Cell Shape Controls Terminal Differentiation of Human Epidermal Keratinocytes
Cultures of human epidermal keratinocytes provide a useful experimental model with which to study the factors that regulate cell proliferation and terminal differentiation. One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprive...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (15), p.5576-5580 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Watt, Fiona M. Jordan, Peter W. O'Neill, Charles H. |
| description | Cultures of human epidermal keratinocytes provide a useful experimental model with which to study the factors that regulate cell proliferation and terminal differentiation. One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprived of substratum and intercellular contact. We have now investigated whether area of substratum contact, and hence cell shape, can regulate terminal differentiation. Keratinocytes were grown on circular adhesive islands that prevented cell-cell contact. By varying island area we could vary cell shape from fully spread to almost spherical. We found that when substratum contact was restricted, DNA synthesis was inhibited and expression of involucrin, a marker of terminal differentiation, was stimulated. Inhibition of proliferation was not a sufficient stimulus for involucrin synthesis in fully spread cells. When DNA synthesis and involucrin expression were plotted against contact area, classic dose-response curves were obtained. Thus cell shape acts as a signal for the terminal differentiation of keratinocytes in culture. |
| doi_str_mv | 10.1073/pnas.85.15.5576 |
| format | Article |
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One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprived of substratum and intercellular contact. We have now investigated whether area of substratum contact, and hence cell shape, can regulate terminal differentiation. Keratinocytes were grown on circular adhesive islands that prevented cell-cell contact. By varying island area we could vary cell shape from fully spread to almost spherical. We found that when substratum contact was restricted, DNA synthesis was inhibited and expression of involucrin, a marker of terminal differentiation, was stimulated. Inhibition of proliferation was not a sufficient stimulus for involucrin synthesis in fully spread cells. When DNA synthesis and involucrin expression were plotted against contact area, classic dose-response curves were obtained. Thus cell shape acts as a signal for the terminal differentiation of keratinocytes in culture.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.85.15.5576</identifier><identifier>PMID: 2456572</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>B lymphocytes ; Cell Adhesion ; Cell Communication ; Cell culture techniques ; Cell Differentiation ; Cell Division ; Cells ; Cells, Cultured ; Cellular differentiation ; Cultured cells ; DNA ; DNA - biosynthesis ; Electrophoresis, Polyacrylamide Gel ; Epidermal Cells ; Epidermis - metabolism ; Epidermis - ultrastructure ; Fluorescent Antibody Technique ; foreskin ; Humans ; Keratinocytes ; Keratins - physiology ; Microscopy, Electron, Scanning ; Protein Precursors - biosynthesis ; Swiss 3T3 cells ; Tissue culture techniques</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-08, Vol.85 (15), p.5576-5580</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-575b7f44766d2ab57d4be94468850904e9187939ed17c92d5f43ae37be078c653</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/32196$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/32196$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2456572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watt, Fiona M.</creatorcontrib><creatorcontrib>Jordan, Peter W.</creatorcontrib><creatorcontrib>O'Neill, Charles H.</creatorcontrib><title>Cell Shape Controls Terminal Differentiation of Human Epidermal Keratinocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cultures of human epidermal keratinocytes provide a useful experimental model with which to study the factors that regulate cell proliferation and terminal differentiation. One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprived of substratum and intercellular contact. We have now investigated whether area of substratum contact, and hence cell shape, can regulate terminal differentiation. Keratinocytes were grown on circular adhesive islands that prevented cell-cell contact. By varying island area we could vary cell shape from fully spread to almost spherical. We found that when substratum contact was restricted, DNA synthesis was inhibited and expression of involucrin, a marker of terminal differentiation, was stimulated. Inhibition of proliferation was not a sufficient stimulus for involucrin synthesis in fully spread cells. When DNA synthesis and involucrin expression were plotted against contact area, classic dose-response curves were obtained. Thus cell shape acts as a signal for the terminal differentiation of keratinocytes in culture.