Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods
Thymus is an important genus of the Lamiaceae family, comprising more than 400 perennial aromatic thyme species, which are used extensively for medicinal and culinary purposes. The present study focused on the development of cryopreservation procedures for Thymus vulgaris and T. cariensis, the latte...
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| Veröffentlicht in: | Cryo-Letters 2012-09, Vol.33 (5), p.363-375 |
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| description | Thymus is an important genus of the Lamiaceae family, comprising more than 400 perennial aromatic thyme species, which are used extensively for medicinal and culinary purposes. The present study focused on the development of cryopreservation procedures for Thymus vulgaris
and T. cariensis, the latter being an endemic and endangered species of Turkey. For cryopreservation of T. vulgaris shoot tips, PVS2-based one-step freezing methods, i.e., PVS2 vitrification, encapsulation-vitrification and droplet-vitrification, were compared. Cold hardening
and sucrose preculture were also optimized before the cryopreservation trials. For T. cariensis, a droplet-vitrification method was applied to cold-hardened shoot tips, and after sucrose preculture. In all the methods tested, PVS2 was applied for up to 120 min. The best T. vulgaris
cryopreservation was achieved with a droplet-vitrification method, that involved 2-weeks cold hardening of shoot cultures, 48 h preculture of shoot tips on MS medium supplemented with 0.25 M sucrose, and a 90 min PVS2 treatment in droplets. After direct immersion in LN, thawing and plating,
80% of shoot-tips recovered. Post-thaw recovery was significantly lower when the same procedure was applied to T. cariensis shoot tips; however also here 90 min PVS2 treatment produced the highest survival (25%) and recovery (25%) levels. |
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and T. cariensis, the latter being an endemic and endangered species of Turkey. For cryopreservation of T. vulgaris shoot tips, PVS2-based one-step freezing methods, i.e., PVS2 vitrification, encapsulation-vitrification and droplet-vitrification, were compared. Cold hardening
and sucrose preculture were also optimized before the cryopreservation trials. For T. cariensis, a droplet-vitrification method was applied to cold-hardened shoot tips, and after sucrose preculture. In all the methods tested, PVS2 was applied for up to 120 min. The best T. vulgaris
cryopreservation was achieved with a droplet-vitrification method, that involved 2-weeks cold hardening of shoot cultures, 48 h preculture of shoot tips on MS medium supplemented with 0.25 M sucrose, and a 90 min PVS2 treatment in droplets. After direct immersion in LN, thawing and plating,
80% of shoot-tips recovered. Post-thaw recovery was significantly lower when the same procedure was applied to T. cariensis shoot tips; however also here 90 min PVS2 treatment produced the highest survival (25%) and recovery (25%) levels.</description><identifier>ISSN: 0143-2044</identifier><identifier>PMID: 23224369</identifier><language>eng</language><publisher>Lewes: Cryoletters</publisher><subject>Animal, plant and microbial ecology ; Applied ecology ; Biological and medical sciences ; Cold Hardening ; Conservation, protection and management of environment and wildlife ; Cryopreservation - methods ; Cryoprotective Agents - metabolism ; DROPLET-VITRIFICATION ; ENCAPSULATION-VITRIFICATION ; Fundamental and applied biological sciences. Psychology ; Long-Term Conservation ; Parks, reserves, wildlife conservation. Endangered species: population survey and restocking ; Plant Shoots - growth & development ; Plant Shoots - physiology ; PVS2 VITRIFICATION ; Sucrose - metabolism ; Sucrose Preculture ; Thyme ; Thymus Plant - growth & development ; Thymus Plant - physiology ; Vitrification</subject><ispartof>Cryo-Letters, 2012-09, Vol.33 (5), p.363-375</ispartof><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>289,314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26569204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23224369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozudogru, Elif Aylin</creatorcontrib><creatorcontrib>Kaya, Ergun</creatorcontrib><title>Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods</title><title>Cryo-Letters</title><addtitle>Cryo Letters</addtitle><description>Thymus is an important genus of the Lamiaceae family, comprising more than 400 perennial aromatic thyme species, which are used extensively for medicinal and culinary purposes. The present study focused on the development of cryopreservation procedures for Thymus vulgaris
and T. cariensis, the latter being an endemic and endangered species of Turkey. For cryopreservation of T. vulgaris shoot tips, PVS2-based one-step freezing methods, i.e., PVS2 vitrification, encapsulation-vitrification and droplet-vitrification, were compared. Cold hardening
and sucrose preculture were also optimized before the cryopreservation trials. For T. cariensis, a droplet-vitrification method was applied to cold-hardened shoot tips, and after sucrose preculture. In all the methods tested, PVS2 was applied for up to 120 min. The best T. vulgaris
cryopreservation was achieved with a droplet-vitrification method, that involved 2-weeks cold hardening of shoot cultures, 48 h preculture of shoot tips on MS medium supplemented with 0.25 M sucrose, and a 90 min PVS2 treatment in droplets. After direct immersion in LN, thawing and plating,
