Isolation and Characterization of a Novel Non-Selective β-Toxin from the Venom of the Scorpion Isometrus maculatus
Scorpion venom is composed of a number of peptides, many of which show neurotoxicity. The Isometrus maculatus scorpion, belonging to the Buthidae family, is found in many tropical regions, including the southern islands of Japan, but there have been no reports on the isolation of toxins from its ven...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2012, Vol.76 (11), p.2089-2092 |
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| container_issue | 11 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | ICHIKI, Yayoi KAWACHI, Tomoyuki MIYASHITA, Masahiro NAKAGAWA, Yoshiaki MIYAGAWA, Hisashi |
| description | Scorpion venom is composed of a number of peptides, many of which show neurotoxicity. The Isometrus maculatus scorpion, belonging to the Buthidae family, is found in many tropical regions, including the southern islands of Japan, but there have been no reports on the isolation of toxins from its venom affecting sodium channels. We isolated in this study a novel toxin, Im-2, from the I. maculatus venom. Im-2 induced paralysis in crickets after injecting 20 µg/g of body weight. Im-2 also induced lethality in mice after an intracerebroventricular injection, indicating that Im-2 had non-selective toxicity between insects and mammals. Im-2 consists of 68 amino acids cross-linked by 4 disulfide bonds, and has sequence similarity to scorpion β-toxins that have been reported to affect the sodium channels of both insects and mammals. The toxic symptoms caused by Im-2 suggest that it acted on the nervous system and shared the action mechanism(s) with sequence-homologous β-toxins. |
| doi_str_mv | 10.1271/bbb.120438 |
| format | Article |
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The Isometrus maculatus scorpion, belonging to the Buthidae family, is found in many tropical regions, including the southern islands of Japan, but there have been no reports on the isolation of toxins from its venom affecting sodium channels. We isolated in this study a novel toxin, Im-2, from the I. maculatus venom. Im-2 induced paralysis in crickets after injecting 20 µg/g of body weight. Im-2 also induced lethality in mice after an intracerebroventricular injection, indicating that Im-2 had non-selective toxicity between insects and mammals. Im-2 consists of 68 amino acids cross-linked by 4 disulfide bonds, and has sequence similarity to scorpion β-toxins that have been reported to affect the sodium channels of both insects and mammals. The toxic symptoms caused by Im-2 suggest that it acted on the nervous system and shared the action mechanism(s) with sequence-homologous β-toxins.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.120438</identifier><identifier>PMID: 23132570</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Gryllidae - drug effects ; insect toxicity ; Male ; mammalian toxicity ; Mice ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; scorpion toxin ; Scorpion Venoms - chemistry ; Scorpions ; sodium channel ; Toxins, Biological - chemistry ; Toxins, Biological - isolation & purification ; Toxins, Biological - toxicity ; β-toxin</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2012, Vol.76 (11), p.2089-2092</ispartof><rights>2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2012</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-c5aa58ebdc6078e4770c53e6c798eeabd7b691fbdfe2bb03ef652d870245adfb3</citedby><cites>FETCH-LOGICAL-c453t-c5aa58ebdc6078e4770c53e6c798eeabd7b691fbdfe2bb03ef652d870245adfb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27771337$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23132570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ICHIKI, Yayoi</creatorcontrib><creatorcontrib>KAWACHI, Tomoyuki</creatorcontrib><creatorcontrib>MIYASHITA, Masahiro</creatorcontrib><creatorcontrib>NAKAGAWA, Yoshiaki</creatorcontrib><creatorcontrib>MIYAGAWA, Hisashi</creatorcontrib><title>Isolation and Characterization of a Novel Non-Selective β-Toxin from the Venom of the Scorpion Isometrus maculatus</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Scorpion venom is composed of a number of peptides, many of which show neurotoxicity. The Isometrus maculatus scorpion, belonging to the Buthidae family, is found in many tropical regions, including the southern islands of Japan, but there have been no reports on the isolation of toxins from its venom affecting sodium channels. We isolated in this study a novel toxin, Im-2, from the I. maculatus venom. Im-2 induced paralysis in crickets after injecting 20 µg/g of body weight. Im-2 also induced lethality in mice after an intracerebroventricular injection, indicating that Im-2 had non-selective toxicity between insects and mammals. Im-2 consists of 68 amino acids cross-linked by 4 disulfide bonds, and has sequence similarity to scorpion β-toxins that have been reported to affect the sodium channels of both insects and mammals. The toxic symptoms caused by Im-2 suggest that it acted on the nervous system and shared the action mechanism(s) with sequence-homologous β-toxins.