Thermal Denaturation and Renaturation of γ-Glutamyltranspeptidase of Escherichia coli
Heat-treated γ-glutamyltranspeptidase of Escherichia coli recovered enzymatic activity after incubation at 4 °C, while heat-treated γ-glutamyltranspeptidase of Bacillus subtilis did not. Fluorescent spectra, CD spectra, and native polyacrylamide gel electrophoresis analysis suggested that the dimer...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (2), p.409-412 |
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| container_end_page | 412 |
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| container_issue | 2 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 77 |
| creator | HO, Thao Van KAMEI, Kaeko WADA, Kei FUKUYAMA, Keiichi SUZUKI, Hideyuki |
| description | Heat-treated γ-glutamyltranspeptidase of Escherichia coli recovered enzymatic activity after incubation at 4 °C, while heat-treated γ-glutamyltranspeptidase of Bacillus subtilis did not. Fluorescent spectra, CD spectra, and native polyacrylamide gel electrophoresis analysis suggested that the dimer of E. coli γ-glutamyltranspeptidase was separated into protomers by heat-treatment, but was renatured by incubation at 4 °C. |
| doi_str_mv | 10.1271/bbb.120780 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (2), p.409-412 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pubmed_primary_23391932 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism denaturation Escherichia coli - enzymology Escherichia coli - genetics gamma-Glutamyltransferase - chemistry gamma-Glutamyltransferase - genetics gamma-Glutamyltransferase - metabolism Protein Denaturation Protein Multimerization Protein Refolding Protein Subunits - chemistry Protein Subunits - genetics Protein Subunits - metabolism renaturation Species Specificity Temperature γ-glutamyltranspeptidase |
| title | Thermal Denaturation and Renaturation of γ-Glutamyltranspeptidase of Escherichia coli |
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