Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line
Berberine is a clinically important natural isoquinoline alkaloid found in many medicinal herbs. Berberine has been shown to have many pharmacological effects including antimicrobial, antitumor, and anti-inflammatory activities. However, the effects and mechanism of action of berberine have not been...
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| Veröffentlicht in: | Oncology research 2015-07, Vol.22 (3), p.147-157 |
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| description | Berberine is a clinically important natural isoquinoline alkaloid found in many medicinal herbs. Berberine has been shown to have many pharmacological effects including antimicrobial, antitumor, and anti-inflammatory activities. However, the effects and mechanism of action of berberine
have not been studied in chondrosarcoma. Therefore, the effects of berberine on proliferation in a human chondrosarcoma cell line (HTB-94) were investigated. Berberine inhibited cell proliferation in a concentration-dependent manner. We also determined that inhibition of cell proliferation
by berberine occurred via G2/M phase arrest in HTB-94 cells. Berberine induced cell cycle arrest at the G2/M phase by upregulation of p53 and p21 expression and suppressed cyclin B1, cyclin-dependent kinase 1 (cdc2), cdc25c, and phosphorylated retinoblastoma tumor-suppressor
protein (pRb) expression. In addition, berberine stimulated phosphorylation of protein kinase B (Akt) and p38 kinase. Inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt with LY294002 (LY) and p38 kinase with SB203580 (SB), respectively, decreased berberine-induced p53 and p21 expression
and restored cell proliferation and expression of cyclin B1, cdc2, cdc25c, and pRb cell cycle progression proteins. These results suggest that berberine-induced inhibition of cell proliferation by cell cycle arrest at the G2/M phases was regulated through PI3K/Akt and p38 kinase
pathways in HTB-94 chondrosarcoma cells. |
| doi_str_mv | 10.3727/096504015X14298122915583 |
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| fullrecord | <record><control><sourceid>pubtec_pubme</sourceid><recordid>TN_cdi_pubmed_primary_26168133</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ingid>cog/or/2014/00000022/00000003/art00003</ingid><sourcerecordid>cog/or/2014/00000022/00000003/art00003</sourcerecordid><originalsourceid>FETCH-LOGICAL-i4223-2cad7716c8b9d703f03a508912555dfb238248d378d688091d1310c24bb47be63</originalsourceid><addsrcrecordid>eNplkdFu0zAUhi0EYmXwCsgvEGqf48TODVKp2FbRiV0MiTvLiZ3OJbUrJ6m0Pf28rRtD-OYc6Xz-dH6bEMrZF5Qg56yuSiYYL39zAbXiADUvS4VvyCzXssCaVW_J7AErMidPyIdh2DIGQor6PTmBileKI87IdhXs1I4-Bho7eg7zS7pIyQ0jbW7pN5cal3xw9OANXWTsYJ7RqxX-mC_-jNQES_eoqA_0YtqZQJc3MdgUB5PauDN06fqerrPkI3nXmX5wn471lPw6-369vCjWP89Xy8W68AIAC2iNlZJXrWpqKxl2DE3JVM0hJ7NdA6hAKItS2UopVnPLkbMWRNMI2bgKT8nXJ-9-anbOti6MyfR6n_zOpFsdjdf_ToK_0Zt40FKhEiVmwefXgpebz6-WgcsnwIdNdhi9jVMKOZP2rW7jRh__R-oDQEANDDhTnGnORKmt68zUj3o0SW_u9IB_N_7P9yCLKQu40OzxABwbhtqk8bHBe-n3nEg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line</title><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Eo, Seong-Hui ; Kim, Ju-Hee ; Kim, Song-Ja</creator><creatorcontrib>Eo, Seong-Hui ; Kim, Ju-Hee ; Kim, Song-Ja</creatorcontrib><description>Berberine is a clinically important natural isoquinoline alkaloid found in many medicinal herbs. Berberine has been shown to have many pharmacological effects including antimicrobial, antitumor, and anti-inflammatory activities. However, the effects and mechanism of action of berberine
have not been studied in chondrosarcoma. Therefore, the effects of berberine on proliferation in a human chondrosarcoma cell line (HTB-94) were investigated. Berberine inhibited cell proliferation in a concentration-dependent manner. We also determined that inhibition of cell proliferation
