Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens
We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is de...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (4), p.1362-1366 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1366 |
|---|---|
| container_issue | 4 |
| container_start_page | 1362 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 86 |
| creator | Keydar, I. Chou, C. S. Hareuveni, M. Tsarfaty, I. Sahar, E. Selzer, G. Chaitchik, S. Hizi, A. |
| description | We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is detected in the growth medium of T47D cells as well as in pleural effusion fluids from breast adenocarcinoma patients. No detectable levels of this antigen could be observed in pleural effusions of patients with cancers other than of breast origin. The H23-related antigen was localized in the cytoplasm of breast tumor cells as well as on the cell surface of both T47D cells and metastatic cells from breast cancer patients. A survey of tissue from 812 patients was performed by using H23 in an indirect immunoperoxidase assay. The results showed that the antigen was detectable in 91% of all breast tumors tested. No cytoplasmic staining was observed in either normal tissues or nonbreast carcinomas. Only one of the benign breast tissues tested (out of a total of 56 samples of tissue) was positive for this antigen. Given the ability of this antibody to specifically detect breast tumor cells, H23 may be of importance in diagnosis and in clinical follow-up of patients for the detection of metastatic lesions by imaging and for therapy. |
| doi_str_mv | 10.1073/pnas.86.4.1362 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmed_primary_2465551</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>33411</jstor_id><sourcerecordid>33411</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-c1335ced526e5a8638843b01018e634e8534895fe84247eccae9c541dbff583c3</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS1EVZbClQMSUg5Vbwl2_BHnwGFZFVqpCA7lbHmdydaV115sB7X89STdJVokJE6W_X4z88YPoTcEVwQ39P3O61RJUbGKUFE_QwuCW1IK1uLnaIFx3ZSS1ewFepnSPca45RKfotOaCc45WSD4FkM3mGyDL7TvitWdjtpkiPaXfnoMffEl-GBc8NoVS5_tOnQWUnHdwXjpH63fFB8j6JSL22EbYrlMKRirM3RP-AZ8eoVOeu0SvD6cZ-j7p8vb1VV58_Xz9Wp5UxpORC4NoZQb6HgtgGspqJSMrjHBRIKgDCSnTLa8h2mlBozR0BrOSLfuey6poWfow77vblhvoTOjw6id2kW71fFRBW3V34q3d2oTfqpaCtHisf7iUB_DjwFSVlubDDinPYQhqUZK3orx2_8HEk7ktMwIVnvQxJBShH42Q7CaAlRTgEoKxdQU4Fjw7niFGT8kNurnB10no10ftTc2zVhDxh68PjI4tf-jzmNUPziX4SEfzfsnOOpv9_p9yiHOAKWMEPobm0jGbA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15181335</pqid></control><display><type>article</type><title>Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Keydar, I. ; Chou, C. S. ; Hareuveni, M. ; Tsarfaty, I. ; Sahar, E. ; Selzer, G. ; Chaitchik, S. ; Hizi, A.</creator><creatorcontrib>Keydar, I. ; Chou, C. S. ; Hareuveni, M. ; Tsarfaty, I. ; Sahar, E. ; Selzer, G. ; Chaitchik, S. ; Hizi, A.</creatorcontrib><description>We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is detected in the growth medium of T47D cells as well as in pleural effusion fluids from breast adenocarcinoma patients. No detectable levels of this antigen could be observed in pleural effusions of patients with cancers other than of breast origin. The H23-related antigen was localized in the cytoplasm of breast tumor cells as well as on the cell surface of both T47D cells and metastatic cells from breast cancer patients. A survey of tissue from 812 patients was performed by using H23 in an indirect immunoperoxidase assay. The results showed that the antigen was detectable in 91% of all breast tumors tested. No cytoplasmic staining was observed in either normal tissues or nonbreast carcinomas. Only one of the benign breast tissues tested (out of a total of 56 samples of tissue) was positive for this antigen. Given the ability of this antibody to specifically detect breast tumor cells, H23 may be of importance in diagnosis and in clinical follow-up of patients for the detection of metastatic lesions by imaging and for therapy.