Isolation and DNA Sequence of a cDNA Clone Encoding a Lymphocyte Adhesion Receptor for High Endothelium
The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by highwalled endothelial cells. Lymphocyte adhesion to and extravasation at these sites is...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-06, Vol.86 (12), p.4659-4663 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Idzerda, Rejean L. Carter, William G. Nottenburg, Carol Wayner, Elizabeth A. Gallatin, W. Michael St. John, Tom |
| description | The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by highwalled endothelial cells. Lymphocyte adhesion to and extravasation at these sites is associated with the expression of specific lymphoid receptors for this specialized venule endothelium. We report here the molecular cloning from a baboon lymphoid cell line of a cDNA that encodes an adhesion receptor for HEV. The 362-amino acid protein encoded by this cDNA is not present in any of the data bases examined. The mature protein, resulting from the cleavage of a putative 20-amino acid signal peptide, has a calculated molecular mass of only 37 kDa, indicating that the 90-kDa cell surface protein is highly modified. The 342 amino acids, which lack any repeated sequences of significant length, encompass an extracellular domain (250 amino acids), a putative transmembrane domain (20 amino acids), and a cytoplasmic domain (72 amino acids). |
| doi_str_mv | 10.1073/pnas.86.12.4659 |
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Michael ; St. John, Tom</creator><creatorcontrib>Idzerda, Rejean L. ; Carter, William G. ; Nottenburg, Carol ; Wayner, Elizabeth A. ; Gallatin, W. Michael ; St. John, Tom</creatorcontrib><description>The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by highwalled endothelial cells. Lymphocyte adhesion to and extravasation at these sites is associated with the expression of specific lymphoid receptors for this specialized venule endothelium. We report here the molecular cloning from a baboon lymphoid cell line of a cDNA that encodes an adhesion receptor for HEV. The 362-amino acid protein encoded by this cDNA is not present in any of the data bases examined. The mature protein, resulting from the cleavage of a putative 20-amino acid signal peptide, has a calculated molecular mass of only 37 kDa, indicating that the 90-kDa cell surface protein is highly modified. The 342 amino acids, which lack any repeated sequences of significant length, encompass an extracellular domain (250 amino acids), a putative transmembrane domain (20 amino acids), and a cytoplasmic domain (72 amino acids).</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.12.4659</identifier><identifier>PMID: 2471974</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Antibodies ; Base Sequence ; Biological and medical sciences ; Cell adhesion ; Cell Line ; Cloning, Molecular ; Complementary DNA ; DNA - genetics ; DNA - isolation & purification ; Endothelium, Vascular - physiology ; Epitopes ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; genes ; Hepatitis E virus ; Immunobiology ; Lymphocytes ; Lymphocytes - physiology ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Molecular Sequence Data ; Molecules ; Papio ; Protein Conformation ; Proteins ; Receptors ; Receptors, Immunologic - genetics ; Receptors, Immunologic - immunology ; Receptors, Lymphocyte Homing</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-06, Vol.86 (12), p.4659-4663</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-4aff7bd21a6e9b8405f993635f57a7686954f502b90d0c35ea172eb66e558a723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/33751$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/33751$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7320797$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2471974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Idzerda, Rejean L.</creatorcontrib><creatorcontrib>Carter, William G.</creatorcontrib><creatorcontrib>Nottenburg, Carol</creatorcontrib><creatorcontrib>Wayner, Elizabeth A.</creatorcontrib><creatorcontrib>Gallatin, W. Michael</creatorcontrib><creatorcontrib>St. John, Tom</creatorcontrib><title>Isolation and DNA Sequence of a cDNA Clone Encoding a Lymphocyte Adhesion Receptor for High Endothelium</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by highwalled endothelial cells. Lymphocyte adhesion to and extravasation at these sites is associated with the expression of specific lymphoid receptors for this specialized venule endothelium. We report here the molecular cloning from a baboon lymphoid cell line of a cDNA that encodes an adhesion receptor for HEV. The 362-amino acid protein encoded by this cDNA is not present in any of the data bases examined. The mature protein, resulting from the cleavage of a putative 20-amino acid signal peptide, has a calculated molecular mass of only 37 kDa, indicating that the 90-kDa cell surface protein is highly modified. The 342 amino acids, which lack any repeated sequences of significant length, encompass an extracellular domain (250 amino acids), a putative transmembrane domain (20 amino acids), and a cytoplasmic domain (72 amino acids).