Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia

In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies o...

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Veröffentlicht in:Mycologia 2014-03, Vol.106 (2), p.339-352
Hauptverfasser: Mc Cargo, Patricia D., Iannone, Leopoldo J., Vignale, María Victoria, Schardl, Christopher L., Rossi, María Susana
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container_issue 2
container_start_page 339
container_title Mycologia
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creator Mc Cargo, Patricia D.
Iannone, Leopoldo J.
Vignale, María Victoria
Schardl, Christopher L.
Rossi, María Susana
description In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.
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The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.</description><identifier>ISSN: 0027-5514</identifier><identifier>EISSN: 1557-2536</identifier><identifier>DOI: 10.3852/106.2.339</identifier><identifier>PMID: 24782501</identifier><language>eng</language><publisher>England: Taylor &amp; Francis</publisher><subject><![CDATA[Biodiversity ; Bromus ; Bromus setifolius ; DNA, Fungal - genetics ; DNA, Ribosomal Spacer - genetics ; endophyte diversity ; Epichloe ; Epichloe - classification ; Epichloe - genetics ; Epichloe - growth & development ; Epichloe - isolation & purification ; Epichloe typhina ; Epichloë ; Molecular Sequence Data ; Neotyphodium ; Neotyphodium - classification ; Neotyphodium - genetics ; Neotyphodium - growth & development ; Neotyphodium - isolation & purification ; Neotyphodium tembladerae ; Phleum alpinum ; Phylogeny ; Poaceae - microbiology ; Poaceae - physiology ; Spores, Fungal - classification ; Spores, Fungal - genetics ; Spores, Fungal - growth & development ; Spores, Fungal - isolation & purification ; Symbiosis ; SYSTEMATICS]]></subject><ispartof>Mycologia, 2014-03, Vol.106 (2), p.339-352</ispartof><rights>2014 by The Mycological Society of America 2014</rights><rights>2014 by The Mycological Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-91333825e16b118a877f23cbeba1ff4334c8faeff155e4e62b6ee726dd2e08673</citedby><cites>FETCH-LOGICAL-c385t-91333825e16b118a877f23cbeba1ff4334c8faeff155e4e62b6ee726dd2e08673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26506860$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26506860$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24782501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mc Cargo, Patricia D.</creatorcontrib><creatorcontrib>Iannone, Leopoldo J.</creatorcontrib><creatorcontrib>Vignale, María Victoria</creatorcontrib><creatorcontrib>Schardl, Christopher L.</creatorcontrib><creatorcontrib>Rossi, María Susana</creatorcontrib><title>Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia</title><title>Mycologia</title><addtitle>Mycologia</addtitle><description>In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. 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Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. 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development</subject><subject>Spores, Fungal - isolation &amp; purification</subject><subject>Symbiosis</subject><subject>SYSTEMATICS</subject><issn>0027-5514</issn><issn>1557-2536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1OGzEUhS3UioTAog_QysuymNQ_Y89kGSFakJBAApbI8niuE6OZcWp7GuWZeAxerK7Cz4JFV0dX57v3Hh2EvlAy57VgPyiRczbnfHGAplSIqmCCy09oSgirCiFoOUFHMT7mMbvkEE1YWdVMEDpFD7cbMA4ibt0fCNGlHfYWn2-cWXf--QnHXd84n5zBW5fWOG09XgUdY96wwfc4-jGtIQx4GVYwJDeAHvCNTnrlB6eP0WeruwgnLzpD9z_P784uiqvrX5dny6vC5PypWFDOeQ4EVDaU1rquKsu4aaDR1NqS89LUVoO1OT6UIFkjASom25YBqWXFZ-j7_u4m-N8jxKR6Fw10nR7Aj1HRSopFveBl-X9UMJI5mb_O0OkeNcHHGMCqTXC9DjtFifpXfFapmMrFZ_bby9mx6aF9I1-bzsDXPfAYkw_vvhRE1pJkv9z7brA-9HrrQ9eqpHedDzbowbio-Me_fwEwDJmo</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Mc Cargo, Patricia D.</creator><creator>Iannone, Leopoldo J.</creator><creator>Vignale, María Victoria</creator><creator>Schardl, Christopher L.</creator><creator>Rossi, María Susana</creator><general>Taylor &amp; 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Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.</abstract><cop>England</cop><pub>Taylor &amp; Francis</pub><pmid>24782501</pmid><doi>10.3852/106.2.339</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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ispartof Mycologia, 2014-03, Vol.106 (2), p.339-352
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language eng
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source MEDLINE; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection
subjects Biodiversity
Bromus
Bromus setifolius
DNA, Fungal - genetics
DNA, Ribosomal Spacer - genetics
endophyte diversity
Epichloe
Epichloe - classification
Epichloe - genetics
Epichloe - growth & development
Epichloe - isolation & purification
Epichloe typhina
Epichloë
Molecular Sequence Data
Neotyphodium
Neotyphodium - classification
Neotyphodium - genetics
Neotyphodium - growth & development
Neotyphodium - isolation & purification
Neotyphodium tembladerae
Phleum alpinum
Phylogeny
Poaceae - microbiology
Poaceae - physiology
Spores, Fungal - classification
Spores, Fungal - genetics
Spores, Fungal - growth & development
Spores, Fungal - isolation & purification
Symbiosis
SYSTEMATICS
title Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
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