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.4.1362</identifier><identifier>PMID: 2465551</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Adenocarcinoma ; Animals ; Antibodies ; Antibodies, immunoglobulins ; Antibodies, Monoclonal ; Antigens ; Antigens, Surface - analysis ; Antigens, Viral, Tumor - analysis ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Breast cancer ; Breast ductal carcinoma ; Breast neoplasms ; Breast Neoplasms - analysis ; Breast Neoplasms - pathology ; Breasts ; Cell Line ; Enzyme-Linked Immunosorbent Assay ; Epitopes - analysis ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins ; Humans ; Immunoenzyme Techniques ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; Molecular Weight ; Monoclonal antibodies ; Neoplasms - analysis ; Reference Values ; Retroviridae - isolation & purification ; Thyroid cancer ; Tumor cell line ; Tumors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-02, Vol.86 (4), p.1362-1366</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-c1335ced526e5a8638843b01018e634e8534895fe84247eccae9c541dbff583c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/33411$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/33411$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27933,27934,53800,53802,58026,58259</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7162352$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2465551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keydar, I.</creatorcontrib><creatorcontrib>Chou, C. S.</creatorcontrib><creatorcontrib>Hareuveni, M.</creatorcontrib><creatorcontrib>Tsarfaty, I.</creatorcontrib><creatorcontrib>Sahar, E.</creatorcontrib><creatorcontrib>Selzer, G.</creatorcontrib><creatorcontrib>Chaitchik, S.</creatorcontrib><creatorcontrib>Hizi, A.</creatorcontrib><title>Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is detected in the growth medium of T47D cells as well as in pleural effusion fluids from breast adenocarcinoma patients. No detectable levels of this antigen could be observed in pleural effusions of patients with cancers other than of breast origin. The H23-related antigen was localized in the cytoplasm of breast tumor cells as well as on the cell surface of both T47D cells and metastatic cells from breast cancer patients. A survey of tissue from 812 patients was performed by using H23 in an indirect immunoperoxidase assay. The results showed that the antigen was detectable in 91% of all breast tumors tested. No cytoplasmic staining was observed in either normal tissues or nonbreast carcinomas. Only one of the benign breast tissues tested (out of a total of 56 samples of tissue) was positive for this antigen. Given the ability of this antibody to specifically detect breast tumor cells, H23 may be of importance in diagnosis and in clinical follow-up of patients for the detection of metastatic lesions by imaging and for therapy.</description><subject>Adenocarcinoma</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens</subject><subject>Antigens, Surface - analysis</subject><subject>Antigens, Viral, Tumor - analysis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Breast cancer</subject><subject>Breast ductal carcinoma</subject><subject>Breast neoplasms</subject><subject>Breast Neoplasms - analysis</subject><subject>Breast Neoplasms - pathology</subject><subject>Breasts</subject><subject>Cell Line</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - analysis</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>Monoclonal antibodies</subject><subject>Neoplasms - analysis</subject><subject>Reference Values</subject><subject>Retroviridae - isolation & purification</subject><subject>Thyroid cancer</subject><subject>Tumor cell line</subject><subject>Tumors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1EVZbClQMSUg5Vbwl2_BHnwGFZFVqpCA7lbHmdydaV115sB7X89STdJVokJE6W_X4z88YPoTcEVwQ39P3O61RJUbGKUFE_QwuCW1IK1uLnaIFx3ZSS1ewFepnSPca45RKfotOaCc45WSD4FkM3mGyDL7TvitWdjtpkiPaXfnoMffEl-GBc8NoVS5_tOnQWUnHdwXjpH63fFB8j6JSL22EbYrlMKRirM3RP-AZ8eoVOeu0SvD6cZ-j7p8vb1VV58_Xz9Wp5UxpORC4NoZQb6HgtgGspqJSMrjHBRIKgDCSnTLa8h2mlBozR0BrOSLfuey6poWfow77vblhvoTOjw6id2kW71fFRBW3V34q3d2oTfqpaCtHisf7iUB_DjwFSVlubDDinPYQhqUZK3orx2_8HEk7ktMwIVnvQxJBShH42Q7CaAlRTgEoKxdQU4Fjw7niFGT8kNurnB10no10ftTc2zVhDxh68PjI4tf-jzmNUPziX4SEfzfsnOOpv9_p9yiHOAKWMEPobm0jGbA</recordid><startdate>19890201</startdate><enddate>19890201</enddate><creator>Keydar, I.</creator><creator>Chou, C. S.</creator><creator>Hareuveni, M.</creator><creator>Tsarfaty, I.</creator><creator>Sahar, E.</creator><creator>Selzer, G.</creator><creator>Chaitchik, S.