Interrupting the Early Region of Polyoma Virus DNA Enhances Tumorigenicity
The tumorigenicity of DNA from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-08, Vol.76 (8), p.3713-3716 |
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| container_end_page | 3716 |
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| container_issue | 8 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 76 |
| creator | Israel, Mark A. Simmons, Daniel T. Hourihan, Sara L. Rowe, Wallace P. Martin, Malcolm A. |
| description | The tumorigenicity of DNA from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with DNA I of the virus. Cell lines established in vitro from tumors induced by polyoma virions, polyoma virus DNA I, or polyoma virus DNA that had been cleaved with restriction endonucleases in the early region all contain the polyoma virus middle and small T antigens but not the large T antigen. These findings indicate that the large T antigen of polyoma virus is not required for maintenance of the transformed state and probably not for initiation of tumorigenesis by viral DNA. |
| doi_str_mv | 10.1073/pnas.76.8.3713 |
| format | Article |
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Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with DNA I of the virus. Cell lines established in vitro from tumors induced by polyoma virions, polyoma virus DNA I, or polyoma virus DNA that had been cleaved with restriction endonucleases in the early region all contain the polyoma virus middle and small T antigens but not the large T antigen. These findings indicate that the large T antigen of polyoma virus is not required for maintenance of the transformed state and probably not for initiation of tumorigenesis by viral DNA.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.76.8.3713</identifier><identifier>PMID: 226975</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antigens, Neoplasm - genetics ; Base Sequence ; Cell lines ; Cell Transformation, Viral ; Cricetinae ; DNA ; DNA, Viral - genetics ; Enzymes ; Genes, Viral ; Hamsters ; Molecular Weight ; Neoplasms, Experimental - microbiology ; Polyomavirus ; Polyomavirus - genetics ; Polyomavirus - pathogenicity ; Transformed cell line ; Tumor cell line ; Tumors ; Viral DNA ; Viral genomes ; Viral Proteins - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1979-08, Vol.76 (8), p.3713-3716</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-92531d8f0491186229b0308d3925c6cb11ad042bbc9effcd6f08197b496bfd4c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/76/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/69694$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/69694$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/226975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Israel, Mark A.</creatorcontrib><creatorcontrib>Simmons, Daniel T.</creatorcontrib><creatorcontrib>Hourihan, Sara L.</creatorcontrib><creatorcontrib>Rowe, Wallace P.</creatorcontrib><creatorcontrib>Martin, Malcolm A.</creatorcontrib><title>Interrupting the Early Region of Polyoma Virus DNA Enhances Tumorigenicity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The tumorigenicity of DNA from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with DNA I of the virus. Cell lines established in vitro from tumors induced by polyoma virions, polyoma virus DNA I, or polyoma virus DNA that had been cleaved with restriction endonucleases in the early region all contain the polyoma virus middle and small T antigens but not the large T antigen. These findings indicate that the large T antigen of polyoma virus is not required for maintenance of the transformed state and probably not for initiation of tumorigenesis by viral DNA.