Using a single hydrophobic-interaction chromatography to purify pharmaceutical-grade supercoiled plasmid DNA from other isoforms

Context: The recent developments in non-viral gene therapy and DNA vaccine have fostered the development of efficient plasmid DNA (pDNA) purification processes. Objectives: This work aimed to establish a cost-effective purification process for the large-scale production of plasmid DNA for gene thera...

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Veröffentlicht in:	Pharmaceutical biology 2013-01, Vol.51 (1), p.42-48
Hauptverfasser:	Bo, Huaben, Wang, Jinquan, Chen, Qizhu, Shen, Han, Wu, Fenglin, Shao, Hongwei, Huang, Shulin
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography - economics Chromatography - methods Chromatography, High Pressure Liquid - methods Cost-Benefit Analysis DNA vaccine DNA, Superhelical - isolation & purification Escherichia coli - genetics gene therapy Genetic Therapy - methods Hydrophobic and Hydrophilic Interactions non-viral vectors Plasmids - isolation & purification Vaccines, DNA - administration & dosage
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