SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of th...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2012-08, Vol.109 (34), p.13853-13858 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 13858 |
|---|---|
| container_issue | 34 |
| container_start_page | 13853 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 109 |
| creator | Eloy, Nubia B Gonzalez, Nathalie Van Leene, Jelle Maleux, Katrien Vanhaeren, Hannes De Milde, Liesbeth Dhondt, Stijn Vercruysse, Leen Witters, Erwin Mercier, Raphaël Cromer, Laurence Beemster, Gerrit T. S Remaut, Han Van Montagu, Marc C. E De Jaeger, Geert Ferreira, Paulo C. G Inzé, Dirk |
| description | The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana , SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis. |
| doi_str_mv | 10.1073/pnas.1211418109 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmed_primary_22869741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>41701020</jstor_id><sourcerecordid>41701020</sourcerecordid><originalsourceid>FETCH-LOGICAL-c625t-d67c7e96760e3c49ecaeec52e0d4240a237160e76105df6a447329778cb87bcf3</originalsourceid><addsrcrecordid>eNqFkk-P0zAQxSMEYsvCmRNgiQtIZDv-0zi-IJUVsEhFHJY9W64zaV0lcbDTivIF-No4tHRhL1ySyPOb58mbl2VPKVxQkHzadyZeUEapoCUFdS-bpCfNC6HgfjYBYDIvBRNn2aMYNwCgZiU8zM4YKwslBZ1kP6_nn9_N3xBD-sZ0Qx57tK52lpjO9GsTMe-Db_3guhWxvu0b_D61e9v46FskAVfbxgw-EBeJ63a-2WGVPgia0OxJhTtsfN9iNyS9iszzYd8jGfsTEwezdI37YQbnu8fZg9o0EZ8c3-fZzYf3Xy-v8sWXj58u54vcFmw25FUhrURVyAKQW6HQGkQ7YwhV-k0wjEuaSrKgMKvqwgghOVNSlnZZyqWt-Xn29qDbb5ctVjaNFkyj--BaE_baG6f_rXRurVd-p7lgMtmcBF4fBNZ32q7mCz2eAaUqrabY0cS-Ol4W_LctxkG3LlpsktPot1HTEjgVBefs_yhwkWg1Gyd4eQfd-G3okmu_KUlFyWSipgfKBh9jwPo0LAU9ZkeP2dG32Ukdz_-25sT_CUsCyBEYO2_lVDJHU17OeEKeHZBNTKk4MYJKoMAg1V8c6rXx2qyCi_rmmkHaGVCmRKH4L2S53fk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1034714827</pqid></control><display><type>article</type><title>SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Eloy, Nubia B ; Gonzalez, Nathalie ; Van Leene, Jelle ; Maleux, Katrien ; Vanhaeren, Hannes ; De Milde, Liesbeth ; Dhondt, Stijn ; Vercruysse, Leen ; Witters, Erwin ; Mercier, Raphaël ; Cromer, Laurence ; Beemster, Gerrit T. S ; Remaut, Han ; Van Montagu, Marc C. E ; De Jaeger, Geert ; Ferreira, Paulo C. G ; Inzé, Dirk</creator><creatorcontrib>Eloy, Nubia B ; Gonzalez, Nathalie ; Van Leene, Jelle ; Maleux, Katrien ; Vanhaeren, Hannes ; De Milde, Liesbeth ; Dhondt, Stijn ; Vercruysse, Leen ; Witters, Erwin ; Mercier, Raphaël ; Cromer, Laurence ; Beemster, Gerrit T. S ; Remaut, Han ; Van Montagu, Marc C. E ; De Jaeger, Geert ; Ferreira, Paulo C. G ; Inzé, Dirk</creatorcontrib><description>The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana , SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1211418109</identifier><identifier>PMID: 22869741</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Amino Acid Sequence ; Anaphase-Promoting Complex-Cyclosome ; apical meristems ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Biological Sciences ; Cell Cycle ; Cell division ; Cell growth ; cell proliferation ; cells ; Cyclin A - chemistry ; Cyclins ; early development ; embryogenesis ; Flowers & plants ; gametogenesis ; Gene expression ; Gene Expression Regulation, Plant ; Leaves ; Life Sciences ; males ; Models, Biological ; Models, Genetic ; Molecular Sequence Data ; mutants ; Mutation ; Phenotype ; Phenotypes ; Plant cells ; plant development ; Plant Leaves - metabolism ; Plant Proteins - metabolism ; Plant reproduction ; Plants ; Pollen ; Pollen - metabolism ; Proteins ; proteolysis ; roots ; Samba ; seeds ; Sequence Homology, Amino Acid ; shoots ; ubiquitin-protein ligase ; Ubiquitin-Protein Ligase Complexes - genetics ; Ubiquitin-Protein Ligase Complexes - physiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (34), p.13853-13858</ispartof><rights>copyright © 1993-2008 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Aug 21, 2012</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c625t-d67c7e96760e3c49ecaeec52e0d4240a237160e76105df6a447329778cb87bcf3</citedby><cites>FETCH-LOGICAL-c625t-d67c7e96760e3c49ecaeec52e0d4240a237160e76105df6a447329778cb87bcf3</cites><orcidid>0000-0002-9775-4102 ; 0000-0001-6558-5669 ; 0000-0002-3946-1758 ; 0000-0001-6508-6608 ; 0000-0003-0323-2037</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/109/34.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41701020$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41701020$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22869741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01190736$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Eloy, Nubia B</creatorcontrib><creatorcontrib>Gonzalez, Nathalie</creatorcontrib><creatorcontrib>Van Leene, Jelle</creatorcontrib><creatorcontrib>Maleux, Katrien</creatorcontrib><creatorcontrib>Vanhaeren, Hannes</creatorcontrib><creatorcontrib>De Milde, Liesbeth</creatorcontrib><creatorcontrib>Dhondt, Stijn</creatorcontrib><creatorcontrib>Vercruysse, Leen</creatorcontrib><creatorcontrib>Witters, Erwin</creatorcontrib><creatorcontrib>Mercier, Raphaël</creatorcontrib><creatorcontrib>Cromer, Laurence</creatorcontrib><creatorcontrib>Beemster, Gerrit T. S</creatorcontrib><creatorcontrib>Remaut, Han</creatorcontrib><creatorcontrib>Van Montagu, Marc C. E</creatorcontrib><creatorcontrib>De Jaeger, Geert</creatorcontrib><creatorcontrib>Ferreira, Paulo C. G</creatorcontrib><creatorcontrib>Inzé, Dirk</creatorcontrib><title>SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana , SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.</description><subject>Amino Acid Sequence</subject><subject>Anaphase-Promoting Complex-Cyclosome</subject><subject>apical meristems</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological Sciences</subject><subject>Cell Cycle</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>cell proliferation</subject><subject>cells</subject><subject>Cyclin A - chemistry</subject><subject>Cyclins</subject><subject>early development</subject><subject>embryogenesis</subject><subject>Flowers & plants</subject><subject>gametogenesis</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>males</subject><subject>Models, Biological</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Plant cells</subject><subject>plant development</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - metabolism</subject><subject>Plant reproduction</subject><subject>Plants</subject><subject>Pollen</subject><subject>Pollen - metabolism</subject><subject>Proteins</subject><subject>proteolysis</subject><subject>roots</subject><subject>Samba</subject><subject>seeds</subject><subject>Sequence Homology, Amino Acid</subject><subject>shoots</subject><subject>ubiquitin-protein ligase</subject><subject>Ubiquitin-Protein Ligase Complexes - genetics</subject><subject>Ubiquitin-Protein Ligase Complexes - physiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-P0zAQxSMEYsvCmRNgiQtIZDv-0zi-IJUVsEhFHJY9W64zaV0lcbDTivIF-No4tHRhL1ySyPOb58mbl2VPKVxQkHzadyZeUEapoCUFdS-bpCfNC6HgfjYBYDIvBRNn2aMYNwCgZiU8zM4YKwslBZ1kP6_nn9_N3xBD-sZ0Qx57tK52lpjO9GsTMe-Db_3guhWxvu0b_D61e9v46FskAVfbxgw-EBeJ63a-2WGVPgia0OxJhTtsfN9iNyS9iszzYd8jGfsTEwezdI37YQbnu8fZg9o0EZ8c3-fZzYf3Xy-v8sWXj58u54vcFmw25FUhrURVyAKQW6HQGkQ7YwhV-k0wjEuaSrKgMKvqwgghOVNSlnZZyqWt-Xn29qDbb5ctVjaNFkyj--BaE_baG6f_rXRurVd-p7lgMtmcBF4fBNZ32q7mCz2eAaUqrabY0cS-Ol4W_LctxkG3LlpsktPot1HTEjgVBefs_yhwkWg1Gyd4eQfd-G3okmu_KUlFyWSipgfKBh9jwPo0LAU9ZkeP2dG32Ukdz_-25sT_CUsCyBEYO2_lVDJHU17OeEKeHZBNTKk4MYJKoMAg1V8c6rXx2qyCi_rmmkHaGVCmRKH4L2S53fk</recordid><startdate>20120821</startdate><enddate>20120821</enddate><creator>Eloy, Nubia B</creator><creator>Gonzalez, Nathalie</creator><creator>Van Leene, Jelle</creator><creator>Maleux, Katrien</creator><creator>Vanhaeren, Hannes</creator><creator>De Milde, Liesbeth</creator><creator>Dhondt, Stijn</creator><creator>Vercruysse, Leen</creator><creator>Witters, Erwin</creator><creator>Mercier, Raphaël</creator><creator>Cromer, Laurence</creator><creator>Beemster, Gerrit T. S</creator><creator>Remaut, Han</creator><creator>Van Montagu, Marc C. E</creator><creator>De Jaeger, Geert</creator><creator>Ferreira, Paulo C. G</creator><creator>Inzé, Dirk</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9775-4102</orcidid><orcidid>https://orcid.org/0000-0001-6558-5669</orcidid><orcidid>https://orcid.org/0000-0002-3946-1758</orcidid><orcidid>https://orcid.org/0000-0001-6508-6608</orcidid><orcidid>https://orcid.org/0000-0003-0323-2037</orcidid></search><sort><creationdate>20120821</creationdate><title>SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization</title><author>Eloy, Nubia B ; Gonzalez, Nathalie ; Van Leene, Jelle ; Maleux, Katrien ; Vanhaeren, Hannes ; De Milde, Liesbeth ; Dhondt, Stijn ; Vercruysse, Leen ; Witters, Erwin ; Mercier, Raphaël ; Cromer, Laurence ; Beemster, Gerrit T. S ; Remaut, Han ; Van Montagu, Marc C. E ; De Jaeger, Geert ; Ferreira, Paulo C. G ; Inzé, Dirk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c625t-d67c7e96760e3c49ecaeec52e0d4240a237160e76105df6a447329778cb87bcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Anaphase-Promoting Complex-Cyclosome</topic><topic>apical meristems</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological Sciences</topic><topic>Cell Cycle</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>cell proliferation</topic><topic>cells</topic><topic>Cyclin A - chemistry</topic><topic>Cyclins</topic><topic>early development</topic><topic>embryogenesis</topic><topic>Flowers & plants</topic><topic>gametogenesis</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>males</topic><topic>Models, Biological</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Plant cells</topic><topic>plant development</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - metabolism</topic><topic>Plant reproduction</topic><topic>Plants</topic><topic>Pollen</topic><topic>Pollen - metabolism</topic><topic>Proteins</topic><topic>proteolysis</topic><topic>roots</topic><topic>Samba</topic><topic>seeds</topic><topic>Sequence Homology, Amino Acid</topic><topic>shoots</topic><topic>ubiquitin-protein ligase</topic><topic>Ubiquitin-Protein Ligase Complexes - genetics</topic><topic>Ubiquitin-Protein Ligase Complexes - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eloy, Nubia B</creatorcontrib><creatorcontrib>Gonzalez, Nathalie</creatorcontrib><creatorcontrib>Van