A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells
This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted...
Gespeichert in:
| Veröffentlicht in: | Food chemistry 2013-11, Vol.141 (2), p.1252 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | 1252 |
| container_title | Food chemistry |
| container_volume | 141 |
| creator | Tsai, Pei-Feng Ma, Chia-Yu Wu, James Swi-Bea |
| description | This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use. |
| doi_str_mv | 10.1016/j.foodchem.2013.04.024 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_23790910</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>23790910</sourcerecordid><originalsourceid>FETCH-LOGICAL-p560-8d438b7d155dca6dd985a98ec100264d65778bfa1292ac5ff84889c703a0c8103</originalsourceid><addsrcrecordid>eNo1kMtOwzAURL0A0VL4hcpLWCRcx3k4y1IBRaoEi7KuXD8St3Ec2QlV-AC-m0jAao400khnEFoSiAmQ_OEYa-ekqJWNEyA0hjSGJL1Ac6DAIkbSfIauQzgCwNSzKzRLaFFCSWCOvle4dZ-qwVUzCtd51yvTYu2dxXYItXcTbMYumK-xGgK23Fvn1UR370qc7vGjqVTjzvhs-hpX3p2nMG1tDqZ3fsRKayV6zCtu2tDjerC8xY0aTsoagT9KWmChmibcoEvNm6Bu_3KBds9Pu_Um2r69vK5X26jLcoiYTCk7FJJkmRQ8l7JkGS-ZEmQyy1OZZ0XBDpqTpEy4yLRmKWOlKIByEIwAXaDl72w3HKyS-86byWjc__9BfwATNWVf</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tsai, Pei-Feng ; Ma, Chia-Yu ; Wu, James Swi-Bea</creator><creatorcontrib>Tsai, Pei-Feng ; Ma, Chia-Yu ; Wu, James Swi-Bea</creatorcontrib><description>This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use.</description><identifier>ISSN: 0308-8146</identifier><identifier>DOI: 10.1016/j.foodchem.2013.04.024</identifier><identifier>PMID: 23790910</identifier><language>eng</language><publisher>England</publisher><subject>Agaricales - chemistry ; Amino Acid Sequence ; Cell Proliferation - drug effects ; Fungal Proteins - chemistry ; Fungal Proteins - isolation & purification ; Fungal Proteins - pharmacology ; Glycoproteins - chemistry ; Glycoproteins - isolation & purification ; Glycoproteins - pharmacology ; Growth Inhibitors - chemistry ; Growth Inhibitors - isolation & purification ; Growth Inhibitors - pharmacology ; Humans ; Leukemia - physiopathology ; Molecular Sequence Data ; Molecular Weight ; U937 Cells</subject><ispartof>Food chemistry, 2013-11, Vol.141 (2), p.1252</ispartof><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23790910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, Pei-Feng</creatorcontrib><creatorcontrib>Ma, Chia-Yu</creatorcontrib><creatorcontrib>Wu, James Swi-Bea</creatorcontrib><title>A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use.</description><subject>Agaricales - chemistry</subject><subject>Amino Acid Sequence</subject><subject>Cell Proliferation - drug effects</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Fungal Proteins - pharmacology</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - isolation & purification</subject><subject>Glycoproteins - pharmacology</subject><subject>Growth Inhibitors - chemistry</subject><subject>Growth Inhibitors - isolation & purification</subject><subject>Growth Inhibitors - pharmacology</subject><subject>Humans</subject><subject>Leukemia - physiopathology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>U937 Cells</subject><issn>0308-8146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAURL0A0VL4hcpLWCRcx3k4y1IBRaoEi7KuXD8St3Ec2QlV-AC-m0jAao400khnEFoSiAmQ_OEYa-ekqJWNEyA0hjSGJL1Ac6DAIkbSfIauQzgCwNSzKzRLaFFCSWCOvle4dZ-qwVUzCtd51yvTYu2dxXYItXcTbMYumK-xGgK23Fvn1UR370qc7vGjqVTjzvhs-hpX3p2nMG1tDqZ3fsRKayV6zCtu2tDjerC8xY0aTsoagT9KWmChmibcoEvNm6Bu_3KBds9Pu_Um2r69vK5X26jLcoiYTCk7FJJkmRQ8l7JkGS-ZEmQyy1OZZ0XBDpqTpEy4yLRmKWOlKIByEIwAXaDl72w3HKyS-86byWjc__9BfwATNWVf</recordid><startdate>20131115</startdate><enddate>20131115</enddate><creator>Tsai, Pei-Feng</creator><creator>Ma, Chia-Yu</creator><creator>Wu, James Swi-Bea</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20131115</creationdate><title>A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells</title><author>Tsai, Pei-Feng ; Ma, Chia-Yu ; Wu, James Swi-Bea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p560-8d438b7d155dca6dd985a98ec100264d65778bfa1292ac5ff84889c703a0c8103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agaricales - chemistry</topic><topic>Amino Acid Sequence</topic><topic>Cell Proliferation - drug effects</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Fungal Proteins - pharmacology</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - isolation & purification</topic><topic>Glycoproteins - pharmacology</topic><topic>Growth Inhibitors - chemistry</topic><topic>Growth Inhibitors - isolation & purification</topic><topic>Growth Inhibitors - pharmacology</topic><topic>Humans</topic><topic>Leukemia - physiopathology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, Pei-Feng</creatorcontrib><creatorcontrib>Ma, Chia-Yu</creatorcontrib><creatorcontrib>Wu, James Swi-Bea</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, Pei-Feng</au><au>Ma, Chia-Yu</au><au>Wu, James Swi-Bea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2013-11-15</date><risdate>2013</risdate><volume>141</volume><issue>2</issue><spage>1252</spage><pages>1252-</pages><issn>0308-8146</issn><abstract>This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use.</abstract><cop>England</cop><pmid>23790910</pmid><doi>10.1016/j.foodchem.2013.04.024</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0308-8146 |
| ispartof | Food chemistry, 2013-11, Vol.141 (2), p.1252 |
| issn | 0308-8146 |
| language | eng |
| recordid | cdi_pubmed_primary_23790910 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agaricales - chemistry Amino Acid Sequence Cell Proliferation - drug effects Fungal Proteins - chemistry Fungal Proteins - isolation & purification Fungal Proteins - pharmacology Glycoproteins - chemistry Glycoproteins - isolation & purification Glycoproteins - pharmacology Growth Inhibitors - chemistry Growth Inhibitors - isolation & purification Growth Inhibitors - pharmacology Humans Leukemia - physiopathology Molecular Sequence Data Molecular Weight U937 Cells |
| title | A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukemic U937 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T01%3A07%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20glycoprotein%20from%20mushroom%20Hypsizygus%20marmoreus%20(Peck)%20Bigelow%20with%20growth%20inhibitory%20effect%20against%20human%20leukemic%20U937%20cells&rft.jtitle=Food%20chemistry&rft.au=Tsai,%20Pei-Feng&rft.date=2013-11-15&rft.volume=141&rft.issue=2&rft.spage=1252&rft.pages=1252-&rft.issn=0308-8146&rft_id=info:doi/10.1016/j.foodchem.2013.04.024&rft_dat=%3Cpubmed%3E23790910%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/23790910&rfr_iscdi=true |