</description><subject>Animals</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Base Sequence</subject><subject>Cell lines</subject><subject>Cell Transformation, Viral</subject><subject>Cricetinae</subject><subject>DNA</subject><subject>DNA, Viral - genetics</subject><subject>Enzymes</subject><subject>Genes, Viral</subject><subject>Hamsters</subject><subject>Molecular Weight</subject><subject>Neoplasms, Experimental - microbiology</subject><subject>Polyomavirus</subject><subject>Polyomavirus - genetics</subject><subject>Polyomavirus - pathogenicity</subject><subject>Transformed cell line</subject><subject>Tumor cell line</subject><subject>Tumors</subject><subject>Viral DNA</subject><subject>Viral genomes</subject><subject>Viral Proteins - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1v1DAARy1EgevByoBAytQtwV9x7KFDVQ5aVAFChdVyHPvOVWIftoN6_30T3fVUFiYP7z3b-gHwFsEKwYZ83HqVqoZVvCINIs_AAkGBSkYFfA4WEOKm5BTTV-A0pTsIoag5fAleYMxEUy_A12ufTYzjNju_LvLGFCsV-13x06xd8EWwxY_Q78Kgit8ujqn49O2iWPmN8tqk4nYcQnRr4512efcanFjVJ_PmcC7Br8-r28ur8ub7l-vLi5tS07rOpcA1QR23kAqEOMNYtJBA3pEJaKZbhFQHKW5bLYy1umMWciSalgrW2o5qsgTn-3u3YzuYThufo-rlNrpBxZ0Mysl_iXcbuQ5_JeFEQDL1Z4c-hj-jSVkOLmnT98qbMCbZ0KbGs7kE1V7UMaQUjT2-gaCct5fz9rJhkst5-yl4__RnR30_9oQ_HPCcPcKn-dn_uLRj32dznyfx3V68SznEo8kEE5Q8AC6EoYs</recordid><startdate>19790801</startdate><enddate>19790801</enddate><creator>Israel, Mark A.</creator><creator>Simmons, Daniel T.</creator><creator>Hourihan, Sara L.</creator><creator>Rowe, Wallace P.</creator><creator>Martin, Malcolm A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790801</creationdate><title>Interrupting the Early Region of Polyoma Virus DNA Enhances Tumorigenicity</title><author>Israel, Mark A. ; Simmons, Daniel T. ; Hourihan, Sara L. ; Rowe, Wallace P. ; Martin, Malcolm A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-92531d8f0491186229b0308d3925c6cb11ad042bbc9effcd6f08197b496bfd4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Base Sequence</topic><topic>Cell lines</topic><topic>Cell Transformation, Viral</topic><topic>Cricetinae</topic><topic>DNA</topic><topic>DNA, Viral - genetics</topic><topic>Enzymes</topic><topic>Genes, Viral</topic><topic>Hamsters</topic><topic>Molecular Weight</topic><topic>Neoplasms, Experimental - microbiology</topic><topic>Polyomavirus</topic><topic>Polyomavirus - genetics</topic><topic>Polyomavirus - pathogenicity</topic><topic>Transformed cell line</topic><topic>Tumor cell line</topic><topic>Tumors</topic><topic>Viral DNA</topic><topic>Viral genomes</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Israel, Mark A.</creatorcontrib><creatorcontrib>Simmons, Daniel T.</creatorcontrib><creatorcontrib>Hourihan, Sara L.</creatorcontrib><creatorcontrib>Rowe, Wallace P.</creatorcontrib><creatorcontrib>Martin, Malcolm A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Israel, Mark A.</au><au>Simmons, Daniel T.</au><au>Hourihan, Sara L.</au><au>Rowe, Wallace P.</au><au>Martin, Malcolm A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interrupting the Early Region of Polyoma Virus DNA Enhances Tumorigenicity</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-08-01</date><risdate>1979</risdate><volume>76</volume><issue>8</issue><spage>3713</spage><epage>3716</epage><pages>3713-3716</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The tumorigenicity of DNA from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with DNA I of the virus. Cell lines established in vitro from tumors induced by polyoma virions, polyoma virus DNA I, or polyoma virus DNA that had been cleaved with restriction endonucleases in the early region all contain the polyoma virus middle and small T antigens but not the large T antigen. These findings indicate that the large T antigen of polyoma virus is not required for maintenance of the transformed state and probably not for initiation of tumorigenesis by viral DNA.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>226975</pmid><doi>10.1073/pnas.76.8.3713</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-08, Vol.76 (8), p.3713-3716 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigens, Neoplasm - genetics Base Sequence Cell lines Cell Transformation, Viral Cricetinae DNA DNA, Viral - genetics Enzymes Genes, Viral Hamsters Molecular Weight Neoplasms, Experimental - microbiology Polyomavirus Polyomavirus - genetics Polyomavirus - pathogenicity Transformed cell line Tumor cell line Tumors Viral DNA Viral genomes Viral Proteins - genetics |
| title | Interrupting the Early Region of Polyoma Virus DNA Enhances Tumorigenicity |
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