</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell adhesion</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Endothelium, Vascular - physiology</subject><subject>Epitopes</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>genes</subject><subject>Hepatitis E virus</subject><subject>Immunobiology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - physiology</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Papio</subject><subject>Protein Conformation</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Immunologic - immunology</subject><subject>Receptors, Lymphocyte Homing</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2L1DAYxoMo67h6FgSlB9FTZ_Od5uBhGFd3YVDw4xzSNJl2ySS1acX5702ZOrgXPYTA-_ye932TB4DnCK4RFOSqDzqtK75GeE05kw_ACkGJSk4lfAhWEGJRVhTTx-BJSncQQskqeAEuMBVICroC-9sUvR67GAodmuL9p03x1f6YbDC2iK7QhZlLWx-DLa6DiU0X9rm6Ox76NprjaItN09o0-79YY_sxDoXL56bbt9nQxLG1vpsOT8Ejp32yz5b7Enz_cP1te1PuPn-83W52pWGVGEuqnRN1g5HmVtYVhcxJSThhjgkteMUlo45BXEvYQEOY1UhgW3NuGau0wOQSvDv17af6YBtjwzhor_qhO-jhqKLu1H0ldK3ax58KV4IQmP1vFv8Q8zekUR26ZKz3Otg4JSUkxBXJG_0PRAwzzijJ4NUJNENMabDuvAyCas5QzRmqiiuE1Zxhdrz8-w1nfgkt668XXSejvRt0MF06Y4JgKKTI2KsFm_v_Ue_NeftPQLnJ-9H-GjP54kTepRzwGSVEMER-A9iVxZk</recordid><startdate>19890601</startdate><enddate>19890601</enddate><creator>Idzerda, Rejean L.</creator><creator>Carter, William G.</creator><creator>Nottenburg, Carol</creator><creator>Wayner, Elizabeth A.</creator><creator>Gallatin, W. Michael</creator><creator>St. John, Tom</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890601</creationdate><title>Isolation and DNA Sequence of a cDNA Clone Encoding a Lymphocyte Adhesion Receptor for High Endothelium</title><author>Idzerda, Rejean L. ; Carter, William G. ; Nottenburg, Carol ; Wayner, Elizabeth A. ; Gallatin, W. Michael ; St. John, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-4aff7bd21a6e9b8405f993635f57a7686954f502b90d0c35ea172eb66e558a723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell adhesion</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Endothelium, Vascular - physiology</topic><topic>Epitopes</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>genes</topic><topic>Hepatitis E virus</topic><topic>Immunobiology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - physiology</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Papio</topic><topic>Protein Conformation</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Immunologic - genetics</topic><topic>Receptors, Immunologic - immunology</topic><topic>Receptors, Lymphocyte Homing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Idzerda, Rejean L.</creatorcontrib><creatorcontrib>Carter, William G.</creatorcontrib><creatorcontrib>Nottenburg, Carol</creatorcontrib><creatorcontrib>Wayner, Elizabeth A.</creatorcontrib><creatorcontrib>Gallatin, W. 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We report here the molecular cloning from a baboon lymphoid cell line of a cDNA that encodes an adhesion receptor for HEV. The 362-amino acid protein encoded by this cDNA is not present in any of the data bases examined. The mature protein, resulting from the cleavage of a putative 20-amino acid signal peptide, has a calculated molecular mass of only 37 kDa, indicating that the 90-kDa cell surface protein is highly modified. The 342 amino acids, which lack any repeated sequences of significant length, encompass an extracellular domain (250 amino acids), a putative transmembrane domain (20 amino acids), and a cytoplasmic domain (72 amino acids).</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2471974</pmid><doi>10.1073/pnas.86.12.4659</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Animals Antibodies Base Sequence Biological and medical sciences Cell adhesion Cell Line Cloning, Molecular Complementary DNA DNA - genetics DNA - isolation & purification Endothelium, Vascular - physiology Epitopes Epitopes - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology genes Hepatitis E virus Immunobiology Lymphocytes Lymphocytes - physiology Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Molecular Sequence Data Molecules Papio Protein Conformation Proteins Receptors Receptors, Immunologic - genetics Receptors, Immunologic - immunology Receptors, Lymphocyte Homing |
| title | Isolation and DNA Sequence of a cDNA Clone Encoding a Lymphocyte Adhesion Receptor for High Endothelium |
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