by berberine occurred via G2/M phase arrest in HTB-94 cells. Berberine induced cell cycle arrest at the G2/M phase by upregulation of p53 and p21 expression and suppressed cyclin B1, cyclin-dependent kinase 1 (cdc2), cdc25c, and phosphorylated retinoblastoma tumor-suppressor
protein (pRb) expression. In addition, berberine stimulated phosphorylation of protein kinase B (Akt) and p38 kinase. Inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt with LY294002 (LY) and p38 kinase with SB203580 (SB), respectively, decreased berberine-induced p53 and p21 expression
and restored cell proliferation and expression of cyclin B1, cdc2, cdc25c, and pRb cell cycle progression proteins. These results suggest that berberine-induced inhibition of cell proliferation by cell cycle arrest at the G2/M phases was regulated through PI3K/Akt and p38 kinase
pathways in HTB-94 chondrosarcoma cells.</description><identifier>ISSN: 0965-0407</identifier><identifier>EISSN: 1555-3906</identifier><identifier>DOI: 10.3727/096504015X14298122915583</identifier><identifier>PMID: 26168133</identifier><language>eng</language><publisher>Elmsford, NY: Cognizant Communication Corporation</publisher><subject>Berberine ; Berberine - pharmacology ; CDC2 Protein Kinase ; cdc25 Phosphatases - biosynthesis ; Cell Cycle Arrest ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Chondrosarcoma ; Chondrosarcoma - drug therapy ; Chondrosarcoma - genetics ; Chromones - pharmacology ; Cyclin B1 - biosynthesis ; Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis ; Cyclin-Dependent Kinases - biosynthesis ; Enzyme Activation - drug effects ; G2 Phase Cell Cycle Checkpoints - drug effects ; Humans ; Imidazoles - pharmacology ; Morpholines - pharmacology ; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors ; p38 Mitogen-Activated Protein Kinases - metabolism ; Phosphatidylinositol 3-Kinases - metabolism ; Phosphoinositide-3 Kinase Inhibitors ; Phosphorylation - drug effects ; Proliferation ; Proto-Oncogene Proteins c-akt - antagonists & inhibitors ; Proto-Oncogene Proteins c-akt - metabolism ; Pyridines - pharmacology ; Retinoblastoma Protein - biosynthesis ; Retinoblastoma Protein - metabolism ; Tumor Suppressor Protein p53 - biosynthesis ; Up-Regulation - drug effects</subject><ispartof>Oncology research, 2015-07, Vol.22 (3), p.147-157</ispartof><rights>Copyright © 2015 Cognizant Comm. Corp. 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838453/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838453/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,288,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26168133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eo, Seong-Hui</creatorcontrib><creatorcontrib>Kim, Ju-Hee</creatorcontrib><creatorcontrib>Kim, Song-Ja</creatorcontrib><title>Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line</title><title>Oncology research</title><addtitle>Oncol Res</addtitle><description>Berberine is a clinically important natural isoquinoline alkaloid found in many medicinal herbs. Berberine has been shown to have many pharmacological effects including antimicrobial, antitumor, and anti-inflammatory activities. However, the effects and mechanism of action of berberine
have not been studied in chondrosarcoma. Therefore, the effects of berberine on proliferation in a human chondrosarcoma cell line (HTB-94) were investigated. Berberine inhibited cell proliferation in a concentration-dependent manner. We also determined that inhibition of cell proliferation
by berberine occurred via G2/M phase arrest in HTB-94 cells. Berberine induced cell cycle arrest at the G2/M phase by upregulation of p53 and p21 expression and suppressed cyclin B1, cyclin-dependent kinase 1 (cdc2), cdc25c, and phosphorylated retinoblastoma tumor-suppressor
protein (pRb) expression. In addition, berberine stimulated phosphorylation of protein kinase B (Akt) and p38 kinase. Inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt with LY294002 (LY) and p38 kinase with SB203580 (SB), respectively, decreased berberine-induced p53 and p21 expression
