An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol
Objective An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue. Methods Female Wistar rats were divided into six groups, each containing eight rats. The...
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| Veröffentlicht in: | Redox report : communications in free radical research 2012-09, Vol.17 (5), p.206-213 |
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| creator | Jaques, Jeandre Augusto dos Santos Rezer, João Felipe Peres Ruchel, Jader Betsch Souza, Viviane do Carmo Gonçalves Pinheiro, Kelly de Vargas Schlemmer, Karine Bizzi Schlemmer, Josiane Bizzi Bertoldo, Tatiana Montagner Dalcin Martins, Nara Maria Beck Bertoncheli, Cláudia de Mello Fontana, Márcia Camponogara Beck, Ruy Carlos Ruver Leal, Daniela Bitencourt Rosa |
| description | Objective
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.
Methods
Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO
4
. Groups 3 and 4 were sensitized with 5% NiSO
4
and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.
Results
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.
Discussion
Our results suggest that both NiSO
4
sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage. |
| doi_str_mv | 10.1179/1351000212Y.0000000024 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmed_primary_23068967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>23068967</sourcerecordid><originalsourceid>FETCH-LOGICAL-c472t-197aeadfbe598a6db2e53790b7181b252f9593dbe8689b05dea520e22d5467003</originalsourceid><addsrcrecordid>eNqFUctuFDEQtBCIhMAvRP6BCX7sjMcckFZReEiRuIAQJ6tn3GaNvPbI9m6Sz-CP8bIhhBO-uLurqlvdRcg5ZxecK_2ay54zxgQX3y7Y_ROrJ-SUq5XshBbj0xY3UncATsiLUn60SA56fE5OhGTDqAd1Sn6uI8XbBbPfYqwQ6DZZDDQ5OqeWz5VazFuovvryhl7tIexakuKBUTdI0623rbBHuuTkfMAD8NWXCplmqIXWjFDR0htfN9RlRArR0ggxYZxhKbvwG55DmrBCSeEleeYgFHx1_5-RL--uPl9-6K4_vf94ub7u5pUSteNaAYJ1E_Z6hMFOAnupNJsUH_kkeuF0r6WdcGybTqy3CL1gKITtV4Nqlzgjb499l920RTu39TMEs7RLQL4zCbz5F4l-Y76nvRlGqSTXrcFwbDDnVEpG96DlzBxMMo9MMn9NasLzx5MfZH9caYT1keCjS-36NykHayrchZRdhjj7YuR_hvwCSRml7Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Jaques, Jeandre Augusto dos Santos ; Rezer, João Felipe Peres ; Ruchel, Jader Betsch ; Souza, Viviane do Carmo Gonçalves ; Pinheiro, Kelly de Vargas ; Schlemmer, Karine Bizzi ; Schlemmer, Josiane Bizzi ; Bertoldo, Tatiana Montagner Dalcin ; Martins, Nara Maria Beck ; Bertoncheli, Cláudia de Mello ; Fontana, Márcia Camponogara ; Beck, Ruy Carlos Ruver ; Leal, Daniela Bitencourt Rosa</creator><creatorcontrib>Jaques, Jeandre Augusto dos Santos ; Rezer, João Felipe Peres ; Ruchel, Jader Betsch ; Souza, Viviane do Carmo Gonçalves ; Pinheiro, Kelly de Vargas ; Schlemmer, Karine Bizzi ; Schlemmer, Josiane Bizzi ; Bertoldo, Tatiana Montagner Dalcin ; Martins, Nara Maria Beck ; Bertoncheli, Cláudia de Mello ; Fontana, Márcia Camponogara ; Beck, Ruy Carlos Ruver ; Leal, Daniela Bitencourt Rosa</creatorcontrib><description>Objective
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.
Methods
Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO
4
. Groups 3 and 4 were sensitized with 5% NiSO
4
and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.
Results
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.
