Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells
Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 ( Pou5f1 ),...
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| Veröffentlicht in: | Stem cell reviews 2013-10, Vol.9 (5), p.700-708 |
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| creator | Wang, Jianyu Gu, Qi Hao, Jie Jia, Yundan Xue, Binghua Jin, Honghong Ma, Jing Wei, Renyue Hai, Tang Kong, Qingran Bou, Gerelchimeg Xia, Ping Zhou, Qi Wang, Liu Liu, Zhonghua |
| description | Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with
Oct4
(
Pou5f1
),
Sox2
,
Klf4
and
c-Myc
(termed OSKM, 4 F). Here, we found two other factors (
Tbx3
and
Nr5α2
, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly,
Nr5α2
alone could induce piPSCs formation in the presence or absence of
c-Myc
. These results suggested that
Tbx3
and
Nr5α2
may have vital roles in
Sus scrofa
and proposed new insights into pig pluripotent stem cells. |
| doi_str_mv | 10.1007/s12015-013-9439-2 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmed_primary_23625189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>23625189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2592-560aed5d5219cc91ef3ed8666e32be961a5ddc2406dc57996854ddcd259a17cb3</originalsourceid><addsrcrecordid>eNp9kNtKAzEQhoMoVqsP4I3kBaKZZJMml1o8FIoWrdchu0nLlj2RbME-li_iM5m66qVXM8x_gPkQugB6BZROriMwCoJQ4ERnXBN2gE5ACEWkotnh906JStIInca4oZSrTMExGjEumQClT9DtMn_n2DYOPwXx-cHworI7PKu7NvS26fFLW_mIywYvynXStqHs2t4n4bX3NZ76qopn6Ghlq-jPf-YYvd3fLaePZP78MJvezEnBhGZESGq9E04w0EWhwa-4d0pK6TnLvZZghXMFy6h0hZhoLZXI0sGlsIVJkfMxgqG3CG2Mwa9MF8rahp0BavY8zMDDJB5mz8OwlLkcMt02r737S_wCSAY2GGKSmrUPZtNuQ5P--Kf1CzBzalQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells</title><source>MEDLINE</source><source>SpringerLink_现刊</source><creator>Wang, Jianyu ; Gu, Qi ; Hao, Jie ; Jia, Yundan ; Xue, Binghua ; Jin, Honghong ; Ma, Jing ; Wei, Renyue ; Hai, Tang ; Kong, Qingran ; Bou, Gerelchimeg ; Xia, Ping ; Zhou, Qi ; Wang, Liu ; Liu, Zhonghua</creator><creatorcontrib>Wang, Jianyu ; Gu, Qi ; Hao, Jie ; Jia, Yundan ; Xue, Binghua ; Jin, Honghong ; Ma, Jing ; Wei, Renyue ; Hai, Tang ; Kong, Qingran ; Bou, Gerelchimeg ; Xia, Ping ; Zhou, Qi ; Wang, Liu ; Liu, Zhonghua</creatorcontrib><description>Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with
Oct4
(
Pou5f1
),
Sox2
,
Klf4
and
c-Myc
(termed OSKM, 4 F). Here, we found two other factors (
Tbx3
and
Nr5α2
, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly,
Nr5α2
alone could induce piPSCs formation in the presence or absence of
c-Myc
. These results suggested that
Tbx3
and
Nr5α2
may have vital roles in
Sus scrofa
and proposed new insights into pig pluripotent stem cells.</description><identifier>ISSN: 1550-8943</identifier><identifier>EISSN: 1558-6804</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-013-9439-2</identifier><identifier>PMID: 23625189</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Cell Biology ; Cells, Cultured ; Embryoid Bodies - cytology ; Embryoid Bodies - metabolism ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Fluorescent Antibody Technique ; Gene Expression ; Homeodomain Proteins - genetics ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; Life Sciences ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Octamer Transcription Factor-3 - genetics ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Receptors, Cytoplasmic and Nuclear - genetics ; Regenerative Medicine/Tissue Engineering ; Reverse Transcriptase Polymerase Chain Reaction ; SOXB1 Transcription Factors - genetics ; Stem Cell Transplantation - methods ; Stem Cells ; Swine ; T-Box Domain Proteins - genetics ; Teratoma - genetics ; Teratoma - metabolism ; Teratoma - pathology ; Time Factors ; Transplantation, Heterologous</subject><ispartof>Stem cell reviews, 