</description><subject>B lymphocytes</subject><subject>Cell Adhesion</subject><subject>Cell Communication</subject><subject>Cell culture techniques</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cellular differentiation</subject><subject>Cultured cells</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epidermal Cells</subject><subject>Epidermis - metabolism</subject><subject>Epidermis - ultrastructure</subject><subject>Fluorescent Antibody Technique</subject><subject>foreskin</subject><subject>Humans</subject><subject>Keratinocytes</subject><subject>Keratins - physiology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Protein Precursors - biosynthesis</subject><subject>Swiss 3T3 cells</subject><subject>Tissue culture techniques</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Etv1DAUBWALUZWhsEZCAmUFq0yv3_ayGgpFbcWCsrac5EakSuJgOxL99_VohkpsYOXF-c6VfAh5Q2FLQfPzZfZpa-SWyq2UWj0jGwqW1kpYeE42AEzXRjDxgrxM6R4ArDRwSk6ZkEpqtiG3OxzH6vtPv2C1C3OOYUzVHcZpmP1YfRr6HiPOefB5CHMV-upqnfxcXS5DV1Ah1xhLNof2IWN6RU56PyZ8fXzPyI_Pl3e7q_rm25evu4ubupUgci21bHQvhFaqY76RuhMNWiGUMRIsCLTUaMstdlS3lnWyF9wj1w2CNq2S_Ix8ONxdYvi1YspuGlJbfuJnDGty2nCmQKn_QiqZFBygwPMDbGNIKWLvljhMPj44Cm6_tNsv7YwsFbdfujTeHU-vzYTdkz9OW_L3x3xf_JP-deDjP4Hr13HM-DsX-fYg71MO8YlyRq3ij8FNmcs</recordid><startdate>19880801</startdate><enddate>19880801</enddate><creator>Watt, Fiona M.</creator><creator>Jordan, Peter W.</creator><creator>O'Neill, Charles H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19880801</creationdate><title>Cell Shape Controls Terminal Differentiation of Human Epidermal Keratinocytes</title><author>Watt, Fiona M. ; Jordan, Peter W. ; O'Neill, Charles H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-575b7f44766d2ab57d4be94468850904e9187939ed17c92d5f43ae37be078c653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>B lymphocytes</topic><topic>Cell Adhesion</topic><topic>Cell Communication</topic><topic>Cell culture techniques</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cellular differentiation</topic><topic>Cultured cells</topic><topic>DNA</topic><topic>DNA - biosynthesis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epidermal Cells</topic><topic>Epidermis - metabolism</topic><topic>Epidermis - ultrastructure</topic><topic>Fluorescent Antibody Technique</topic><topic>foreskin</topic><topic>Humans</topic><topic>Keratinocytes</topic><topic>Keratins - physiology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Protein Precursors - biosynthesis</topic><topic>Swiss 3T3 cells</topic><topic>Tissue culture techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watt, Fiona M.</creatorcontrib><creatorcontrib>Jordan, Peter W.</creatorcontrib><creatorcontrib>O'Neill, Charles H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watt, Fiona M.</au><au>Jordan, Peter W.</au><au>O'Neill, Charles H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Shape Controls Terminal Differentiation of Human Epidermal Keratinocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-08-01</date><risdate>1988</risdate><volume>85</volume><issue>15</issue><spage>5576</spage><epage>5580</epage><pages>5576-5580</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cultures of human epidermal keratinocytes provide a useful experimental model with which to study the factors that regulate cell proliferation and terminal differentiation. One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprived of substratum and intercellular contact. We have now investigated whether area of substratum contact, and hence cell shape, can regulate terminal differentiation. Keratinocytes were grown on circular adhesive islands that prevented cell-cell contact. By varying island area we could vary cell shape from fully spread to almost spherical. We found that when substratum contact was restricted, DNA synthesis was inhibited and expression of involucrin, a marker of terminal differentiation, was stimulated. Inhibition of proliferation was not a sufficient stimulus for involucrin synthesis in fully spread cells. When DNA synthesis and involucrin expression were plotted against contact area, classic dose-response curves were obtained. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-08, Vol.85 (15), p.5576-5580 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | B lymphocytes Cell Adhesion Cell Communication Cell culture techniques Cell Differentiation Cell Division Cells Cells, Cultured Cellular differentiation Cultured cells DNA DNA - biosynthesis Electrophoresis, Polyacrylamide Gel Epidermal Cells Epidermis - metabolism Epidermis - ultrastructure Fluorescent Antibody Technique foreskin Humans Keratinocytes Keratins - physiology Microscopy, Electron, Scanning Protein Precursors - biosynthesis Swiss 3T3 cells Tissue culture techniques |
| title | Cell Shape Controls Terminal Differentiation of Human Epidermal Keratinocytes |
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