Discussion
Our results suggest that both NiSO
4
sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.</description><identifier>ISSN: 1351-0002</identifier><identifier>EISSN: 1743-2928</identifier><identifier>DOI: 10.1179/1351000212Y.0000000024</identifier><identifier>PMID: 23068967</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Animals ; Catalase - metabolism ; Clobetasol ; Clobetasol - administration & dosage ; Clobetasol - therapeutic use ; Contact dermatitis ; Dermatitis, Contact - drug therapy ; Dermatitis, Contact - metabolism ; Drug Carriers - administration & dosage ; Drug Carriers - chemistry ; Female ; Lipid Peroxidation - drug effects ; Nanostructured ; Nanostructures - administration & dosage ; Nanostructures - chemistry ; Oxidative stress ; Oxidative Stress - drug effects ; Rats ; Rats, Wistar ; Superoxide Dismutase - metabolism</subject><ispartof>Redox report : communications in free radical research, 2012-09, Vol.17 (5), p.206-213</ispartof><rights>W.S. Maney & Son Ltd 2012 2012</rights><rights>W.S. Maney & Son Ltd 2012 2012 W.S. Maney & Son Ltd 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-197aeadfbe598a6db2e53790b7181b252f9593dbe8689b05dea520e22d5467003</citedby><cites>FETCH-LOGICAL-c472t-197aeadfbe598a6db2e53790b7181b252f9593dbe8689b05dea520e22d5467003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837319/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837319/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23068967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jaques, Jeandre Augusto dos Santos</creatorcontrib><creatorcontrib>Rezer, João Felipe Peres</creatorcontrib><creatorcontrib>Ruchel, Jader Betsch</creatorcontrib><creatorcontrib>Souza, Viviane do Carmo Gonçalves</creatorcontrib><creatorcontrib>Pinheiro, Kelly de Vargas</creatorcontrib><creatorcontrib>Schlemmer, Karine Bizzi</creatorcontrib><creatorcontrib>Schlemmer, Josiane Bizzi</creatorcontrib><creatorcontrib>Bertoldo, Tatiana Montagner Dalcin</creatorcontrib><creatorcontrib>Martins, Nara Maria Beck</creatorcontrib><creatorcontrib>Bertoncheli, Cláudia de Mello</creatorcontrib><creatorcontrib>Fontana, Márcia Camponogara</creatorcontrib><creatorcontrib>Beck, Ruy Carlos Ruver</creatorcontrib><creatorcontrib>Leal, Daniela Bitencourt Rosa</creatorcontrib><title>An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol</title><title>Redox report : communications in free radical research</title><addtitle>Redox Rep</addtitle><description>Objective
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.
Methods
Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO
4
. Groups 3 and 4 were sensitized with 5% NiSO
4
and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.
Results
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.
Discussion
Our results suggest that both NiSO
4
sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.</description><subject>Animals</subject><subject>Catalase - metabolism</subject><subject>Clobetasol</subject><subject>Clobetasol - administration & dosage</subject><subject>Clobetasol - therapeutic use</subject><subject>Contact dermatitis</subject><subject>Dermatitis, Contact - drug therapy</subject><subject>Dermatitis, Contact - metabolism</subject><subject>Drug Carriers - administration & dosage</subject><subject>Drug Carriers - chemistry</subject><subject>Female</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Nanostructured</subject><subject>Nanostructures - administration & dosage</subject><subject>Nanostructures - chemistry</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Superoxide Dismutase - metabolism</subject><issn>1351-0002</issn><issn>1743-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctuFDEQtBCIhMAvRP6BCX7sjMcckFZReEiRuIAQJ6tn3GaNvPbI9m6Sz-CP8bIhhBO-uLurqlvdRcg5ZxecK_2ay54zxgQX3y7Y_ROrJ-SUq5XshBbj0xY3UncATsiLUn60SA56fE5OhGTDqAd1Sn6uI8XbBbPfYqwQ6DZZDDQ5OqeWz5VazFuovvryhl7tIexakuKBUTdI0623rbBHuuTkfMAD8NWXCplmqIXWjFDR0htfN9RlRArR0ggxYZxhKbvwG55DmrBCSeEleeYgFHx1_5-RL--uPl9-6K4_vf94ub7u5pUSteNaAYJ1E_Z6hMFOAnupNJsUH_kkeuF0r6WdcGybTqy3CL1gKITtV4Nqlzgjb499l920RTu39TMEs7RLQL4zCbz5F4l-Y76nvRlGqSTXrcFwbDDnVEpG96DlzBxMMo9MMn9NasLzx5MfZH9caYT1keCjS-36NykHayrchZRdhjj7YuR_hvwCSRml7Q</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Jaques, Jeandre Augusto dos Santos</creator><creator>Rezer, João Felipe Peres</creator><creator>Ruchel, Jader Betsch</creator><creator>Souza, Viviane do Carmo Gonçalves</creator><creator>Pinheiro, Kelly de Vargas</creator><creator>Schlemmer, Karine Bizzi</creator><creator>Schlemmer, Josiane Bizzi</creator><creator>Bertoldo, Tatiana