Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation

Aspirin‐pretreated, 32P‐prelabeled, washed human platelets resuspended in a buffer containing apyrase and 2% plasma were exposed to epinephrine and the Ca2+ ionophore A23187. Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain...

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Veröffentlicht in:	FEBS letters 1989-01, Vol.243 (2), p.275-279
Hauptverfasser:	Olbrich, Christine, Siess, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blood coagulation. Blood cells Ca2+ ionophore Calcimycin - pharmacology Drug Synergism Enzyme Activation - drug effects Epinephrine Epinephrine - pharmacology Fundamental and applied biological sciences. Psychology Humans Molecular and cellular biology Phosphatidic acid Phosphatidic Acids - biosynthesis Phosphorylation Platelet Platelet aggregation Platelet Aggregation - drug effects Protein kinase C Protein Kinase C - metabolism Synergism
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container_title	FEBS letters
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creator	Olbrich, Christine Siess, Wolfgang
description	Aspirin‐pretreated, 32P‐prelabeled, washed human platelets resuspended in a buffer containing apyrase and 2% plasma were exposed to epinephrine and the Ca2+ ionophore A23187. Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G1‐protein activation evoked by epinephrine and Ca2+ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.
doi_str_mv	10.1016/0014-5793(89)80144-9
format	Article
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Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G1‐protein activation evoked by epinephrine and Ca2+ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(89)80144-9</identifier><identifier>PMID: 2492953</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Biological and medical sciences ; Blood coagulation. 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Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G1‐protein activation evoked by epinephrine and Ca2+ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.</description><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Ca2+ ionophore</subject><subject>Calcimycin - pharmacology</subject><subject>Drug Synergism</subject><subject>Enzyme Activation - drug effects</subject><subject>Epinephrine</subject><subject>Epinephrine - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Phosphatidic acid</subject><subject>Phosphatidic Acids - biosynthesis</subject><subject>Phosphorylation</subject><subject>Platelet</subject><subject>Platelet aggregation</subject><subject>Platelet Aggregation - drug effects</subject><subject>Protein kinase C</subject><subject>Protein Kinase C - metabolism</subject><subject>Synergism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkVFPwyAUhYnRzDn9B5rwYqIxVSi0hce5bGqyxBd9JpTSFq1tA8yl_166LfMFcu_5OLmcC8A1Ro8Y4fQJIUyjJOPkjvF7Fgoa8RMwxSwjEaEpOwXTI3IOLpz7QqFmmE_AJKY85gmZgmHZm1b3tQ0nlG0Bfa3hQsYP0HRt19ed1XAek2AK3dBqWxnnjZJNM0DTFhulYd9Irxvtoawqqyvpw0O4Nb7uNh72tvPatPDbtNIFYyiVN7875hKclbJx-upwz8DnavmxeI3W7y9vi_k66nGYMWJJlqaIlDKPY5xinSqasTJXjKW4UBjnSZZpndAc00BwpBKFSUKLRKY5YpkiM3Cz9-03-Y8uRG_Nj7SDOEQQ9NuDLl34WGllq4w7YpjTmBOOA7fac1vT6OFfR2LchhijFmPUgnGx24bgYrV8jkdh7DO-63LyB3cYf0E</recordid><startdate>19890130</startdate><enddate>19890130</enddate><creator>Olbrich, Christine</creator><creator>Siess, Wolfgang</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19890130</creationdate><title>Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation</title><author>Olbrich, Christine ; Siess, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1929-8576603fab22161e6c478fbc8861dc11b577ee54b14b2290c5c1354d5a6b087c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Ca2+ ionophore</topic><topic>Calcimycin - pharmacology</topic><topic>Drug Synergism</topic><topic>Enzyme Activation - drug effects</topic><topic>Epinephrine</topic><topic>Epinephrine - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Phosphatidic acid</topic><topic>Phosphatidic Acids - biosynthesis</topic><topic>Phosphorylation</topic><topic>Platelet</topic><topic>Platelet aggregation</topic><topic>Platelet Aggregation - drug effects</topic><topic>Protein kinase C</topic><topic>Protein Kinase C - metabolism</topic><topic>Synergism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olbrich, Christine</creatorcontrib><creatorcontrib>Siess, Wolfgang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olbrich, Christine</au><au>Siess, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1989-01-30</date><risdate>1989</risdate><volume>243</volume><issue>2</issue><spage>275</spage><epage>279</epage><pages>275-279</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Aspirin‐pretreated, 32P‐prelabeled, washed human platelets resuspended in a buffer containing apyrase and 2% plasma were exposed to epinephrine and the Ca2+ ionophore A23187. Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G1‐protein activation evoked by epinephrine and Ca2+ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>2492953</pmid><doi>10.1016/0014-5793(89)80144-9</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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source	MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects	Biological and medical sciences Blood coagulation. Blood cells Ca2+ ionophore Calcimycin - pharmacology Drug Synergism Enzyme Activation - drug effects Epinephrine Epinephrine - pharmacology Fundamental and applied biological sciences. Psychology Humans Molecular and cellular biology Phosphatidic acid Phosphatidic Acids - biosynthesis Phosphorylation Platelet Platelet aggregation Platelet Aggregation - drug effects Protein kinase C Protein Kinase C - metabolism Synergism
title	Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation
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