and restored cell proliferation and expression of cyclin B1, cdc2, cdc25c, and pRb cell cycle progression proteins. These results suggest that berberine-induced inhibition of cell proliferation by cell cycle arrest at the G2/M phases was regulated through PI3K/Akt and p38 kinase
pathways in HTB-94 chondrosarcoma cells.</description><subject>Berberine</subject><subject>Berberine - pharmacology</subject><subject>CDC2 Protein Kinase</subject><subject>cdc25 Phosphatases - biosynthesis</subject><subject>Cell Cycle Arrest</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Chondrosarcoma</subject><subject>Chondrosarcoma - drug therapy</subject><subject>Chondrosarcoma - genetics</subject><subject>Chromones - pharmacology</subject><subject>Cyclin B1 - biosynthesis</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis</subject><subject>Cyclin-Dependent Kinases - biosynthesis</subject><subject>Enzyme Activation - drug effects</subject><subject>G2 Phase Cell Cycle Checkpoints - drug effects</subject><subject>Humans</subject><subject>Imidazoles - pharmacology</subject><subject>Morpholines - pharmacology</subject><subject>p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Phosphoinositide-3 Kinase Inhibitors</subject><subject>Phosphorylation - drug effects</subject><subject>Proliferation</subject><subject>Proto-Oncogene Proteins c-akt - antagonists & inhibitors</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Pyridines - pharmacology</subject><subject>Retinoblastoma Protein - biosynthesis</subject><subject>Retinoblastoma Protein - metabolism</subject><subject>Tumor Suppressor Protein p53 - biosynthesis</subject><subject>Up-Regulation - drug effects</subject><issn>0965-0407</issn><issn>1555-3906</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkdFu0zAUhi0EYmXwCsgvEGqf48TODVKp2FbRiV0MiTvLiZ3OJbUrJ6m0Pf28rRtD-OYc6Xz-dH6bEMrZF5Qg56yuSiYYL39zAbXiADUvS4VvyCzXssCaVW_J7AErMidPyIdh2DIGQor6PTmBileKI87IdhXs1I4-Bho7eg7zS7pIyQ0jbW7pN5cal3xw9OANXWTsYJ7RqxX-mC_-jNQES_eoqA_0YtqZQJc3MdgUB5PauDN06fqerrPkI3nXmX5wn471lPw6-369vCjWP89Xy8W68AIAC2iNlZJXrWpqKxl2DE3JVM0hJ7NdA6hAKItS2UopVnPLkbMWRNMI2bgKT8nXJ-9-anbOti6MyfR6n_zOpFsdjdf_ToK_0Zt40FKhEiVmwefXgpebz6-WgcsnwIdNdhi9jVMKOZP2rW7jRh__R-oDQEANDDhTnGnORKmt68zUj3o0SW_u9IB_N_7P9yCLKQu40OzxABwbhtqk8bHBe-n3nEg</recordid><startdate>20150716</startdate><enddate>20150716</enddate><creator>Eo, Seong-Hui</creator><creator>Kim, Ju-Hee</creator><creator>Kim, Song-Ja</creator><general>Cognizant Communication Corporation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20150716</creationdate><title>Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line</title><author>Eo, Seong-Hui ; Kim, Ju-Hee ; Kim, Song-Ja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4223-2cad7716c8b9d703f03a508912555dfb238248d378d688091d1310c24bb47be63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Berberine</topic><topic>Berberine - pharmacology</topic><topic>CDC2 Protein Kinase</topic><topic>cdc25 Phosphatases - biosynthesis</topic><topic>Cell Cycle Arrest</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Chondrosarcoma</topic><topic>Chondrosarcoma - drug therapy</topic><topic>Chondrosarcoma - genetics</topic><topic>Chromones - pharmacology</topic><topic>Cyclin B1 - biosynthesis</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis</topic><topic>Cyclin-Dependent Kinases - biosynthesis</topic><topic>Enzyme Activation - drug effects</topic><topic>G2 Phase Cell Cycle Checkpoints - drug effects</topic><topic>Humans</topic><topic>Imidazoles - pharmacology</topic><topic>Morpholines - pharmacology</topic><topic>p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Phosphoinositide-3 Kinase Inhibitors</topic><topic>Phosphorylation - drug effects</topic><topic>Proliferation</topic><topic>Proto-Oncogene