Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats
Abstract The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats we...
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| Veröffentlicht in: | Renal failure 2014-03, Vol.36 (2), p.154-165 |
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| creator | Manikandan, Ramar Beulaja, Manikandan Thiagarajan, Raman Pandi, Mohan Arulvasu, Chinnasamy Prabhu, Narayanan Marimuthu Saravanan, Rajendran Esakkirajan, Masanam Palanisamy, Subramanian Dhanasekaran, Ganeshan Nisha, Rajagopalan Girijakumari Devi, Kasinathan Latha, Malaikannan |
| description | Abstract
The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol + ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-κB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-κB, thereby protecting the cells against stress induced by glycerol. |
| doi_str_mv | 10.3109/0886022X.2013.835223 |
| format | Article |
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The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol + ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-κB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-κB, thereby protecting the cells against stress induced by glycerol.</description><identifier>ISSN: 0886-022X</identifier><identifier>EISSN: 1525-6049</identifier><identifier>DOI: 10.3109/0886022X.2013.835223</identifier><identifier>PMID: 24060056</identifier><language>eng</language><publisher>England: Informa Healthcare USA, Inc</publisher><subject>Acute Kidney Injury - chemically induced ; Acute Kidney Injury - metabolism ; Acute Kidney Injury - pathology ; Animals ; Catalase - metabolism ; Coumaric Acids - pharmacology ; Creatinine - metabolism ; Ferulic acid ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Glutathione Transferase - metabolism ; Glycerol ; Immunohistochemistry ; Kidney - metabolism ; Kidney - pathology ; Lipid Peroxidation - drug effects ; Male ; nephrotoxicity ; NF-kappa B - metabolism ; Nitric Oxide - urine ; nuclear factor-kappaB ; Oxidative Stress - drug effects ; Plants ; Rats ; Rats, Wistar ; Superoxide Dismutase - metabolism ; Uric Acid - metabolism</subject><ispartof>Renal failure, 2014-03, Vol.36 (2), p.154-165</ispartof><rights>2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-5f6c1e2e85c2b849f5b56926ab15e75a6a78f2689c22ff27ad70fdd8a6c319e33</citedby><cites>FETCH-LOGICAL-c418t-5f6c1e2e85c2b849f5b56926ab15e75a6a78f2689c22ff27ad70fdd8a6c319e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24060056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manikandan, Ramar</creatorcontrib><creatorcontrib>Beulaja, Manikandan</creatorcontrib><creatorcontrib>Thiagarajan, Raman</creatorcontrib><creatorcontrib>Pandi, Mohan</creatorcontrib><creatorcontrib>Arulvasu, Chinnasamy</creatorcontrib><creatorcontrib>Prabhu, Narayanan Marimuthu</creatorcontrib><creatorcontrib>Saravanan, Rajendran</creatorcontrib><creatorcontrib>Esakkirajan, Masanam</creatorcontrib><creatorcontrib>Palanisamy, Subramanian</creatorcontrib><creatorcontrib>Dhanasekaran, Ganeshan</creatorcontrib><creatorcontrib>Nisha, Rajagopalan Girijakumari</creatorcontrib><creatorcontrib>Devi, Kasinathan</creatorcontrib><creatorcontrib>Latha, Malaikannan</creatorcontrib><title>Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats</title><title>Renal failure</title><addtitle>Ren Fail</addtitle><description>Abstract
The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol + ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-κB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-κB, thereby protecting the cells against stress induced by glycerol.</description><subject>Acute Kidney Injury - chemically induced</subject><subject>Acute Kidney Injury - metabolism</subject><subject>Acute Kidney Injury - pathology</subject><subject>Animals</subject><subject>Catalase - metabolism</subject><subject>Coumaric Acids - pharmacology</subject><subject>Creatinine - metabolism</subject><subject>Ferulic acid</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Glutathione Transferase - metabolism</subject><subject>Glycerol</subject><subject>Immunohistochemistry</subject><subject>Kidney - metabolism</subject><subject>Kidney - pathology</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>nephrotoxicity</subject><subject>NF-kappa B - metabolism</subject><subject>Nitric Oxide - urine</subject><subject>nuclear factor-kappaB</subject><subject>Oxidative Stress - drug effects</subject><subject>Plants</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Uric Acid - metabolism</subject><issn>0886-022X</issn><issn>1525-6049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi1ERZfCGyDkF8jWdmLHuYBKBQWpEpciuEUTZ7zrxRtHtgPkNfrEJFqK1EtPc_n-f0bfEPKGs23JWXPJtFZMiB9bwXi51aUUonxGNlwKWShWNc_JZkWKlTknL1M6MMalrsULci4qphiTakPur47oXYiQ3S-kaC2aTIOlFuPknaFgXE9hB25ImUYcwFM3HKboMNEj9g4y9rSb6TAZjxCpBZNDLH7COMIH2i_gsKM7PxuMwRdu6CezBAYc9zHk8McZl-elkX53KS_x5Y70ipxZ8Alf_5sX5Nunj3fXn4vbrzdfrq9uC1NxnQtpleEoUEsjOl01VnZSNUJBxyXWEhTU2gqlGyOEtaKGvma27zUoU_IGy_KCVKdeE0NKEW07RneEOLectavi9kFxuypuT4qX2NtTbJy6xcD_0IPTBXh_AtxgQzzC7xB932aYfYg2wmBcWuufXPHuUcMewee9gYjtIUxxeUJ6-sa_EKCizg</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Manikandan, Ramar</creator><creator>Beulaja, Manikandan</creator><creator>Thiagarajan, Raman</creator><creator>Pandi, Mohan</creator><creator>Arulvasu, Chinnasamy</creator><creator>Prabhu, Narayanan