identify efficient CRISPR/Cas9 platforms to carry out targeted mutagenesis and gene editing projects. These...

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Bibliographische Detailangaben
Veröffentlicht in:	GM crops & food 2015-01, Vol.6 (4), p.243-252
Hauptverfasser:	Michno, Jean-Michel, Wang, Xiaobo, Liu, Junqi, Curtin, Shaun J, Kono, Thomas JY, Stupar, Robert M
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites CRISPR-associated endonuclease 9 CRISPR-Associated Proteins - chemistry CRISPR-Associated Proteins - genetics CRISPR-Cas Systems CRISPR/Cas9 crops DNA gene editing genes Genes, Plant Genetic Engineering - methods genetically modified plants genome engineering Glycine max - genetics hairy roots Internet loci Medicago Medicago truncatula Medicago truncatula - genetics Mutagenesis, Site-Directed - methods nucleotide sequences Plants, Genetically Modified Promoter Regions, Genetic Research Papers restriction endonucleases RNA Sequence Analysis, DNA site-directed mutagenesis Software somatic cells soybean soybeans targeted mutagenesis world wide web
Online-Zugang:	Volltext
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