Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies o...
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Veröffentlicht in: | Mycologia 2014-03, Vol.106 (2), p.339-352 |
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description | In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species. |
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The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.</description><identifier>ISSN: 0027-5514</identifier><identifier>EISSN: 1557-2536</identifier><identifier>DOI: 10.3852/106.2.339</identifier><identifier>PMID: 24782501</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject><![CDATA[Biodiversity ; Bromus ; Bromus setifolius ; DNA, Fungal - genetics ; DNA, Ribosomal Spacer - genetics ; endophyte diversity ; Epichloe ; Epichloe - classification ; Epichloe - genetics ; Epichloe - growth & development ; Epichloe - isolation & purification ; Epichloe typhina ; Epichloë ; Molecular Sequence Data ; Neotyphodium ; Neotyphodium - classification ; Neotyphodium - genetics ; Neotyphodium - growth & development ; Neotyphodium - isolation & purification ; Neotyphodium tembladerae ; Phleum alpinum ; Phylogeny ; Poaceae - microbiology ; Poaceae - physiology ; Spores, Fungal - classification ; Spores, Fungal - genetics ; Spores, Fungal - growth & development ; Spores, Fungal - isolation & purification ; Symbiosis ; SYSTEMATICS]]></subject><ispartof>Mycologia, 2014-03, Vol.106 (2), p.339-352</ispartof><rights>2014 by The Mycological Society of America 2014</rights><rights>2014 by The Mycological Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-91333825e16b118a877f23cbeba1ff4334c8faeff155e4e62b6ee726dd2e08673</citedby><cites>FETCH-LOGICAL-c385t-91333825e16b118a877f23cbeba1ff4334c8faeff155e4e62b6ee726dd2e08673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26506860$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26506860$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24782501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mc Cargo, Patricia D.</creatorcontrib><creatorcontrib>Iannone, Leopoldo J.</creatorcontrib><creatorcontrib>Vignale, María Victoria</creatorcontrib><creatorcontrib>Schardl, Christopher L.</creatorcontrib><creatorcontrib>Rossi, María Susana</creatorcontrib><title>Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia</title><title>Mycologia</title><addtitle>Mycologia</addtitle><description>In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.</description><subject>Biodiversity</subject><subject>Bromus</subject><subject>Bromus setifolius</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>endophyte diversity</subject><subject>Epichloe</subject><subject>Epichloe - classification</subject><subject>Epichloe - genetics</subject><subject>Epichloe - growth & development</subject><subject>Epichloe - isolation & purification</subject><subject>Epichloe typhina</subject><subject>Epichloë</subject><subject>Molecular Sequence Data</subject><subject>Neotyphodium</subject><subject>Neotyphodium - classification</subject><subject>Neotyphodium - genetics</subject><subject>Neotyphodium - growth & development</subject><subject>Neotyphodium - isolation & purification</subject><subject>Neotyphodium tembladerae</subject><subject>Phleum alpinum</subject><subject>Phylogeny</subject><subject>Poaceae - microbiology</subject><subject>Poaceae - physiology</subject><subject>Spores, Fungal - classification</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - growth & development</subject><subject>Spores, Fungal - isolation & purification</subject><subject>Symbiosis</subject><subject>SYSTEMATICS</subject><issn>0027-5514</issn><issn>1557-2536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1OGzEUhS3UioTAog_QysuymNQ_Y89kGSFakJBAApbI8niuE6OZcWp7GuWZeAxerK7Cz4JFV0dX57v3Hh2EvlAy57VgPyiRczbnfHGAplSIqmCCy09oSgirCiFoOUFHMT7mMbvkEE1YWdVMEDpFD7cbMA4ibt0fCNGlHfYWn2-cWXf--QnHXd84n5zBW5fWOG09XgUdY96wwfc4-jGtIQx4GVYwJDeAHvCNTnrlB6eP0WeruwgnLzpD9z_P784uiqvrX5dny6vC5PypWFDOeQ4EVDaU1rquKsu4aaDR1NqS89LUVoO1OT6UIFkjASom25YBqWXFZ-j7_u4m-N8jxKR6Fw10nR7Aj1HRSopFveBl-X9UMJI5mb_O0OkeNcHHGMCqTXC9DjtFifpXfFapmMrFZ_bby9mx6aF9I1-bzsDXPfAYkw_vvhRE1pJkv9z7brA-9HrrQ9eqpHedDzbowbio-Me_fwEwDJmo</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Mc Cargo, Patricia D.</creator><creator>Iannone, Leopoldo J.</creator><creator>Vignale, María Victoria</creator><creator>Schardl, Christopher L.</creator><creator>Rossi, María Susana</creator><general>Taylor & Francis</general><general>Mycological Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SN</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20140301</creationdate><title>Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia</title><author>Mc Cargo, Patricia D. ; Iannone, Leopoldo J. ; Vignale, María Victoria ; Schardl, Christopher L. ; Rossi, María Susana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-91333825e16b118a877f23cbeba1ff4334c8faeff155e4e62b6ee726dd2e08673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biodiversity</topic><topic>Bromus</topic><topic>Bromus setifolius</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>endophyte diversity</topic><topic>Epichloe</topic><topic>Epichloe - classification</topic><topic>Epichloe - genetics</topic><topic>Epichloe - growth & development</topic><topic>Epichloe - isolation & purification</topic><topic>Epichloe typhina</topic><topic>Epichloë</topic><topic>Molecular Sequence Data</topic><topic>Neotyphodium</topic><topic>Neotyphodium - classification</topic><topic>Neotyphodium - genetics</topic><topic>Neotyphodium - growth & development</topic><topic>Neotyphodium - isolation & purification</topic><topic>Neotyphodium tembladerae</topic><topic>Phleum alpinum</topic><topic>Phylogeny</topic><topic>Poaceae - microbiology</topic><topic>Poaceae - physiology</topic><topic>Spores, Fungal - classification</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - growth & development</topic><topic>Spores, Fungal - isolation & purification</topic><topic>Symbiosis</topic><topic>SYSTEMATICS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mc Cargo, Patricia D.</creatorcontrib><creatorcontrib>Iannone, Leopoldo J.</creatorcontrib><creatorcontrib>Vignale, María Victoria</creatorcontrib><creatorcontrib>Schardl, Christopher L.</creatorcontrib><creatorcontrib>Rossi, María Susana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Mycologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mc Cargo, Patricia D.</au><au>Iannone, Leopoldo J.</au><au>Vignale, María Victoria</au><au>Schardl, Christopher L.</au><au>Rossi, María Susana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia</atitle><jtitle>Mycologia</jtitle><addtitle>Mycologia</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>106</volume><issue>2</issue><spage>339</spage><epage>352</epage><pages>339-352</pages><issn>0027-5514</issn><eissn>1557-2536</eissn><abstract>In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translation-elongation factor 1-α [tefA] and β-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>24782501</pmid><doi>10.3852/106.2.339</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Biodiversity Bromus Bromus setifolius DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics endophyte diversity Epichloe Epichloe - classification Epichloe - genetics Epichloe - growth & development Epichloe - isolation & purification Epichloe typhina Epichloë Molecular Sequence Data Neotyphodium Neotyphodium - classification Neotyphodium - genetics Neotyphodium - growth & development Neotyphodium - isolation & purification Neotyphodium tembladerae Phleum alpinum Phylogeny Poaceae - microbiology Poaceae - physiology Spores, Fungal - classification Spores, Fungal - genetics Spores, Fungal - growth & development Spores, Fungal - isolation & purification Symbiosis SYSTEMATICS |
title | Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia |
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