2013-10, Vol.9 (5), p.700-708</ispartof><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2592-560aed5d5219cc91ef3ed8666e32be961a5ddc2406dc57996854ddcd259a17cb3</citedby><cites>FETCH-LOGICAL-c2592-560aed5d5219cc91ef3ed8666e32be961a5ddc2406dc57996854ddcd259a17cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23625189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jianyu</creatorcontrib><creatorcontrib>Gu, Qi</creatorcontrib><creatorcontrib>Hao, Jie</creatorcontrib><creatorcontrib>Jia, Yundan</creatorcontrib><creatorcontrib>Xue, Binghua</creatorcontrib><creatorcontrib>Jin, Honghong</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Wei, Renyue</creatorcontrib><creatorcontrib>Hai, Tang</creatorcontrib><creatorcontrib>Kong, Qingran</creatorcontrib><creatorcontrib>Bou, Gerelchimeg</creatorcontrib><creatorcontrib>Xia, Ping</creatorcontrib><creatorcontrib>Zhou, Qi</creatorcontrib><creatorcontrib>Wang, Liu</creatorcontrib><creatorcontrib>Liu, Zhonghua</creatorcontrib><title>Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells</title><title>Stem cell reviews</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with
Oct4
(
Pou5f1
),
Sox2
,
Klf4
and
c-Myc
(termed OSKM, 4 F). Here, we found two other factors (
Tbx3
and
Nr5α2
, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly,
Nr5α2
alone could induce piPSCs formation in the presence or absence of
c-Myc
. These results suggested that
Tbx3
and
Nr5α2
may have vital roles in
Sus scrofa
and proposed new insights into pig pluripotent stem cells.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Embryoid Bodies - cytology</subject><subject>Embryoid Bodies - metabolism</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression</subject><subject>Homeodomain Proteins - genetics</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Receptors, Cytoplasmic and Nuclear - genetics</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem Cells</subject><subject>Swine</subject><subject>T-Box Domain Proteins - genetics</subject><subject>Teratoma - genetics</subject><subject>Teratoma - metabolism</subject><subject>Teratoma - pathology</subject><subject>Time Factors</subject><subject>Transplantation, Heterologous</subject><issn>1550-8943</issn><issn>1558-6804</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kNtKAzEQhoMoVqsP4I3kBaKZZJMml1o8FIoWrdchu0nLlj2RbME-li_iM5m66qVXM8x_gPkQugB6BZROriMwCoJQ4ERnXBN2gE5ACEWkotnh906JStIInca4oZSrTMExGjEumQClT9DtMn_n2DYOPwXx-cHworI7PKu7NvS26fFLW_mIywYvynXStqHs2t4n4bX3NZ76qopn6Ghlq-jPf-YYvd3fLaePZP78MJvezEnBhGZESGq9E04w0EWhwa-4d0pK6TnLvZZghXMFy6h0hZhoLZXI0sGlsIVJkfMxgqG3CG2Mwa9MF8rahp0BavY8zMDDJB5mz8OwlLkcMt02r737S_wCSAY2GGKSmrUPZtNuQ5P--Kf1CzBzalQ</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Wang, Jianyu</creator><creator>Gu, Qi</creator><creator>Hao, Jie</creator><creator>Jia, Yundan</creator><creator>Xue, Binghua</creator><creator>Jin, Honghong</creator><creator>Ma, Jing</creator><creator>Wei, Renyue</creator><creator>Hai, Tang</creator><creator>Kong, Qingran</creator><creator>Bou, Gerelchimeg</creator><creator>Xia, Ping</creator><creator>Zhou, Qi</creator><creator>Wang, Liu</creator><creator>Liu, Zhonghua</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201310</creationdate><title>Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells</title><author>Wang, Jianyu ; Gu, Qi ; Hao, Jie ; Jia, Yundan ; Xue, Binghua ; Jin, Honghong ; Ma, Jing ; Wei, Renyue ; Hai, Tang ; Kong, Qingran ; Bou, Gerelchimeg ; Xia, Ping ; Zhou, Qi ; Wang, Liu ; Liu, Zhonghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2592-560aed5d5219cc91ef3ed8666e32be961a5ddc2406dc57996854ddcd259a17cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Cell Biology</topic><topic>Cells, Cultured</topic><topic>Embryoid Bodies - cytology</topic><topic>Embryoid Bodies - metabolism</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression</topic><topic>Homeodomain Proteins - genetics</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Induced Pluripotent Stem Cells - metabolism</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Receptors, Cytoplasmic and Nuclear - genetics</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem Cells</topic><topic>Swine</topic><topic>T-Box Domain Proteins - genetics</topic><topic>Teratoma - genetics</topic><topic>Teratoma - metabolism</topic><topic>Teratoma - pathology</topic><topic>Time Factors</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jianyu</creatorcontrib><creatorcontrib>Gu, Qi</creatorcontrib><creatorcontrib>Hao, Jie</creatorcontrib><creatorcontrib>Jia, Yundan</creatorcontrib><creatorcontrib>Xue, Binghua</creatorcontrib><creatorcontrib>Jin, Honghong</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Wei, Renyue</creatorcontrib><creatorcontrib>Hai, Tang</creatorcontrib><creatorcontrib>Kong, Qingran</creatorcontrib><creatorcontrib>Bou, Gerelchimeg</creatorcontrib><creatorcontrib>Xia, Ping</creatorcontrib><creatorcontrib>Zhou, Qi</creatorcontrib><creatorcontrib>Wang, Liu</creatorcontrib><creatorcontrib>Liu, Zhonghua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Stem cell reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jianyu</au><au>Gu, Qi</au><au>Hao, Jie</au><au>Jia, Yundan</au><au>Xue, Binghua</au><au>Jin, Honghong</au><au>Ma, Jing</au><au>Wei, Renyue</au><au>Hai, Tang</au><au>Kong, Qingran</au><au>Bou, Gerelchimeg</au><au>Xia, Ping</au><au>Zhou, Qi</au><au>Wang, Liu</au><au>Liu, Zhonghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells</atitle><jtitle>Stem cell reviews</jtitle><stitle>Stem Cell Rev and Rep</stitle><addtitle>Stem Cell Rev Rep</addtitle><date>2013-10</date><risdate>2013</risdate><volume>9</volume><issue>5</issue><spage>700</spage><epage>708</epage><pages>700-708</pages><issn>1550-8943</issn><eissn>1558-6804</eissn><eissn>2629-3277</eissn><abstract>Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with
Oct4
(
Pou5f1
),
Sox2
,
Klf4
and
c-Myc
(termed OSKM, 4 F). Here, we found two other factors (
Tbx3
and
Nr5α2
, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly,
Nr5α2
alone could induce piPSCs formation in the presence or absence of
c-Myc
. These results suggested that
Tbx3
and
Nr5α2
may have vital roles in
Sus scrofa
and proposed new insights into pig pluripotent stem cells.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23625189</pmid><doi>10.1007/s12015-013-9439-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1550-8943 |
| ispartof | Stem cell reviews, 2013-10, Vol.9 (5), p.700-708 |
| issn | 1550-8943 1558-6804 2629-3277 |
| language | eng |
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| source | MEDLINE; SpringerLink_现刊 |
| subjects | Alkaline Phosphatase - metabolism Animals Biomedical and Life Sciences Biomedical Engineering and Bioengineering Cell Biology Cells, Cultured Embryoid Bodies - cytology Embryoid Bodies - metabolism Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Fluorescent Antibody Technique Gene Expression Homeodomain Proteins - genetics Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Life Sciences Mice Mice, Inbred NOD Mice, SCID Octamer Transcription Factor-3 - genetics Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Receptors, Cytoplasmic and Nuclear - genetics Regenerative Medicine/Tissue Engineering Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors - genetics Stem Cell Transplantation - methods Stem Cells Swine T-Box Domain Proteins - genetics Teratoma - genetics Teratoma - metabolism Teratoma - pathology Time Factors Transplantation, Heterologous |
| title | Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells |
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