THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM
This study aims to research the effect of dihydroartemisinin on the proliferation, metastasis and apoptosis in human osteosarcoma cells 143B and the underlying mechanism. This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at con...
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| Veröffentlicht in: | Journal of biological regulators and homeostatic agents 2015-04, Vol.29 (2), p.335 |
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| creator | Liu, W Wang, D W Yu, S Y Cao, Y Yang, L E, X Q Yao, G J Bi, Z G |
| description | This study aims to research the effect of dihydroartemisinin on the proliferation, metastasis and apoptosis in human osteosarcoma cells 143B and the underlying mechanism. This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at concentrations of 15, 25, 35 μmol.L-1 respectively. Experiments including methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and scratch test were carried out. In addition, SPSS 18.0 software from IBM was used for statistical analysis and all the data obtained from the experiments were expressed as mean ± SD, and variance was used to compare the difference between the groups. DHA is proved to be able to inhibit the proliferation and metastasis of osteosarcoma cells, as well as leaving a positive effect on apoptosis in the cytomorphosis. It achieves regulation over the human osteosarcoma cells by keeping the expression of related protein under control. |
| format | Article |
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This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at concentrations of 15, 25, 35 μmol.L-1 respectively. Experiments including methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and scratch test were carried out. In addition, SPSS 18.0 software from IBM was used for statistical analysis and all the data obtained from the experiments were expressed as mean ± SD, and variance was used to compare the difference between the groups. DHA is proved to be able to inhibit the proliferation and metastasis of osteosarcoma cells, as well as leaving a positive effect on apoptosis in the cytomorphosis. It achieves regulation over the human osteosarcoma cells by keeping the expression of related protein under control.</description><identifier>ISSN: 0393-974X</identifier><identifier>PMID: 26122221</identifier><language>eng</language><publisher>Italy</publisher><subject>Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis - drug effects ; Artemisinins - pharmacology ; Bisbenzimidazole ; Bone Neoplasms - pathology ; Cell Line, Tumor ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Coloring Agents ; Dimethyl Sulfoxide - pharmacology ; Docosahexaenoic Acids - pharmacology ; Drug Screening Assays, Antitumor ; Gene Expression Regulation, Neoplastic - drug effects ; Genes, Reporter ; Humans ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Osteosarcoma - pathology ; Tetrazolium Salts ; Thiazoles ; Tumor Stem Cell Assay</subject><ispartof>Journal of biological regulators and homeostatic agents, 2015-04, Vol.29 (2), p.335</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26122221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, W</creatorcontrib><creatorcontrib>Wang, D W</creatorcontrib><creatorcontrib>Yu, S Y</creatorcontrib><creatorcontrib>Cao, Y</creatorcontrib><creatorcontrib>Yang, L</creatorcontrib><creatorcontrib>E, X Q</creatorcontrib><creatorcontrib>Yao, G J</creatorcontrib><creatorcontrib>Bi, Z G</creatorcontrib><title>THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM</title><title>Journal of biological regulators and homeostatic agents</title><addtitle>J Biol Regul Homeost Agents</addtitle><description>This study aims to research the effect of dihydroartemisinin on the proliferation, metastasis and apoptosis in human osteosarcoma cells 143B and the underlying mechanism. This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at concentrations of 15, 25, 35 μmol.L-1 respectively. Experiments including methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and scratch test were carried out. In addition, SPSS 18.0 software from IBM was used for statistical analysis and all the data obtained from the experiments were expressed as mean ± SD, and variance was used to compare the difference between the groups. DHA is proved to be able to inhibit the proliferation and metastasis of osteosarcoma cells, as well as leaving a positive effect on apoptosis in the cytomorphosis. It achieves regulation over the human osteosarcoma cells by keeping the expression of related protein under control.