Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in (51)Cr-release and CD107a assays

Freshly isolated PBMC are broadly used as effector cells in functional assays that evaluate antibody-dependent cell mediated cytotoxicity (ADCC) and NK activity; however, they introduce natural-individual donor-to-donor variability. Cryopreserved PBMC provide a more consistent source of effectors th...

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Veröffentlicht in:	Journal of immunological methods 2014-04, Vol.406, p.1
Hauptverfasser:	Mata, Mariana M, Mahmood, Fareeha, Sowell, Ryan T, Baum, Linda L
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibody-Dependent Cell Cytotoxicity - immunology B-Lymphocyte Subsets - immunology CD56 Antigen - metabolism Cell Degranulation - immunology Cell Line Chromium Radioisotopes - analysis Cryopreservation Cytotoxicity Tests, Immunologic - methods Humans Killer Cells, Natural - immunology Lipopolysaccharide Receptors - metabolism Lysosomal-Associated Membrane Protein 1 - analysis Receptors, IgG - metabolism
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