Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases
The correlation between histologic grade, an increasingly important measure of prognosis for patients with breast cancer, and tryptophan levels from tissues of 15 breast carcinoma patients was investigated. Changes in the relative content of key native organic biomolecule tryptophan were seen from t...
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Veröffentlicht in: | Journal of biomedical optics 2014-12, Vol.19 (12), p.125002-125002 |
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creator | Sordillo, Laura A Sordillo, Peter P Budansky, Yury Pu, Yang Alfano, Robert R |
description | The correlation between histologic grade, an increasingly important measure of prognosis for patients with breast cancer, and tryptophan levels from tissues of 15 breast carcinoma patients was investigated. Changes in the relative content of key native organic biomolecule tryptophan were seen from the fluorescence spectra of cancerous and paired normal tissues with excitation wavelengths of 280 and 300 nm. Due to a large spectral overlap and matching excitation-emission spectra, fluorescence resonance energy transfer from tryptophan-donor to reduced nicotinamide adenine dinucleotides-acceptor was noted. We used the ratios of fluorescence intensities at their spectral emission peaks, or spectral fingerprint peaks, at 340, 440, and 460 nm. Higher ratios correlated strongly with high histologic grade, while lower-grade tumors had low ratios. Large tumor size also correlated with high ratios, while the number of lymph node metastases, a major factor in staging, was not correlated with tryptophan levels. High histologic grade correlates strongly with increased content of tryptophan in breast cancer tissues and suggests that measurement of tryptophan content may be useful as a part of the evaluation of these patients. |
doi_str_mv | 10.1117/1.JBO.19.12.125002 |
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Changes in the relative content of key native organic biomolecule tryptophan were seen from the fluorescence spectra of cancerous and paired normal tissues with excitation wavelengths of 280 and 300 nm. Due to a large spectral overlap and matching excitation-emission spectra, fluorescence resonance energy transfer from tryptophan-donor to reduced nicotinamide adenine dinucleotides-acceptor was noted. We used the ratios of fluorescence intensities at their spectral emission peaks, or spectral fingerprint peaks, at 340, 440, and 460 nm. Higher ratios correlated strongly with high histologic grade, while lower-grade tumors had low ratios. Large tumor size also correlated with high ratios, while the number of lymph node metastases, a major factor in staging, was not correlated with tryptophan levels. High histologic grade correlates strongly with increased content of tryptophan in breast cancer tissues and suggests that measurement of tryptophan content may be useful as a part of the evaluation of these patients.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.19.12.125002</identifier><identifier>PMID: 25521053</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Aged ; Breast ; Breast Neoplasms - chemistry ; Breast Neoplasms - pathology ; Cancer ; Case-Control Studies ; Correlation ; Female ; Fluorescence ; Humans ; Lymph Nodes - chemistry ; Lymphatic Metastasis ; Middle Aged ; NAD ; Optical Imaging - methods ; Patients ; Quality ; Spectra ; Spectrometry, Fluorescence - methods ; Tryptophan ; Tryptophan - analysis ; Tryptophan - chemistry ; Tumors</subject><ispartof>Journal of biomedical optics, 2014-12, Vol.19 (12), p.125002-125002</ispartof><rights>2014 Society of Photo-Optical Instrumentation Engineers (SPIE)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-68a7fd4086fdb2eb7b95aa3ccd0fd1916654f947ae0650f7b2030f4de01449dd3</citedby><cites>FETCH-LOGICAL-c500t-68a7fd4086fdb2eb7b95aa3ccd0fd1916654f947ae0650f7b2030f4de01449dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25521053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sordillo, Laura A</creatorcontrib><creatorcontrib>Sordillo, Peter P</creatorcontrib><creatorcontrib>Budansky, Yury</creatorcontrib><creatorcontrib>Pu, Yang</creatorcontrib><creatorcontrib>Alfano, Robert R</creatorcontrib><title>Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>The correlation between histologic grade, an increasingly important measure of prognosis for patients with breast cancer, and tryptophan levels from tissues of 15 breast carcinoma patients was investigated. Changes in the relative content of key native organic biomolecule tryptophan were seen from the fluorescence spectra of cancerous and paired normal tissues with excitation wavelengths of 280 and 300 nm. Due to a large spectral overlap and matching excitation-emission spectra, fluorescence resonance energy transfer from tryptophan-donor to reduced nicotinamide adenine dinucleotides-acceptor was noted. We used the ratios of fluorescence intensities at their spectral emission peaks, or spectral fingerprint peaks, at 340, 440, and 460 nm. Higher ratios correlated strongly with high histologic grade, while lower-grade tumors had low ratios. Large tumor size also correlated with high ratios, while the number of lymph node metastases, a major factor in staging, was not correlated with tryptophan levels. High histologic grade correlates strongly with increased content of tryptophan in breast cancer tissues and suggests that measurement of tryptophan content may be useful as a part of the evaluation of these patients.