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gryllidae - drug effects</subject><subject>insect toxicity</subject><subject>Male</subject><subject>mammalian toxicity</subject><subject>Mice</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Protein Conformation</subject><subject>scorpion toxin</subject><subject>Scorpion Venoms - chemistry</subject><subject>Scorpions</subject><subject>sodium channel</subject><subject>Toxins, Biological - chemistry</subject><subject>Toxins, Biological - isolation & purification</subject><subject>Toxins, Biological - toxicity</subject><subject>β-toxin</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtOwzAQhi0EglLYcADkDRukgB3HcbpEFS8JwYLHNho7YzUoiSs7BcqxOAhnwlVa2LCZl775Z_QTcsTZGU8VP9dax4JlotgiIy4yleSTTG2TEZvwPCkyyffIfgivjMWB5LtkLxVcpFKxEQm3wTXQ166j0FV0OgMPpkdffw5DZynQe_eGTYxd8ogNmr5-Q_r9lTy5j7qj1ruW9jOkL9jFKi6smkfj_HwlEPVb7P0i0BbMIp5ahAOyY6EJeLjOY_J8dfk0vUnuHq5vpxd3icmk6BMjAWSBujI5UwVmSjEjBeZGTQpE0JXS-YRbXVlMtWYCbS7TqlAszSRUVosxOR10jXcheLTl3Nct-GXJWblyrozOlYNzET4e4PlCt1j9ohurInCyBiAYaKyHztThj1NKcSFU5OTA1Z11voV355uq7GHZOL9ZEv888AP4fYuH</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>ICHIKI, Yayoi</creator><creator>KAWACHI, Tomoyuki</creator><creator>MIYASHITA, Masahiro</creator><creator>NAKAGAWA, Yoshiaki</creator><creator>MIYAGAWA, Hisashi</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2012</creationdate><title>Isolation and Characterization of a Novel Non-Selective β-Toxin from the Venom of the Scorpion Isometrus maculatus</title><author>ICHIKI, Yayoi ; KAWACHI, Tomoyuki ; MIYASHITA, Masahiro ; NAKAGAWA, Yoshiaki ; MIYAGAWA, Hisashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-c5aa58ebdc6078e4770c53e6c798eeabd7b691fbdfe2bb03ef652d870245adfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gryllidae - drug effects</topic><topic>insect toxicity</topic><topic>Male</topic><topic>mammalian toxicity</topic><topic>Mice</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Protein Conformation</topic><topic>scorpion toxin</topic><topic>Scorpion Venoms - chemistry</topic><topic>Scorpions</topic><topic>sodium channel</topic><topic>Toxins, Biological - chemistry</topic><topic>Toxins, Biological - isolation & purification</topic><topic>Toxins, Biological - toxicity</topic><topic>β-toxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ICHIKI, Yayoi</creatorcontrib><creatorcontrib>KAWACHI, Tomoyuki</creatorcontrib><creatorcontrib>MIYASHITA, Masahiro</creatorcontrib><creatorcontrib>NAKAGAWA, Yoshiaki</creatorcontrib><creatorcontrib>MIYAGAWA, Hisashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ICHIKI, Yayoi</au><au>KAWACHI, Tomoyuki</au><au>MIYASHITA, Masahiro</au><au>NAKAGAWA, Yoshiaki</au><au>MIYAGAWA, Hisashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of a Novel Non-Selective β-Toxin from the Venom of the Scorpion Isometrus maculatus</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2012</date><risdate>2012</risdate><volume>76</volume><issue>11</issue><spage>2089</spage><epage>2092</epage><pages>2089-2092</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Scorpion venom is composed of a number of peptides, many of which show neurotoxicity. The Isometrus maculatus scorpion, belonging to the Buthidae family, is found in many tropical regions, including the southern islands of Japan, but there have been no reports on the isolation of toxins from its venom affecting sodium channels. We isolated in this study a novel toxin, Im-2, from the I. maculatus venom. Im-2 induced paralysis in crickets after injecting 20 µg/g of body weight. Im-2 also induced lethality in mice after an intracerebroventricular injection, indicating that Im-2 had non-selective toxicity between insects and mammals. Im-2 consists of 68 amino acids cross-linked by 4 disulfide bonds, and has sequence similarity to scorpion β-toxins that have been reported to affect the sodium channels of both insects and mammals. The toxic symptoms caused by Im-2 suggest that it acted on the nervous system and shared the action mechanism(s) with sequence-homologous β-toxins.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>23132570</pmid><doi>10.1271/bbb.120438</doi><tpages>4</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Gryllidae - drug effects insect toxicity Male mammalian toxicity Mice Models, Molecular Molecular Sequence Data Protein Conformation scorpion toxin Scorpion Venoms - chemistry Scorpions sodium channel Toxins, Biological - chemistry Toxins, Biological - isolation & purification Toxins, Biological - toxicity β-toxin |
| title | Isolation and Characterization of a Novel Non-Selective β-Toxin from the Venom of the Scorpion Isometrus maculatus |
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