80% of shoot-tips recovered. Post-thaw recovery was significantly lower when the same procedure was applied to T. cariensis shoot tips; however also here 90 min PVS2 treatment produced the highest survival (25%) and recovery (25%) levels.</description><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Cold Hardening</subject><subject>Conservation, protection and management of environment and wildlife</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - metabolism</subject><subject>DROPLET-VITRIFICATION</subject><subject>ENCAPSULATION-VITRIFICATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Long-Term Conservation</subject><subject>Parks, reserves, wildlife conservation. Endangered species: population survey and restocking</subject><subject>Plant Shoots - growth & development</subject><subject>Plant Shoots - physiology</subject><subject>PVS2 VITRIFICATION</subject><subject>Sucrose - metabolism</subject><subject>Sucrose Preculture</subject><subject>Thyme</subject><subject>Thymus Plant - growth & development</subject><subject>Thymus Plant - physiology</subject><subject>Vitrification</subject><issn>0143-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1P3DAQhnMoKpT2L1S-IPUS5I84WfcGq0IrQXvowtUa7MmuUWKntrPS9teT7C7ihA_2aPT4keadD8UZZZUoOa2q0-JTSs-U8qau1cfilAvOK1Grs2JYxl0YIiaMW8gueBJastrs-jERA9GhTy4R8JasLsl27NZTL5G_mxAyWbkhfSfL0A9z8_VrRCSPLkfXOrM3lteQ0JJ7zJtg0-fipIUu4Zfje1483PxYLX-Wd39ufy2v7konGppLw5k1dSVRtEoxS2uEBoxYWCHRSFwsjKLMMCvAPhkmK6kYkw0suJK1xUaJ8-LbwTvE8G_ElHXvksGuA49hTJpx0UgqqWgm9OsRHZ96tHqIroe4068pTcDFEYBkoGsjeOPSG1fLWk0xT9zVgXN-jT6Dfg5j9NOU2kwpHy5OGdd0f4Q4FlRqiHkuZsfvdxzOHAzzVuel6q0QXk5CzqiijWaSKW2xhbHLOkPU6_86VeIF7sGfMg</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Ozudogru, Elif Aylin</creator><creator>Kaya, Ergun</creator><general>Cryoletters</general><general>CryoLetters LLP</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20120901</creationdate><title>Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods</title><author>Ozudogru, Elif Aylin ; Kaya, Ergun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i370t-c21dc645e3f991d06ea7ac38d35ec5e88c901c1d3adbc154591157a82956de793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Cold Hardening</topic><topic>Conservation, protection and management of environment and wildlife</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - metabolism</topic><topic>DROPLET-VITRIFICATION</topic><topic>ENCAPSULATION-VITRIFICATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Long-Term Conservation</topic><topic>Parks, reserves, wildlife conservation. Endangered species: population survey and restocking</topic><topic>Plant Shoots - growth & development</topic><topic>Plant Shoots - physiology</topic><topic>PVS2 VITRIFICATION</topic><topic>Sucrose - metabolism</topic><topic>Sucrose Preculture</topic><topic>Thyme</topic><topic>Thymus Plant - growth & development</topic><topic>Thymus Plant - physiology</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozudogru, Elif Aylin</creatorcontrib><creatorcontrib>Kaya, Ergun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cryo-Letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozudogru, Elif Aylin</au><au>Kaya, Ergun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods</atitle><jtitle>Cryo-Letters</jtitle><addtitle>Cryo Letters</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>33</volume><issue>5</issue><spage>363</spage><epage>375</epage><pages>363-375</pages><issn>0143-2044</issn><abstract>Thymus is an important genus of the Lamiaceae family, comprising more than 400 perennial aromatic thyme species, which are used extensively for medicinal and culinary purposes. The present study focused on the development of cryopreservation procedures for Thymus vulgaris
and T. cariensis, the latter being an endemic and endangered species of Turkey. For cryopreservation of T. vulgaris shoot tips, PVS2-based one-step freezing methods, i.e., PVS2 vitrification, encapsulation-vitrification and droplet-vitrification, were compared. Cold hardening
and sucrose preculture were also optimized before the cryopreservation trials. For T. cariensis, a droplet-vitrification method was applied to cold-hardened shoot tips, and after sucrose preculture. In all the methods tested, PVS2 was applied for up to 120 min. The best T. vulgaris
cryopreservation was achieved with a droplet-vitrification method, that involved 2-weeks cold hardening of shoot cultures, 48 h preculture of shoot tips on MS medium supplemented with 0.25 M sucrose, and a 90 min PVS2 treatment in droplets. After direct immersion in LN, thawing and plating,
80% of shoot-tips recovered. Post-thaw recovery was significantly lower when the same procedure was applied to T. cariensis shoot tips; however also here 90 min PVS2 treatment produced the highest survival (25%) and recovery (25%) levels.</abstract><cop>Lewes</cop><pub>Cryoletters</pub><pmid>23224369</pmid><tpages>13</tpages></addata></record> |
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| ispartof | Cryo-Letters, 2012-09, Vol.33 (5), p.363-375 |
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| source | MEDLINE; Ingenta Connect; EZB-FREE-00999 freely available EZB journals |
| subjects | Animal, plant and microbial ecology Applied ecology Biological and medical sciences Cold Hardening Conservation, protection and management of environment and wildlife Cryopreservation - methods Cryoprotective Agents - metabolism DROPLET-VITRIFICATION ENCAPSULATION-VITRIFICATION Fundamental and applied biological sciences. Psychology Long-Term Conservation Parks, reserves, wildlife conservation. Endangered species: population survey and restocking Plant Shoots - growth & development Plant Shoots - physiology PVS2 VITRIFICATION Sucrose - metabolism Sucrose Preculture Thyme Thymus Plant - growth & development Thymus Plant - physiology Vitrification |
| title | Cryopreservation of Thymus cariensis and T. vulgaris Shoot Tips: Comparison of Three Vitrification-Based Methods |
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