</description><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Artemisinins - pharmacology</subject><subject>Bisbenzimidazole</subject><subject>Bone Neoplasms - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Coloring Agents</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Docosahexaenoic Acids - pharmacology</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Osteosarcoma - pathology</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Tumor Stem Cell Assay</subject><issn>0393-974X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kN9KwzAYxXOhuDl9BckDWMi_Je1laFMbaJPSZKBXo1lbUByUVS-88N3N2Dx88HHg_M7FuQFrRDOaZIK9rsD9snwgxCgT4g6sCMckCq_Br68UVGWpcg9tCQtdvRWdlZ1XjXbaaAOtgedM29lal6qTXlvzDBvlpYunHZSmgLK1rbdnF0uqXSMj57yyTna5bSTMVV1fktq7COeVNNo1D-B26j-X8fH6N2BXKp9XSW1fdC7rZCYYfyVkCqynY08RFgFlAw043U4BYcYFz_hARDpRwg8UjYEEwscgJsFSfsiGLQ6R24CnS-_8HY7jsJ9P78f-9LP_34H-Afp0Tmc</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Liu, W</creator><creator>Wang, D W</creator><creator>Yu, S Y</creator><creator>Cao, Y</creator><creator>Yang, L</creator><creator>E, X Q</creator><creator>Yao, G J</creator><creator>Bi, Z G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201504</creationdate><title>THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM</title><author>Liu, W ; Wang, D W ; Yu, S Y ; Cao, Y ; Yang, L ; E, X Q ; Yao, G J ; Bi, Z G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-2fb4a3ea3017b09d3b185fb01467696d278f326c30eb2b26eb7f7486c9d51bea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Artemisinins - pharmacology</topic><topic>Bisbenzimidazole</topic><topic>Bone Neoplasms - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Coloring Agents</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>Docosahexaenoic Acids - pharmacology</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Osteosarcoma - pathology</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Tumor Stem Cell Assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, W</creatorcontrib><creatorcontrib>Wang, D W</creatorcontrib><creatorcontrib>Yu, S Y</creatorcontrib><creatorcontrib>Cao, Y</creatorcontrib><creatorcontrib>Yang, L</creatorcontrib><creatorcontrib>E, X Q</creatorcontrib><creatorcontrib>Yao, G J</creatorcontrib><creatorcontrib>Bi, Z G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of biological regulators and homeostatic agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, W</au><au>Wang, D W</au><au>Yu, S Y</au><au>Cao, Y</au><au>Yang, L</au><au>E, X Q</au><au>Yao, G J</au><au>Bi, Z G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM</atitle><jtitle>Journal of biological regulators and homeostatic agents</jtitle><addtitle>J Biol Regul Homeost Agents</addtitle><date>2015-04</date><risdate>2015</risdate><volume>29</volume><issue>2</issue><spage>335</spage><pages>335-</pages><issn>0393-974X</issn><abstract>This study aims to research the effect of dihydroartemisinin on the proliferation, metastasis and apoptosis in human osteosarcoma cells 143B and the underlying mechanism. This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at concentrations of 15, 25, 35 μmol.L-1 respectively. Experiments including methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and scratch test were carried out. In addition, SPSS 18.0 software from IBM was used for statistical analysis and all the data obtained from the experiments were expressed as mean ± SD, and variance was used to compare the difference between the groups. DHA is proved to be able to inhibit the proliferation and metastasis of osteosarcoma cells, as well as leaving a positive effect on apoptosis in the cytomorphosis. It achieves regulation over the human osteosarcoma cells by keeping the expression of related protein under control.</abstract><cop>Italy</cop><pmid>26122221</pmid></addata></record> |
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| ispartof | Journal of biological regulators and homeostatic agents, 2015-04, Vol.29 (2), p.335 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Antineoplastic Agents, Phytogenic - pharmacology Apoptosis - drug effects Artemisinins - pharmacology Bisbenzimidazole Bone Neoplasms - pathology Cell Line, Tumor Cell Movement - drug effects Cell Proliferation - drug effects Coloring Agents Dimethyl Sulfoxide - pharmacology Docosahexaenoic Acids - pharmacology Drug Screening Assays, Antitumor Gene Expression Regulation, Neoplastic - drug effects Genes, Reporter Humans Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Osteosarcoma - pathology Tetrazolium Salts Thiazoles Tumor Stem Cell Assay |
| title | THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM |
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