Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor
The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses...
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| Veröffentlicht in: | Journal of biomedical optics 2014-01, Vol.19 (1), p.011004-011004 |
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| container_title | Journal of biomedical optics |
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| creator | Chang, Ying-Feng Chao, Cheng-Han Lin, Lih-Yuan Tsai, Cheng-Han Chou, Chien Lee, Yi-Jang |
| description | The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size. |
| doi_str_mv | 10.1117/1.JBO.19.1.011004 |
| format | Article |
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We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.19.1.011004</identifier><identifier>PMID: 23788282</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Acute Kidney Injury - urine ; Adult ; Adults ; Aged ; Aged, 80 and over ; Biosensing Techniques - methods ; Biosensors ; Case-Control Studies ; Cell Line ; Cofilin 1 - urine ; Female ; Gold - chemistry ; High-Throughput Screening Assays - methods ; Humans ; Immunoassay ; Injuries ; Kidneys ; Male ; Metal Nanoparticles - chemistry ; Middle Aged ; Nanoparticles ; Oxidative Stress ; Patients ; ROC Curve ; Spectrometry, Fluorescence - methods ; Surface Plasmon Resonance - methods ; Urine</subject><ispartof>Journal of biomedical optics, 2014-01, Vol.19 (1), p.011004-011004</ispartof><rights>2014 Society of Photo-Optical Instrumentation Engineers (SPIE)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-b0b76d9e44304a70df07342027e287eb3ed8a3447d83fc5ac9785985eb4cff243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23788282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Ying-Feng</creatorcontrib><creatorcontrib>Chao, Cheng-Han</creatorcontrib><creatorcontrib>Lin, Lih-Yuan</creatorcontrib><creatorcontrib>Tsai, Cheng-Han</creatorcontrib><creatorcontrib>Chou, Chien</creatorcontrib><creatorcontrib>Lee, Yi-Jang</creatorcontrib><title>Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.</description><subject>Acute Kidney Injury - urine</subject><subject>Adult</subject><subject>Adults</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Case-Control Studies</subject><subject>Cell Line</subject><subject>Cofilin 1 - urine</subject><subject>Female</subject><subject>Gold - chemistry</subject><subject>High-Throughput Screening Assays - methods</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Injuries</subject><subject>Kidneys</subject><subject>Male</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Middle Aged</subject><subject>Nanoparticles</subject><subject>Oxidative Stress</subject><subject>Patients</subject><subject>ROC Curve</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Urine</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkstu1DAUhiMEoqXwAGyQl2wSfMnFWZa2w0WVygI2bCzHOZ548NjBjitNH4JnxqMps0AVF298jv3_n478uyheElwRQro3pPr49qYifUUqTAjG9aPilDQtLinl5HGuMWcla1t-UjyLcYMx5m3fPi1OKOs4p5yeFj8uYYGwNU4uxjvkNUrBOEDKa2ONKwmycAsWGYekSgugb2Z0sMv9JoUdStG4NZJoMuupXKbg03qa04KsV9KaOxhRTEFLBWi2Mm69K5VPs83n2iYfICpw-XIwPoKLPjwvnmhpI7y438-KL6urzxfvy-ubdx8uzq9L1fTNUg546Nqxh7pmuJYdHjXuWE0x7YDyDgYGI5esrruRM60aqfqONz1vYKiV1rRmZ8XrA3cO_nuCuIitybNYKx34FAVpW8x6jgn9B2lD8hz79VdpRtaMsqbPUnKQquBjDKDFHMxWhp0gWOzDFUTkcAXpc3EIN3te3ePTsIXx6PiVZhZUB0GcDYiNT8HlR_wjcfWQ4SjT-V9YcWdmcR4Woyx8ulz9DhHzqDPo60MgGdRkbuHo2JP-H_4TkOnnCw</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Chang, Ying-Feng</creator><creator>Chao, Cheng-Han</creator><creator>Lin, Lih-Yuan</creator><creator>Tsai, Cheng-Han</creator><creator>Chou, Chien</creator><creator>Lee, Yi-Jang</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope><scope>7QO</scope><scope>P64</scope></search><sort><creationdate>20140101</creationdate><title>Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor</title><author>Chang, Ying-Feng ; Chao, Cheng-Han ; Lin, Lih-Yuan ; Tsai, Cheng-Han ; Chou, Chien ; Lee, Yi-Jang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-b0b76d9e44304a70df07342027e287eb3ed8a3447d83fc5ac9785985eb4cff243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acute Kidney Injury - urine</topic><topic>Adult</topic><topic>Adults</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Case-Control Studies</topic><topic>Cell Line</topic><topic>Cofilin 1 - urine</topic><topic>Female</topic><topic>Gold - chemistry</topic><topic>High-Throughput Screening Assays - methods</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Injuries</topic><topic>Kidneys</topic><topic>Male</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Middle Aged</topic><topic>Nanoparticles</topic><topic>Oxidative Stress</topic><topic>Patients</topic><topic>ROC Curve</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Ying-Feng</creatorcontrib><creatorcontrib>Chao, Cheng-Han</creatorcontrib><creatorcontrib>Lin, Lih-Yuan</creatorcontrib><creatorcontrib>Tsai, Cheng-Han</creatorcontrib><creatorcontrib>Chou, Chien</creatorcontrib><creatorcontrib>Lee, Yi-Jang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Ying-Feng</au><au>Chao, Cheng-Han</au><au>Lin, Lih-Yuan</au><au>Tsai, Cheng-Han</au><au>Chou, Chien</au><au>Lee, Yi-Jang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>19</volume><issue>1</issue><spage>011004</spage><epage>011004</epage><pages>011004-011004</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. 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| subjects | Acute Kidney Injury - urine Adult Adults Aged Aged, 80 and over Biosensing Techniques - methods Biosensors Case-Control Studies Cell Line Cofilin 1 - urine Female Gold - chemistry High-Throughput Screening Assays - methods Humans Immunoassay Injuries Kidneys Male Metal Nanoparticles - chemistry Middle Aged Nanoparticles Oxidative Stress Patients ROC Curve Spectrometry, Fluorescence - methods Surface Plasmon Resonance - methods Urine |
| title | Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor |
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