Leene, Jelle</creatorcontrib><creatorcontrib>Maleux, Katrien</creatorcontrib><creatorcontrib>Vanhaeren, Hannes</creatorcontrib><creatorcontrib>De Milde, Liesbeth</creatorcontrib><creatorcontrib>Dhondt, Stijn</creatorcontrib><creatorcontrib>Vercruysse, Leen</creatorcontrib><creatorcontrib>Witters, Erwin</creatorcontrib><creatorcontrib>Mercier, Raphaël</creatorcontrib><creatorcontrib>Cromer, Laurence</creatorcontrib><creatorcontrib>Beemster, Gerrit T. S</creatorcontrib><creatorcontrib>Remaut, Han</creatorcontrib><creatorcontrib>Van Montagu, Marc C. E</creatorcontrib><creatorcontrib>De Jaeger, Geert</creatorcontrib><creatorcontrib>Ferreira, Paulo C. G</creatorcontrib><creatorcontrib>Inzé, Dirk</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eloy, Nubia B</au><au>Gonzalez, Nathalie</au><au>Van Leene, Jelle</au><au>Maleux, Katrien</au><au>Vanhaeren, Hannes</au><au>De Milde, Liesbeth</au><au>Dhondt, Stijn</au><au>Vercruysse, Leen</au><au>Witters, Erwin</au><au>Mercier, Raphaël</au><au>Cromer, Laurence</au><au>Beemster, Gerrit T. S</au><au>Remaut, Han</au><au>Van Montagu, Marc C. E</au><au>De Jaeger, Geert</au><au>Ferreira, Paulo C. G</au><au>Inzé, Dirk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2012-08-21</date><risdate>2012</risdate><volume>109</volume><issue>34</issue><spage>13853</spage><epage>13858</epage><pages>13853-13858</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana , SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>22869741</pmid><doi>10.1073/pnas.1211418109</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9775-4102</orcidid><orcidid>https://orcid.org/0000-0001-6558-5669</orcidid><orcidid>https://orcid.org/0000-0002-3946-1758</orcidid><orcidid>https://orcid.org/0000-0001-6508-6608</orcidid><orcidid>https://orcid.org/0000-0003-0323-2037</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (34), p.13853-13858 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_22869741 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome apical meristems Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis thaliana Biological Sciences Cell Cycle Cell division Cell growth cell proliferation cells Cyclin A - chemistry Cyclins early development embryogenesis Flowers & plants gametogenesis Gene expression Gene Expression Regulation, Plant Leaves Life Sciences males Models, Biological Models, Genetic Molecular Sequence Data mutants Mutation Phenotype Phenotypes Plant cells plant development Plant Leaves - metabolism Plant Proteins - metabolism Plant reproduction Plants Pollen Pollen - metabolism Proteins proteolysis roots Samba seeds Sequence Homology, Amino Acid shoots ubiquitin-protein ligase Ubiquitin-Protein Ligase Complexes - genetics Ubiquitin-Protein Ligase Complexes - physiology |
| title | SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T00%3A05%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=SAMBA,%20a%20plant-specific%20anaphase-promoting%20complex/cyclosome%20regulator%20is%20involved%20in%20early%20development%20and%20A-type%20cyclin%20stabilization&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Eloy,%20Nubia%20B&rft.date=2012-08-21&rft.volume=109&rft.issue=34&rft.spage=13853&rft.epage=13858&rft.pages=13853-13858&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.1211418109&rft_dat=%3Cjstor_pubme%3E41701020%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1034714827&rft_id=info:pmid/22869741&rft_jstor_id=41701020&rfr_iscdi=true |