Marimuthu</creator><creator>Saravanan, Rajendran</creator><creator>Esakkirajan, Masanam</creator><creator>Palanisamy, Subramanian</creator><creator>Dhanasekaran, Ganeshan</creator><creator>Nisha, Rajagopalan Girijakumari</creator><creator>Devi, Kasinathan</creator><creator>Latha, Malaikannan</creator><general>Informa Healthcare USA, Inc</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140301</creationdate><title>Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats</title><author>Manikandan, Ramar ; Beulaja, Manikandan ; Thiagarajan, Raman ; Pandi, Mohan ; Arulvasu, Chinnasamy ; Prabhu, Narayanan Marimuthu ; Saravanan, Rajendran ; Esakkirajan, Masanam ; Palanisamy, Subramanian ; Dhanasekaran, Ganeshan ; Nisha, Rajagopalan Girijakumari ; Devi, Kasinathan ; Latha, Malaikannan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-5f6c1e2e85c2b849f5b56926ab15e75a6a78f2689c22ff27ad70fdd8a6c319e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acute Kidney Injury - chemically induced</topic><topic>Acute Kidney Injury - metabolism</topic><topic>Acute Kidney Injury - pathology</topic><topic>Animals</topic><topic>Catalase - metabolism</topic><topic>Coumaric Acids - pharmacology</topic><topic>Creatinine - metabolism</topic><topic>Ferulic acid</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Glutathione Transferase - metabolism</topic><topic>Glycerol</topic><topic>Immunohistochemistry</topic><topic>Kidney - metabolism</topic><topic>Kidney - pathology</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>nephrotoxicity</topic><topic>NF-kappa B - metabolism</topic><topic>Nitric Oxide - urine</topic><topic>nuclear factor-kappaB</topic><topic>Oxidative Stress - drug effects</topic><topic>Plants</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Uric Acid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manikandan, Ramar</creatorcontrib><creatorcontrib>Beulaja, Manikandan</creatorcontrib><creatorcontrib>Thiagarajan, Raman</creatorcontrib><creatorcontrib>Pandi, Mohan</creatorcontrib><creatorcontrib>Arulvasu, Chinnasamy</creatorcontrib><creatorcontrib>Prabhu, Narayanan Marimuthu</creatorcontrib><creatorcontrib>Saravanan, Rajendran</creatorcontrib><creatorcontrib>Esakkirajan, Masanam</creatorcontrib><creatorcontrib>Palanisamy, Subramanian</creatorcontrib><creatorcontrib>Dhanasekaran, Ganeshan</creatorcontrib><creatorcontrib>Nisha, Rajagopalan Girijakumari</creatorcontrib><creatorcontrib>Devi, Kasinathan</creatorcontrib><creatorcontrib>Latha, Malaikannan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Renal failure</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manikandan, Ramar</au><au>Beulaja, Manikandan</au><au>Thiagarajan, Raman</au><au>Pandi, Mohan</au><au>Arulvasu, Chinnasamy</au><au>Prabhu, Narayanan Marimuthu</au><au>Saravanan, Rajendran</au><au>Esakkirajan, Masanam</au><au>Palanisamy, Subramanian</au><au>Dhanasekaran, Ganeshan</au><au>Nisha, Rajagopalan Girijakumari</au><au>Devi, Kasinathan</au><au>Latha, Malaikannan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats</atitle><jtitle>Renal failure</jtitle><addtitle>Ren Fail</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>36</volume><issue>2</issue><spage>154</spage><epage>165</epage><pages>154-165</pages><issn>0886-022X</issn><eissn>1525-6049</eissn><abstract>Abstract
The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol + ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-κB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-κB, thereby protecting the cells against stress induced by glycerol.</abstract><cop>England</cop><pub>Informa Healthcare USA, Inc</pub><pmid>24060056</pmid><doi>10.3109/0886022X.2013.835223</doi><tpages>12</tpages></addata></record> |
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| subjects | Acute Kidney Injury - chemically induced Acute Kidney Injury - metabolism Acute Kidney Injury - pathology Animals Catalase - metabolism Coumaric Acids - pharmacology Creatinine - metabolism Ferulic acid Glutathione - metabolism Glutathione Peroxidase - metabolism Glutathione Transferase - metabolism Glycerol Immunohistochemistry Kidney - metabolism Kidney - pathology Lipid Peroxidation - drug effects Male nephrotoxicity NF-kappa B - metabolism Nitric Oxide - urine nuclear factor-kappaB Oxidative Stress - drug effects Plants Rats Rats, Wistar Superoxide Dismutase - metabolism Uric Acid - metabolism |
| title | Ameliorative effect of ferulic acid against renal injuries mediated by nuclear factor-kappaB during glycerol-induced nephrotoxicity in Wistar rats |
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