</creator><creator>Hizi, A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890201</creationdate><title>Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens</title><author>Keydar, I. ; Chou, C. S. ; Hareuveni, M. ; Tsarfaty, I. ; Sahar, E. ; Selzer, G. ; Chaitchik, S. ; Hizi, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-c1335ced526e5a8638843b01018e634e8534895fe84247eccae9c541dbff583c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adenocarcinoma</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens</topic><topic>Antigens, Surface - analysis</topic><topic>Antigens, Viral, Tumor - analysis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Breast cancer</topic><topic>Breast ductal carcinoma</topic><topic>Breast neoplasms</topic><topic>Breast Neoplasms - analysis</topic><topic>Breast Neoplasms - pathology</topic><topic>Breasts</topic><topic>Cell Line</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - analysis</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoproteins</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>Monoclonal antibodies</topic><topic>Neoplasms - analysis</topic><topic>Reference Values</topic><topic>Retroviridae - isolation & purification</topic><topic>Thyroid cancer</topic><topic>Tumor cell line</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keydar, I.</creatorcontrib><creatorcontrib>Chou, C. S.</creatorcontrib><creatorcontrib>Hareuveni, M.</creatorcontrib><creatorcontrib>Tsarfaty, I.</creatorcontrib><creatorcontrib>Sahar, E.</creatorcontrib><creatorcontrib>Selzer, G.</creatorcontrib><creatorcontrib>Chaitchik, S.</creatorcontrib><creatorcontrib>Hizi, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keydar, I.</au><au>Chou, C. S.</au><au>Hareuveni, M.</au><au>Tsarfaty, I.</au><au>Sahar, E.</au><au>Selzer, G.</au><au>Chaitchik, S.</au><au>Hizi, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-02-01</date><risdate>1989</risdate><volume>86</volume><issue>4</issue><spage>1362</spage><epage>1366</epage><pages>1362-1366</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is detected in the growth medium of T47D cells as well as in pleural effusion fluids from breast adenocarcinoma patients. No detectable levels of this antigen could be observed in pleural effusions of patients with cancers other than of breast origin. The H23-related antigen was localized in the cytoplasm of breast tumor cells as well as on the cell surface of both T47D cells and metastatic cells from breast cancer patients. A survey of tissue from 812 patients was performed by using H23 in an indirect immunoperoxidase assay. The results showed that the antigen was detectable in 91% of all breast tumors tested. No cytoplasmic staining was observed in either normal tissues or nonbreast carcinomas. Only one of the benign breast tissues tested (out of a total of 56 samples of tissue) was positive for this antigen. Given the ability of this antibody to specifically detect breast tumor cells, H23 may be of importance in diagnosis and in clinical follow-up of patients for the detection of metastatic lesions by imaging and for therapy.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2465551</pmid><doi>10.1073/pnas.86.4.1362</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1989-02, Vol.86 (4), p.1362-1366 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_2465551 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenocarcinoma Animals Antibodies Antibodies, immunoglobulins Antibodies, Monoclonal Antigens Antigens, Surface - analysis Antigens, Viral, Tumor - analysis Biological and medical sciences Biomarkers, Tumor - analysis Breast cancer Breast ductal carcinoma Breast neoplasms Breast Neoplasms - analysis Breast Neoplasms - pathology Breasts Cell Line Enzyme-Linked Immunosorbent Assay Epitopes - analysis Female Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins Humans Immunoenzyme Techniques Mice Mice, Inbred BALB C Molecular immunology Molecular Weight Monoclonal antibodies Neoplasms - analysis Reference Values Retroviridae - isolation & purification Thyroid cancer Tumor cell line Tumors |
| title | Production and Characterization of Monoclonal Antibodies Identifying Breast Tumor-Associated Antigens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T05%3A44%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20and%20Characterization%20of%20Monoclonal%20Antibodies%20Identifying%20Breast%20Tumor-Associated%20Antigens&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Keydar,%20I.&rft.date=1989-02-01&rft.volume=86&rft.issue=4&rft.spage=1362&rft.epage=1366&rft.pages=1362-1366&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.86.4.1362&rft_dat=%3Cjstor_pubme%3E33411%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15181335&rft_id=info:pmid/2465551&rft_jstor_id=33411&rfr_iscdi=true |