Addition of a Foreign Oligopeptide to the Major Capsid Protein of Poliovirus

Insertion mutants of type 3 poliovirus (Sabin strain) were constructed that encode additional amino acid sequences at the level of residue 100 of the capsid polypeptide VP1 within the neutralization site 1, corresponding to a loop on the capsid surface. The addition of a tri- or hexapeptide did not...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1988-11, Vol.85 (22), p.8668-8672
Hauptverfasser: Colbère-Garapin, Florence, Christodoulou, Christina, Crainic, Radu, Garapin, Axel-Claude, Candrea, Adina
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container_end_page 8672
container_issue 22
container_start_page 8668
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 85
creator Colbère-Garapin, Florence
Christodoulou, Christina
Crainic, Radu
Garapin, Axel-Claude
Candrea, Adina
description Insertion mutants of type 3 poliovirus (Sabin strain) were constructed that encode additional amino acid sequences at the level of residue 100 of the capsid polypeptide VP1 within the neutralization site 1, corresponding to a loop on the capsid surface. The addition of a tri- or hexapeptide did not hamper virus viability. The antigenic pattern of insertion mutants was only modified locally: all mutants lost reactivity of neutralization site 1 with the corresponding monoclonal antibodies, while the reactivity of sites 2 and 3 was unaffected by the insertion. We have shown for one of the mutants--vFG68--that the antigenic specificity of the neutralization site 1 was replaced by a new one. Although vFG68 differs from its parental Sabin strain only by the addition of three amino acids within VP1, neutralizing antibodies specific for vFG68 were induced by the native virion as well as by the heat-denatured mutated virions. Our results demonstrate that an oligopeptide of three or six amino acids can lengthen VP1 at the level of antigenic site 1 without affecting virus multiplication and that this foreign peptide is exposed on the virion surface.
doi_str_mv 10.1073/pnas.85.22.8668
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Psychology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Mutation</subject><subject>Oligopeptides</subject><subject>Plasmids</subject><subject>Poliovirus</subject><subject>Poliovirus - genetics</subject><subject>RNA</subject><subject>Vero cells</subject><subject>Virions</subject><subject>Virology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9rFDEUB_AgSl1bz4Kg5FD0NNv8mvw4lqW1wpb2oOeQnXlTs8xOxiRT9L9v1h0XvNhLAnmf9_L4IvSOkiUlil-Mg0tLXS8ZW2op9Qu0oMTQSgpDXqIFIUxVWjDxGr1JaUsIMbUmJ-iECUm0qhdofdm2Pvsw4NBhh69DBP8w4LveP4QRxuxbwDng_APwrduGiFduTL7F9zFk8H-67kPvw6OPUzpDrzrXJ3g736fo-_XVt9VNtb778nV1ua4aYWSulGo7RSV3nRBagzOamvKmDSOUs9bQjVNMCS1b6hoOeiM2XSOEA61rYBz4Kfp0mDvG8HOClO3Opwb63g0QpmSVrqkox7OQ1kTSkkOBFwfYxJBShM6O0e9c_G0psfug7T5oq2vLmN0HXTo-zKOnzQ7ao5-TLfXzue5S4_ouuqHx6cgUq4Xh5jlWPpLGkMI-zmy_xt_qP-t8_i-w3dT3GX7lIt8f5DblEI-UM00EfwKRkLJ9</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>Colbère-Garapin, Florence</creator><creator>Christodoulou, Christina</creator><creator>Crainic, Radu</creator><creator>Garapin, Axel-Claude</creator><creator>Candrea, Adina</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19881101</creationdate><title>Addition of a Foreign Oligopeptide to the Major Capsid Protein of Poliovirus</title><author>Colbère-Garapin, Florence ; Christodoulou, Christina ; Crainic, Radu ; Garapin, Axel-Claude ; Candrea, Adina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-77df7163af4488ea981977d8920132d91ba727486d1ac3e8b4bfc44ae885e23e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Capsid</topic><topic>Capsid - genetics</topic><topic>Capsid - immunology</topic><topic>Capsid proteins</topic><topic>Codon</topic><topic>DNA Transposable Elements</topic><topic>Epitopes</topic><topic>Epitopes - analysis</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Mutation</topic><topic>Oligopeptides</topic><topic>Plasmids</topic><topic>Poliovirus</topic><topic>Poliovirus - genetics</topic><topic>RNA</topic><topic>Vero cells</topic><topic>Virions</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colbère-Garapin, Florence</creatorcontrib><creatorcontrib>Christodoulou, Christina</creatorcontrib><creatorcontrib>Crainic, Radu</creatorcontrib><creatorcontrib>Garapin, Axel-Claude</creatorcontrib><creatorcontrib>Candrea, Adina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colbère-Garapin, Florence</au><au>Christodoulou, Christina</au><au>Crainic, Radu</au><au>Garapin, Axel-Claude</au><au>Candrea, Adina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Addition of a Foreign Oligopeptide to the Major Capsid Protein of Poliovirus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>85</volume><issue>22</issue><spage>8668</spage><epage>8672</epage><pages>8668-8672</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Insertion mutants of type 3 poliovirus (Sabin strain) were constructed that encode additional amino acid sequences at the level of residue 100 of the capsid polypeptide VP1 within the neutralization site 1, corresponding to a loop on the capsid surface. The addition of a tri- or hexapeptide did not hamper virus viability. The antigenic pattern of insertion mutants was only modified locally: all mutants lost reactivity of neutralization site 1 with the corresponding monoclonal antibodies, while the reactivity of sites 2 and 3 was unaffected by the insertion. We have shown for one of the mutants--vFG68--that the antigenic specificity of the neutralization site 1 was replaced by a new one. Although vFG68 differs from its parental Sabin strain only by the addition of three amino acids within VP1, neutralizing antibodies specific for vFG68 were induced by the native virion as well as by the heat-denatured mutated virions. 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subjects Amino Acid Sequence
Amino acids
Base Sequence
Biological and medical sciences
Capsid
Capsid - genetics
Capsid - immunology
Capsid proteins
Codon
DNA Transposable Elements
Epitopes
Epitopes - analysis
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
Mutation
Oligopeptides
Plasmids
Poliovirus
Poliovirus - genetics
RNA
Vero cells
Virions
Virology
Viruses
title Addition of a Foreign Oligopeptide to the Major Capsid Protein of Poliovirus
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