The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes
The detection of homocysteine, HCys, was achieved with the use of catechol via 1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence...
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| Veröffentlicht in: | Analyst (London) 2014-01, Vol.139 (15), p.3755-3762 |
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| creator | Lee, P. T Lowinsohn, D Compton, R. G |
| description | The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence and presence of glutathione, cysteine and ascorbic acid using cyclic voltammetry and square wave voltammetry. A calibration curve of homocysteine detection was determined and the sensitivity is (0.20 ± 0.02) μA μM
−1
and the limit of detection is 660 nM within the linear range. Lastly, commercially available multi walled carbon nanotube screen printed electrodes were applied to the system for selective homocysteine detection. This work presents a potential practical application towards medical applications as it can be highly beneficial towards quality healthcare management.
The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. |
| doi_str_mv | 10.1039/c4an00372a |
| format | Article |
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via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence and presence of glutathione, cysteine and ascorbic acid using cyclic voltammetry and square wave voltammetry. A calibration curve of homocysteine detection was determined and the sensitivity is (0.20 ± 0.02) μA μM
−1
and the limit of detection is 660 nM within the linear range. Lastly, commercially available multi walled carbon nanotube screen printed electrodes were applied to the system for selective homocysteine detection. This work presents a potential practical application towards medical applications as it can be highly beneficial towards quality healthcare management.
The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c4an00372a</identifier><identifier>PMID: 24918102</identifier><language>eng</language><publisher>England</publisher><subject>Ascorbic acid ; Ascorbic Acid - analysis ; Calibration ; Carbon ; Carbon - chemistry ; Cysteine ; Cysteine - analysis ; Electrochemical Techniques - methods ; Electrodes ; Glassy carbon ; Glutathione ; Glutathione - analysis ; Homocysteine - analysis ; Limit of Detection ; Nanotubes, Carbon - chemistry ; Voltammetry</subject><ispartof>Analyst (London), 2014-01, Vol.139 (15), p.3755-3762</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-69d8f24bdcf7bd221be1c2d9304e15bfb737815509a913862656d12bfbed6db83</citedby><cites>FETCH-LOGICAL-c434t-69d8f24bdcf7bd221be1c2d9304e15bfb737815509a913862656d12bfbed6db83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2832,2833,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24918102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, P. T</creatorcontrib><creatorcontrib>Lowinsohn, D</creatorcontrib><creatorcontrib>Compton, R. G</creatorcontrib><title>The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence and presence of glutathione, cysteine and ascorbic acid using cyclic voltammetry and square wave voltammetry. A calibration curve of homocysteine detection was determined and the sensitivity is (0.20 ± 0.02) μA μM
−1
and the limit of detection is 660 nM within the linear range. Lastly, commercially available multi walled carbon nanotube screen printed electrodes were applied to the system for selective homocysteine detection. This work presents a potential practical application towards medical applications as it can be highly beneficial towards quality healthcare management.
The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode.</description><subject>Ascorbic acid</subject><subject>Ascorbic Acid - analysis</subject><subject>Calibration</subject><subject>Carbon</subject><subject>Carbon - chemistry</subject><subject>Cysteine</subject><subject>Cysteine - analysis</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Glassy carbon</subject><subject>Glutathione</subject><subject>Glutathione - analysis</subject><subject>Homocysteine - analysis</subject><subject>Limit of Detection</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Voltammetry</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlv2zAUhImiQeM4vfTegrkFQZRy1XI0jGyA0V7cs8DlKWYhiS4pBchvyJ8OvfaWnB4f5-MQmEHoGyU3lPDqpxGqJ4QXTH1CE8pzkUnJys9oQtJtxnIpTtFZjH_TSokkX9ApExUtKWET9LpcAY7QghncM-DtIXizgs4Z1WILw0bxPfYNXvnOm5c4gOsBux4P6ek6QITewEZ_asdBDatEwzU-gNdY9RaraHzQzmBlnMVjdP0TNiroZLz_0kI8RyeNaiN83c8p-nN3u5w_ZIvf94_z2SIzgoshyytbNkxoa5pCW8aoBmqYrTgRQKVudMGLkkpJKlVRXuYpgNxSlgSwudUln6LLne86-H8jxKHuXDTQtqoHP8aa5gWVgjJefYxKwQhJYeYJvdqhJvgYAzT1OrhOhZeaknrTUz0Xs1_bnmYJ_rH3HXUH9ogeiknAxQ4I0RzV_0XXa9sk5vt7DH8Dugak1A</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Lee, P. T</creator><creator>Lowinsohn, D</creator><creator>Compton, R. G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20140101</creationdate><title>The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes</title><author>Lee, P. T ; Lowinsohn, D ; Compton, R. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-69d8f24bdcf7bd221be1c2d9304e15bfb737815509a913862656d12bfbed6db83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ascorbic acid</topic><topic>Ascorbic Acid - analysis</topic><topic>Calibration</topic><topic>Carbon</topic><topic>Carbon - chemistry</topic><topic>Cysteine</topic><topic>Cysteine - analysis</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrodes</topic><topic>Glassy carbon</topic><topic>Glutathione</topic><topic>Glutathione - analysis</topic><topic>Homocysteine - analysis</topic><topic>Limit of Detection</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, P. T</creatorcontrib><creatorcontrib>Lowinsohn, D</creatorcontrib><creatorcontrib>Compton, R. G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, P. T</au><au>Lowinsohn, D</au><au>Compton, R. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>139</volume><issue>15</issue><spage>3755</spage><epage>3762</epage><pages>3755-3762</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence and presence of glutathione, cysteine and ascorbic acid using cyclic voltammetry and square wave voltammetry. A calibration curve of homocysteine detection was determined and the sensitivity is (0.20 ± 0.02) μA μM
−1
and the limit of detection is 660 nM within the linear range. Lastly, commercially available multi walled carbon nanotube screen printed electrodes were applied to the system for selective homocysteine detection. This work presents a potential practical application towards medical applications as it can be highly beneficial towards quality healthcare management.
The detection of homocysteine, HCys, was achieved with the use of catechol
via
1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode.</abstract><cop>England</cop><pmid>24918102</pmid><doi>10.1039/c4an00372a</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analyst (London), 2014-01, Vol.139 (15), p.3755-3762 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_pubmed_primary_24918102 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Ascorbic acid Ascorbic Acid - analysis Calibration Carbon Carbon - chemistry Cysteine Cysteine - analysis Electrochemical Techniques - methods Electrodes Glassy carbon Glutathione Glutathione - analysis Homocysteine - analysis Limit of Detection Nanotubes, Carbon - chemistry Voltammetry |
| title | The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes |
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