Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection...
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| Veröffentlicht in: | Journal of breath research 2015-05, Vol.9 (2), p.027107-027107 |
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| container_title | Journal of breath research |
| container_volume | 9 |
| creator | Greguš, Michal Foret, František Kindlová, Dagmar Pokojová, Eva Plutinský, Marek Doubková, Martina Merta, Zden k Binková, Ilona Sk i ková, Jana Kubá, Petr |
| description | The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD |
| doi_str_mv | 10.1088/1752-7155/9/2/027107 |
| format | Article |
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A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD <1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.</description><identifier>ISSN: 1752-7155</identifier><identifier>ISSN: 1752-7163</identifier><identifier>EISSN: 1752-7163</identifier><identifier>DOI: 10.1088/1752-7155/9/2/027107</identifier><identifier>PMID: 25944821</identifier><identifier>CODEN: JBROBW</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Acetates - analysis ; Acetates - metabolism ; Adult ; Aged ; Ammonium Compounds - analysis ; Ammonium Compounds - metabolism ; anions and cations ; Asthma - metabolism ; Breath Tests - instrumentation ; Breath Tests - methods ; Butyrates - analysis ; Butyrates - metabolism ; Calcium - analysis ; Calcium - metabolism ; Capillary electrophoresis ; Chlorides - analysis ; Chlorides - metabolism ; contactless conductivity detection ; Crown Ethers ; Cystic Fibrosis - metabolism ; Electrophoresis, Capillary ; exhaled breath condensate ; Female ; Humans ; Hydrogen-Ion Concentration ; Ions ; Lactic Acid - analysis ; Lactic Acid - metabolism ; Magnesium - analysis ; Magnesium - metabolism ; Male ; Middle Aged ; Nitrates - analysis ; Nitrates - metabolism ; Nitrites - analysis ; Nitrites - metabolism ; Potassium - analysis ; Potassium - metabolism ; Propionates - analysis ; Propionates - metabolism ; Pulmonary Disease, Chronic Obstructive - metabolism ; Pulmonary Fibrosis - metabolism ; Respiratory diseases ; Sarcoidosis, Pulmonary - metabolism ; Sodium - analysis ; Sodium - metabolism ; Sulfates - analysis ; Sulfates - metabolism</subject><ispartof>Journal of breath research, 2015-05, Vol.9 (2), p.027107-027107</ispartof><rights>2015 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-dd22c1d1dc23f2d8e15969a528cb3c28c17900079ecd8b20428efc53bdb024913</citedby><cites>FETCH-LOGICAL-c376t-dd22c1d1dc23f2d8e15969a528cb3c28c17900079ecd8b20428efc53bdb024913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,776,780,27903,27904,53824,53871</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25944821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Greguš, Michal</creatorcontrib><creatorcontrib>Foret, František</creatorcontrib><creatorcontrib>Kindlová, Dagmar</creatorcontrib><creatorcontrib>Pokojová, Eva</creatorcontrib><creatorcontrib>Plutinský, Marek</creatorcontrib><creatorcontrib>Doubková, Martina</creatorcontrib><creatorcontrib>Merta, Zden k</creatorcontrib><creatorcontrib>Binková, Ilona</creatorcontrib><creatorcontrib>Sk i ková, Jana</creatorcontrib><creatorcontrib>Kubá, Petr</creatorcontrib><title>Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection</title><title>Journal of breath research</title><addtitle>JBR</addtitle><addtitle>J. Breath Res</addtitle><description>The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD <1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.