A novel dual-fluorescence strategy for functionally validating microRNA targets in 3' untranslated regions: regulation of the inward rectifier potassium channel K(ir)2.1 by miR-212

Gene targeting by microRNAs is important in health and disease. We developed a functional assay for identifying microRNA targets and applied it to the K(+) channel K(ir)2.1 [KCNJ2 (potassium inwardly-rectifying channel, subfamily J, member 2)] which is dysregulated in cardiac and vascular disorders....

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical journal 2012-11, Vol.448 (1), p.103
Hauptverfasser:	Goldoni, Dana, Yarham, Janet M, McGahon, Mary K, O'Connor, Anna, Guduric-Fuchs, Jasenka, Edgar, Kevin, McDonald, Denise M, Simpson, David A, Collins, Anthony
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated Regions - genetics Base Pairing Binding Sites Down-Regulation Fluorometry - methods Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics HEK293 Cells HeLa Cells Humans Luciferases - analysis Luciferases - genetics Luminescent Proteins - analysis Luminescent Proteins - genetics MicroRNAs - metabolism Mutagenesis, Site-Directed Patch-Clamp Techniques Potassium Channels, Inwardly Rectifying - metabolism Potassium Channels, Inwardly Rectifying - physiology Protein Binding Red Fluorescent Protein Reverse Transcriptase Polymerase Chain Reaction Transfection
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!