Shoot-Tip Cryopreservation by Droplet Vitrification of Byrsonima Intermedia A. Juss.: A Woody Tropical And Medicinal Plant Species from Brazilian Cerrado
Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented with 2.22 μM 6-ben...
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| Veröffentlicht in: | Cryo-Letters 2013-07, Vol.34 (4), p.338-348 |
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| creator | Coutinho Silva, Luciano Paiva, Renato Swennen, Rony Andrè, Edwige Panis, Bart |
| description | Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented
with 2.22 μM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15
days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing
to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%). |
| format | Article |
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with 2.22 μM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15
days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing
to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).</description><identifier>ISSN: 0143-2044</identifier><identifier>PMID: 23995401</identifier><language>eng</language><publisher>England: Cryoletters</publisher><subject>Benzyl Compounds ; Cryopreservation - methods ; Cryoprotective Agents - metabolism ; Kinetin - metabolism ; Malpighiaceae - drug effects ; Malpighiaceae - growth & development ; Medicinal Plant ; Meristem - drug effects ; Meristem - growth & development ; Plant Shoots - drug effects ; Plant Shoots - growth & development ; Plants, Medicinal - drug effects ; Plants, Medicinal - growth & development ; Post-Culture Effect ; Pre-Culture Effect ; Pre-Growth Effect ; Purines ; Sorbitol ; Sorbitol - metabolism ; Sucrose - metabolism ; VITRIFICATION</subject><ispartof>Cryo-Letters, 2013-07, Vol.34 (4), p.338-348</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>289,314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23995401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coutinho Silva, Luciano</creatorcontrib><creatorcontrib>Paiva, Renato</creatorcontrib><creatorcontrib>Swennen, Rony</creatorcontrib><creatorcontrib>Andrè, Edwige</creatorcontrib><creatorcontrib>Panis, Bart</creatorcontrib><title>Shoot-Tip Cryopreservation by Droplet Vitrification of Byrsonima Intermedia A. Juss.: A Woody Tropical And Medicinal Plant Species from Brazilian Cerrado</title><title>Cryo-Letters</title><addtitle>Cryo Letters</addtitle><description>Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented
with 2.22 μM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15
days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing
to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).</description><subject>Benzyl Compounds</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - metabolism</subject><subject>Kinetin - metabolism</subject><subject>Malpighiaceae - drug effects</subject><subject>Malpighiaceae - growth & development</subject><subject>Medicinal Plant</subject><subject>Meristem - drug effects</subject><subject>Meristem - growth & development</subject><subject>Plant Shoots - drug effects</subject><subject>Plant Shoots - growth & development</subject><subject>Plants, Medicinal - drug effects</subject><subject>Plants, Medicinal - growth & development</subject><subject>Post-Culture Effect</subject><subject>Pre-Culture Effect</subject><subject>Pre-Growth Effect</subject><subject>Purines</subject><subject>Sorbitol</subject><subject>Sorbitol - metabolism</subject><subject>Sucrose - metabolism</subject><subject>VITRIFICATION</subject><issn>0143-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UctO3TAQzaKovPoLlZfdBPmVh7sLt4WCgFbitl1ajj2hRrl2ajtI4U_6t5h7b5d44Zmxz5yZOfOuOMKEs5Jizg-L4xgfMaZNXYv3xSFlQlQck6Pi3_0f71O5thNahcVPASKEJ5Wsd6hf0JfgpxES-mVTsIPVuw8_oPMlRO_sRqErlyBswFiFujN0Pcd49hl16Lf3ZkHrnJ-zRtQ5g24zSFuXox-jcgndT6AtRDQEv0HnQT3b0SqHVhCCMv60OBjUGOHD3p4UPy--rlffypvvl1er7qa0jONUgtA9cNMz04uGQ1MR0lRtpZhSdUUxFg0ITnQNnJFBG5Ntq6nWpGpoS2nPTopPO94p-L8zxCQ3NmoYc4vg5ygJp6LlTcvrDP24h859nlhOIQsQFvlfzgy42wGsewCXlHz0c8gDR2m11Flf-bqS143IJ8YdlxRTggVuJMm9SgODmsckkwry4VlGlgm7Nwi3bNuL5sISbw_jewdzqULaPrEXZSagmQ</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Coutinho Silva, Luciano</creator><creator>Paiva, Renato</creator><creator>Swennen, Rony</creator><creator>Andrè, Edwige</creator><creator>Panis, Bart</creator><general>Cryoletters</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>Shoot-Tip Cryopreservation by Droplet Vitrification of Byrsonima Intermedia A. 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Juss.: A Woody Tropical And Medicinal Plant Species from Brazilian Cerrado</atitle><jtitle>Cryo-Letters</jtitle><addtitle>Cryo Letters</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>34</volume><issue>4</issue><spage>338</spage><epage>348</epage><pages>338-348</pages><issn>0143-2044</issn><abstract>Cryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented
with 2.22 μM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15
days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing
to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).</abstract><cop>England</cop><pub>Cryoletters</pub><pmid>23995401</pmid><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Ingenta Connect; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Benzyl Compounds Cryopreservation - methods Cryoprotective Agents - metabolism Kinetin - metabolism Malpighiaceae - drug effects Malpighiaceae - growth & development Medicinal Plant Meristem - drug effects Meristem - growth & development Plant Shoots - drug effects Plant Shoots - growth & development Plants, Medicinal - drug effects Plants, Medicinal - growth & development Post-Culture Effect Pre-Culture Effect Pre-Growth Effect Purines Sorbitol Sorbitol - metabolism Sucrose - metabolism VITRIFICATION |
| title | Shoot-Tip Cryopreservation by Droplet Vitrification of Byrsonima Intermedia A. Juss.: A Woody Tropical And Medicinal Plant Species from Brazilian Cerrado |
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