</description><subject>Acetates - analysis</subject><subject>Acetates - metabolism</subject><subject>Adult</subject><subject>Aged</subject><subject>Ammonium Compounds - analysis</subject><subject>Ammonium Compounds - metabolism</subject><subject>anions and cations</subject><subject>Asthma - metabolism</subject><subject>Breath Tests - instrumentation</subject><subject>Breath Tests - methods</subject><subject>Butyrates - analysis</subject><subject>Butyrates - metabolism</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Capillary electrophoresis</subject><subject>Chlorides - analysis</subject><subject>Chlorides - metabolism</subject><subject>contactless conductivity detection</subject><subject>Crown Ethers</subject><subject>Cystic Fibrosis - metabolism</subject><subject>Electrophoresis, Capillary</subject><subject>exhaled breath condensate</subject><subject>Female</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ions</subject><subject>Lactic Acid - analysis</subject><subject>Lactic Acid - metabolism</subject><subject>Magnesium - analysis</subject><subject>Magnesium - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nitrates - analysis</subject><subject>Nitrates - metabolism</subject><subject>Nitrites - analysis</subject><subject>Nitrites - metabolism</subject><subject>Potassium - analysis</subject><subject>Potassium - metabolism</subject><subject>Propionates - analysis</subject><subject>Propionates - metabolism</subject><subject>Pulmonary Disease, Chronic Obstructive - metabolism</subject><subject>Pulmonary Fibrosis - metabolism</subject><subject>Respiratory diseases</subject><subject>Sarcoidosis, Pulmonary - metabolism</subject><subject>Sodium - analysis</subject><subject>Sodium - metabolism</subject><subject>Sulfates - analysis</subject><subject>Sulfates - metabolism</subject><issn>1752-7155</issn><issn>1752-7163</issn><issn>1752-7163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1O3DAUha0KVCjtGyBkiU0307GdOLGXCPVPomJD15Zj33SMMnGwHSgv02ftnWYKEgs2_rn-7vGxDyGnnH3iTKk1b6VYtVzKtV6LNRMtZ-0bcrwvN9XB01rKI_Iu51vGmpop_ZYcCanrWgl-TP78iGMoMYXxFy0boAG3jro4FhgLjT2F3xs7gKddAls2uxMPY7YF0ZHe2xTinGmCPIVkUeeR-pDBZsi0e6TOTmEYLFZhAFdSnDYR2ZDpQ1jEinVlgJz_Cc-uhPtQUAMK4ujlPTns7ZDhw34-IT-_fL65_La6uv76_fLiauWqtikr74Vw3HPvRNULr4BL3WgrhXJd5XDkrWaMtRqcV51gtVDQO1l1vmOi1rw6IR8X3SnFuxlyMduQHaD3EfCBhjeKcSUkF4iev0Bv45xGdGdEW1Ws0ZIrpOqFcinmnKA3Uwpb_AnDmdnlZ3bhmF04Rhthlvyw7WwvPndb8E9N_wNDgC1AiNPzxa9q_gUr-aiG</recordid><startdate>20150506</startdate><enddate>20150506</enddate><creator>Greguš, Michal</creator><creator>Foret, František</creator><creator>Kindlová, Dagmar</creator><creator>Pokojová, Eva</creator><creator>Plutinský, Marek</creator><creator>Doubková, Martina</creator><creator>Merta, Zden k</creator><creator>Binková, Ilona</creator><creator>Sk i ková, Jana</creator><creator>Kubá, Petr</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20150506</creationdate><title>Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection</title><author>Greguš, Michal ; Foret, František ; Kindlová, Dagmar ; Pokojová, Eva ; Plutinský, Marek ; Doubková, Martina ; Merta, Zden k ; Binková, Ilona ; Sk i ková, Jana ; Kubá, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-dd22c1d1dc23f2d8e15969a528cb3c28c17900079ecd8b20428efc53bdb024913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acetates - analysis</topic><topic>Acetates - metabolism</topic><topic>Adult</topic><topic>Aged</topic><topic>Ammonium Compounds - analysis</topic><topic>Ammonium Compounds - metabolism</topic><topic>anions and cations</topic><topic>Asthma - metabolism</topic><topic>Breath Tests - instrumentation</topic><topic>Breath Tests - methods</topic><topic>Butyrates - analysis</topic><topic>Butyrates - metabolism</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Capillary electrophoresis</topic><topic>Chlorides - analysis</topic><topic>Chlorides - metabolism</topic><topic>contactless conductivity detection</topic><topic>Crown Ethers</topic><topic>Cystic Fibrosis - metabolism</topic><topic>Electrophoresis, Capillary</topic><topic>exhaled breath condensate</topic><topic>Female</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Ions</topic><topic>Lactic Acid - analysis</topic><topic>Lactic Acid - metabolism</topic><topic>Magnesium - analysis</topic><topic>Magnesium - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nitrates - analysis</topic><topic>Nitrates - metabolism</topic><topic>Nitrites - analysis</topic><topic>Nitrites - metabolism</topic><topic>Potassium - analysis</topic><topic>Potassium - metabolism</topic><topic>Propionates - analysis</topic><topic>Propionates - metabolism</topic><topic>Pulmonary Disease, Chronic Obstructive - metabolism</topic><topic>Pulmonary Fibrosis - metabolism</topic><topic>Respiratory diseases</topic><topic>Sarcoidosis, Pulmonary - metabolism</topic><topic>Sodium - analysis</topic><topic>Sodium - metabolism</topic><topic>Sulfates - analysis</topic><topic>Sulfates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Greguš, Michal</creatorcontrib><creatorcontrib>Foret, František</creatorcontrib><creatorcontrib>Kindlová, Dagmar</creatorcontrib><creatorcontrib>Pokojová, Eva</creatorcontrib><creatorcontrib>Plutinský, Marek</creatorcontrib><creatorcontrib>Doubková, Martina</creatorcontrib><creatorcontrib>Merta, Zden k</creatorcontrib><creatorcontrib>Binková, Ilona</creatorcontrib><creatorcontrib>Sk i ková, Jana</creatorcontrib><creatorcontrib>Kubá, Petr</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of breath research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Greguš, Michal</au><au>Foret, František</au><au>Kindlová, Dagmar</au><au>Pokojová, Eva</au><au>Plutinský, Marek</au><au>Doubková, Martina</au><au>Merta, Zden k</au><au>Binková, Ilona</au><au>Sk i ková, Jana</au><au>Kubá, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection</atitle><jtitle>Journal of breath research</jtitle><stitle>JBR</stitle><addtitle>J. Breath Res</addtitle><date>2015-05-06</date><risdate>2015</risdate><volume>9</volume><issue>2</issue><spage>027107</spage><epage>027107</epage><pages>027107-027107</pages><issn>1752-7155</issn><issn>1752-7163</issn><eissn>1752-7163</eissn><coden>JBROBW</coden><abstract>The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD <1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>25944821</pmid><doi>10.1088/1752-7155/9/2/027107</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of breath research, 2015-05, Vol.9 (2), p.027107-027107 |
| issn | 1752-7155 1752-7163 1752-7163 |
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| source | Institute of Physics Journals; MEDLINE |
| subjects | Acetates - analysis Acetates - metabolism Adult Aged Ammonium Compounds - analysis Ammonium Compounds - metabolism anions and cations Asthma - metabolism Breath Tests - instrumentation Breath Tests - methods Butyrates - analysis Butyrates - metabolism Calcium - analysis Calcium - metabolism Capillary electrophoresis Chlorides - analysis Chlorides - metabolism contactless conductivity detection Crown Ethers Cystic Fibrosis - metabolism Electrophoresis, Capillary exhaled breath condensate Female Humans Hydrogen-Ion Concentration Ions Lactic Acid - analysis Lactic Acid - metabolism Magnesium - analysis Magnesium - metabolism Male Middle Aged Nitrates - analysis Nitrates - metabolism Nitrites - analysis Nitrites - metabolism Potassium - analysis Potassium - metabolism Propionates - analysis Propionates - metabolism Pulmonary Disease, Chronic Obstructive - metabolism Pulmonary Fibrosis - metabolism Respiratory diseases Sarcoidosis, Pulmonary - metabolism Sodium - analysis Sodium - metabolism Sulfates - analysis Sulfates - metabolism |
| title | Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection |
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