Montagner Dalcin</creator><creator>Martins, Nara Maria Beck</creator><creator>Bertoncheli, Cláudia de Mello</creator><creator>Fontana, Márcia Camponogara</creator><creator>Beck, Ruy Carlos Ruver</creator><creator>Leal, Daniela Bitencourt Rosa</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20120901</creationdate><title>An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol</title><author>Jaques, Jeandre Augusto dos Santos ; Rezer, João Felipe Peres ; Ruchel, Jader Betsch ; Souza, Viviane do Carmo Gonçalves ; Pinheiro, Kelly de Vargas ; Schlemmer, Karine Bizzi ; Schlemmer, Josiane Bizzi ; Bertoldo, Tatiana Montagner Dalcin ; Martins, Nara Maria Beck ; Bertoncheli, Cláudia de Mello ; Fontana, Márcia Camponogara ; Beck, Ruy Carlos Ruver ; Leal, Daniela Bitencourt Rosa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-197aeadfbe598a6db2e53790b7181b252f9593dbe8689b05dea520e22d5467003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Catalase - metabolism</topic><topic>Clobetasol</topic><topic>Clobetasol - administration & dosage</topic><topic>Clobetasol - therapeutic use</topic><topic>Contact dermatitis</topic><topic>Dermatitis, Contact - drug therapy</topic><topic>Dermatitis, Contact - metabolism</topic><topic>Drug Carriers - administration & dosage</topic><topic>Drug Carriers - chemistry</topic><topic>Female</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Nanostructured</topic><topic>Nanostructures - administration & dosage</topic><topic>Nanostructures - chemistry</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jaques, Jeandre Augusto dos Santos</creatorcontrib><creatorcontrib>Rezer, João Felipe Peres</creatorcontrib><creatorcontrib>Ruchel, Jader Betsch</creatorcontrib><creatorcontrib>Souza, Viviane do Carmo Gonçalves</creatorcontrib><creatorcontrib>Pinheiro, Kelly de Vargas</creatorcontrib><creatorcontrib>Schlemmer, Karine Bizzi</creatorcontrib><creatorcontrib>Schlemmer, Josiane Bizzi</creatorcontrib><creatorcontrib>Bertoldo, Tatiana Montagner Dalcin</creatorcontrib><creatorcontrib>Martins, Nara Maria Beck</creatorcontrib><creatorcontrib>Bertoncheli, Cláudia de Mello</creatorcontrib><creatorcontrib>Fontana, Márcia Camponogara</creatorcontrib><creatorcontrib>Beck, Ruy Carlos Ruver</creatorcontrib><creatorcontrib>Leal, Daniela Bitencourt Rosa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Redox report : communications in free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jaques, Jeandre Augusto dos Santos</au><au>Rezer, João Felipe Peres</au><au>Ruchel, Jader Betsch</au><au>Souza, Viviane do Carmo Gonçalves</au><au>Pinheiro, Kelly de Vargas</au><au>Schlemmer, Karine Bizzi</au><au>Schlemmer, Josiane Bizzi</au><au>Bertoldo, Tatiana Montagner Dalcin</au><au>Martins, Nara Maria Beck</au><au>Bertoncheli, Cláudia de Mello</au><au>Fontana, Márcia Camponogara</au><au>Beck, Ruy Carlos Ruver</au><au>Leal, Daniela Bitencourt Rosa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol</atitle><jtitle>Redox report : communications in free radical research</jtitle><addtitle>Redox Rep</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>17</volume><issue>5</issue><spage>206</spage><epage>213</epage><pages>206-213</pages><issn>1351-0002</issn><eissn>1743-2928</eissn><abstract>Objective
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.
Methods
Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO
4
. Groups 3 and 4 were sensitized with 5% NiSO
4
and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.
Results
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.
Discussion
Our results suggest that both NiSO
4
sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>23068967</pmid><doi>10.1179/1351000212Y.0000000024</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Redox report : communications in free radical research, 2012-09, Vol.17 (5), p.206-213 |
| issn | 1351-0002 1743-2928 |
| language | eng |
| recordid | cdi_pubmed_primary_23068967 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Catalase - metabolism Clobetasol Clobetasol - administration & dosage Clobetasol - therapeutic use Contact dermatitis Dermatitis, Contact - drug therapy Dermatitis, Contact - metabolism Drug Carriers - administration & dosage Drug Carriers - chemistry Female Lipid Peroxidation - drug effects Nanostructured Nanostructures - administration & dosage Nanostructures - chemistry Oxidative stress Oxidative Stress - drug effects Rats Rats, Wistar Superoxide Dismutase - metabolism |
| title | An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol |
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