</description><subject>Aged</subject><subject>Breast</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>Case-Control Studies</subject><subject>Correlation</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Lymph Nodes - chemistry</subject><subject>Lymphatic Metastasis</subject><subject>Middle Aged</subject><subject>NAD</subject><subject>Optical Imaging - methods</subject><subject>Patients</subject><subject>Quality</subject><subject>Spectra</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Tryptophan</subject><subject>Tryptophan - analysis</subject><subject>Tryptophan - chemistry</subject><subject>Tumors</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksFuFSEUhifGxtbqC7gwLN3cW5gZZgZ3tbVq06QuNC4JA4d7aWZgBKbm9ol9DE871Zi00YQEOP_PB_k5RfGK0TVjrD1i6_N3l2sm1qzEwSktnxQHjDd0VZYde4pr2lWrqmm6_eJ5SleU0q4RzbNiv-S8ZJRXB8XPU2ctRPAaEnGe2GEOEZK-LZApBusGFGwMI-kjqJSJVihFkl1KM0pmBpID0VvlNwsiwqCyu8Zy3E05TKgQHXwGn8m1UwQ8xM0OVeUTXv32MZ8O8Q6DxB8ub8nWpRyGsHGabKIyQJQ3JM9jiCS5GyD9nIkPeTEPu3Ha4hZtI2R8s0qQXhR7Vg0JXt7Ph8XXs_dfTj6uLi4_fDo5vlhpDDCvmk611tQYlDV9CX3bC65UpbWh1jDBmobXVtStAtpwatu-pBW1tQHK6loYUx0WbxYuhvcdA8pydBjnMCgPYU6StZRRUbVd939rUwnRcs4ZWsvFqmNIKYKVU3SjijvJqLxtBskkNoNkQrJSLs2Ah17f8-d-BPPnyO_fR8O3xZAmB_IqzNFjNH-Bbtz0AHtXPI7Z6QE-n5491CdjkXz0GPkfj_0FW5Xh4A</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Sordillo, Laura A</creator><creator>Sordillo, Peter P</creator><creator>Budansky, Yury</creator><creator>Pu, Yang</creator><creator>Alfano, Robert R</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20141201</creationdate><title>Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases</title><author>Sordillo, Laura A ; Sordillo, Peter P ; Budansky, Yury ; Pu, Yang ; Alfano, Robert R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-68a7fd4086fdb2eb7b95aa3ccd0fd1916654f947ae0650f7b2030f4de01449dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Breast</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer</topic><topic>Case-Control Studies</topic><topic>Correlation</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Lymph Nodes - chemistry</topic><topic>Lymphatic Metastasis</topic><topic>Middle Aged</topic><topic>NAD</topic><topic>Optical Imaging - methods</topic><topic>Patients</topic><topic>Quality</topic><topic>Spectra</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Tryptophan</topic><topic>Tryptophan - analysis</topic><topic>Tryptophan - chemistry</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sordillo, Laura A</creatorcontrib><creatorcontrib>Sordillo, Peter P</creatorcontrib><creatorcontrib>Budansky, Yury</creatorcontrib><creatorcontrib>Pu, Yang</creatorcontrib><creatorcontrib>Alfano, Robert R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sordillo, Laura A</au><au>Sordillo, Peter P</au><au>Budansky, Yury</au><au>Pu, Yang</au><au>Alfano, Robert R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>19</volume><issue>12</issue><spage>125002</spage><epage>125002</epage><pages>125002-125002</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>The correlation between histologic grade, an increasingly important measure of prognosis for patients with breast cancer, and tryptophan levels from tissues of 15 breast carcinoma patients was investigated. Changes in the relative content of key native organic biomolecule tryptophan were seen from the fluorescence spectra of cancerous and paired normal tissues with excitation wavelengths of 280 and 300 nm. Due to a large spectral overlap and matching excitation-emission spectra, fluorescence resonance energy transfer from tryptophan-donor to reduced nicotinamide adenine dinucleotides-acceptor was noted. We used the ratios of fluorescence intensities at their spectral emission peaks, or spectral fingerprint peaks, at 340, 440, and 460 nm. Higher ratios correlated strongly with high histologic grade, while lower-grade tumors had low ratios. Large tumor size also correlated with high ratios, while the number of lymph node metastases, a major factor in staging, was not correlated with tryptophan levels. High histologic grade correlates strongly with increased content of tryptophan in breast cancer tissues and suggests that measurement of tryptophan content may be useful as a part of the evaluation of these patients.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>25521053</pmid><doi>10.1117/1.JBO.19.12.125002</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Aged Breast Breast Neoplasms - chemistry Breast Neoplasms - pathology Cancer Case-Control Studies Correlation Female Fluorescence Humans Lymph Nodes - chemistry Lymphatic Metastasis Middle Aged NAD Optical Imaging - methods Patients Quality Spectra Spectrometry, Fluorescence - methods Tryptophan Tryptophan - analysis Tryptophan - chemistry Tumors |
title | Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases |
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