Proteins c-akt - antagonists & inhibitors</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Pyridines - pharmacology</topic><topic>Retinoblastoma Protein - biosynthesis</topic><topic>Retinoblastoma Protein - metabolism</topic><topic>Tumor Suppressor Protein p53 - biosynthesis</topic><topic>Up-Regulation - drug effects</topic><toplevel>online_resources</toplevel><creatorcontrib>Eo, Seong-Hui</creatorcontrib><creatorcontrib>Kim, Ju-Hee</creatorcontrib><creatorcontrib>Kim, Song-Ja</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eo, Seong-Hui</au><au>Kim, Ju-Hee</au><au>Kim, Song-Ja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line</atitle><jtitle>Oncology research</jtitle><addtitle>Oncol Res</addtitle><date>2015-07-16</date><risdate>2015</risdate><volume>22</volume><issue>3</issue><spage>147</spage><epage>157</epage><pages>147-157</pages><issn>0965-0407</issn><eissn>1555-3906</eissn><abstract>Berberine is a clinically important natural isoquinoline alkaloid found in many medicinal herbs. Berberine has been shown to have many pharmacological effects including antimicrobial, antitumor, and anti-inflammatory activities. However, the effects and mechanism of action of berberine
have not been studied in chondrosarcoma. Therefore, the effects of berberine on proliferation in a human chondrosarcoma cell line (HTB-94) were investigated. Berberine inhibited cell proliferation in a concentration-dependent manner. We also determined that inhibition of cell proliferation
by berberine occurred via G2/M phase arrest in HTB-94 cells. Berberine induced cell cycle arrest at the G2/M phase by upregulation of p53 and p21 expression and suppressed cyclin B1, cyclin-dependent kinase 1 (cdc2), cdc25c, and phosphorylated retinoblastoma tumor-suppressor
protein (pRb) expression. In addition, berberine stimulated phosphorylation of protein kinase B (Akt) and p38 kinase. Inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt with LY294002 (LY) and p38 kinase with SB203580 (SB), respectively, decreased berberine-induced p53 and p21 expression
and restored cell proliferation and expression of cyclin B1, cdc2, cdc25c, and pRb cell cycle progression proteins. These results suggest that berberine-induced inhibition of cell proliferation by cell cycle arrest at the G2/M phases was regulated through PI3K/Akt and p38 kinase
pathways in HTB-94 chondrosarcoma cells.</abstract><cop>Elmsford, NY</cop><pub>Cognizant Communication Corporation</pub><pmid>26168133</pmid><doi>10.3727/096504015X14298122915583</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology research, 2015-07, Vol.22 (3), p.147-157 |
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| source | MEDLINE; IngentaConnect Free/Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Berberine Berberine - pharmacology CDC2 Protein Kinase cdc25 Phosphatases - biosynthesis Cell Cycle Arrest Cell Line, Tumor Cell Proliferation - drug effects Chondrosarcoma Chondrosarcoma - drug therapy Chondrosarcoma - genetics Chromones - pharmacology Cyclin B1 - biosynthesis Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis Cyclin-Dependent Kinases - biosynthesis Enzyme Activation - drug effects G2 Phase Cell Cycle Checkpoints - drug effects Humans Imidazoles - pharmacology Morpholines - pharmacology p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors p38 Mitogen-Activated Protein Kinases - metabolism Phosphatidylinositol 3-Kinases - metabolism Phosphoinositide-3 Kinase Inhibitors Phosphorylation - drug effects Proliferation Proto-Oncogene Proteins c-akt - antagonists & inhibitors Proto-Oncogene Proteins c-akt - metabolism Pyridines - pharmacology Retinoblastoma Protein - biosynthesis Retinoblastoma Protein - metabolism Tumor Suppressor Protein p53 - biosynthesis Up-Regulation - drug effects |
| title | Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line |
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