GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats
Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemia‑reperfusion (GI‑R) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypot...
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| Veröffentlicht in: | Molecular medicine reports 2015-02, Vol.11 (2), p.1057 |
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| creator | Gao, Lin Zhu, Tao Xie, Guilin Lou, Xiangxin Li, Shibao Zhou, Yan Deng, Zhenxu Chu, Dechang Lou, Jiyu Du, Dongshu |
| description | Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemia‑reperfusion (GI‑R) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (Ad‑GABA(A)R) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABA(A)R treatment in the GI‑R injury rats. Ad‑GABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats. |
| doi_str_mv | 10.3892/mmr.2014.2816 |
| format | Article |
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The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (Ad‑GABA(A)R) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABA(A)R treatment in the GI‑R injury rats. Ad‑GABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats.</description><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2014.2816</identifier><identifier>PMID: 25354809</identifier><language>eng</language><publisher>Greece</publisher><subject>Adenoviridae - genetics ; Angiotensin II - blood ; Animals ; Blood Flow Velocity ; Disease Models, Animal ; Gastric Mucosa - blood supply ; Genetic Vectors - metabolism ; Hypothalamic Area, Lateral - metabolism ; Immunohistochemistry ; Male ; Norepinephrine - blood ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-A - genetics ; Receptors, GABA-A - metabolism ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Splanchnic Nerves - metabolism ; Splanchnic Nerves - pathology ; Stomach - pathology</subject><ispartof>Molecular medicine reports, 2015-02, Vol.11 (2), p.1057</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25354809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Lin</creatorcontrib><creatorcontrib>Zhu, Tao</creatorcontrib><creatorcontrib>Xie, Guilin</creatorcontrib><creatorcontrib>Lou, Xiangxin</creatorcontrib><creatorcontrib>Li, Shibao</creatorcontrib><creatorcontrib>Zhou, Yan</creatorcontrib><creatorcontrib>Deng, Zhenxu</creatorcontrib><creatorcontrib>Chu, Dechang</creatorcontrib><creatorcontrib>Lou, Jiyu</creatorcontrib><creatorcontrib>Du, Dongshu</creatorcontrib><title>GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemia‑reperfusion (GI‑R) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (Ad‑GABA(A)R) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABA(A)R treatment in the GI‑R injury rats. Ad‑GABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats.</description><subject>Adenoviridae - genetics</subject><subject>Angiotensin II - blood</subject><subject>Animals</subject><subject>Blood Flow Velocity</subject><subject>Disease Models, Animal</subject><subject>Gastric Mucosa - blood supply</subject><subject>Genetic Vectors - metabolism</subject><subject>Hypothalamic Area, Lateral - metabolism</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Norepinephrine - blood</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, GABA-A - genetics</subject><subject>Receptors, GABA-A - metabolism</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Splanchnic Nerves - metabolism</subject><subject>Splanchnic Nerves - pathology</subject><subject>Stomach - pathology</subject><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10L1OwzAUBWALCdFSGFmRRxhS_JvaY6igIFVigbm6ca9JqqSJbAeRjVfgFXkSQJTpSOdI33AIueBsLo0VN20b5oJxNReG50dkyheWZ5IxNSGnMe4Yy7XQ9oRMhJZaGWanZFwVt8VVcU0DOuxTF2j3hgHf-4Ax1t2e1nuaKqQNJAzQ0Grsu1RBA23tKAQECinhfviZI32FmMJPX0dXYVvD18dnwB6DHw7UbgjjrxggxTNy7KGJeH7IGXm5v3tePmTrp9XjslhnPVc8ZQryHEB4Z5yzzJQl18wyuZXgJVNGWMVk6UrGvVwYbjVXykMJwhq06LWQM3L55_ZD2eJ204e6hTBu_j-Q369vX5c</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Gao, Lin</creator><creator>Zhu, Tao</creator><creator>Xie, Guilin</creator><creator>Lou, Xiangxin</creator><creator>Li, Shibao</creator><creator>Zhou, Yan</creator><creator>Deng, Zhenxu</creator><creator>Chu, Dechang</creator><creator>Lou, Jiyu</creator><creator>Du, Dongshu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201502</creationdate><title>GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats</title><author>Gao, Lin ; 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The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (Ad‑GABA(A)R) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABA(A)R treatment in the GI‑R injury rats. Ad‑GABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats.</abstract><cop>Greece</cop><pmid>25354809</pmid><doi>10.3892/mmr.2014.2816</doi></addata></record> |
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| ispartof | Molecular medicine reports, 2015-02, Vol.11 (2), p.1057 |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenoviridae - genetics Angiotensin II - blood Animals Blood Flow Velocity Disease Models, Animal Gastric Mucosa - blood supply Genetic Vectors - metabolism Hypothalamic Area, Lateral - metabolism Immunohistochemistry Male Norepinephrine - blood Rats Rats, Sprague-Dawley Receptors, GABA-A - genetics Receptors, GABA-A - metabolism Reperfusion Injury - metabolism Reperfusion Injury - pathology Splanchnic Nerves - metabolism Splanchnic Nerves - pathology Stomach - pathology |
| title | GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats |
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